Hubei virga-like virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJY6|A0A1L3KJY6_9VIRU Uncharacterized protein OS=Hubei virga-like virus 7 OX=1923340 PE=4 SV=1
MM1 pKa = 7.84LYY3 pKa = 10.57LIVISALFCISSTQFVASNFEE24 pKa = 4.07LSLGEE29 pKa = 4.05IAEE32 pKa = 4.3LTDD35 pKa = 3.63GNVVFQHH42 pKa = 6.2EE43 pKa = 4.21FDD45 pKa = 4.11ILDD48 pKa = 3.38KK49 pKa = 10.76HH50 pKa = 6.81LSSEE54 pKa = 4.2VQIYY58 pKa = 7.67STNFYY63 pKa = 10.66SHH65 pKa = 7.16IVGANCPVGFHH76 pKa = 6.25RR77 pKa = 11.84QDD79 pKa = 2.95INHH82 pKa = 5.73YY83 pKa = 7.76TFSDD87 pKa = 3.35IFACIYY93 pKa = 10.33HH94 pKa = 7.48GYY96 pKa = 8.05TDD98 pKa = 4.07IYY100 pKa = 10.15SLPVKK105 pKa = 10.19YY106 pKa = 9.69RR107 pKa = 11.84AYY109 pKa = 10.83SLRR112 pKa = 11.84LTSNVIDD119 pKa = 4.18RR120 pKa = 11.84YY121 pKa = 9.74STIQYY126 pKa = 10.38INLHH130 pKa = 5.82EE131 pKa = 4.3FLVLKK136 pKa = 10.7NDD138 pKa = 3.15TGYY141 pKa = 11.07YY142 pKa = 9.93LSTRR146 pKa = 11.84FCFNEE151 pKa = 4.2YY152 pKa = 8.89PWKK155 pKa = 10.55DD156 pKa = 3.16INGTIQPHH164 pKa = 6.72LYY166 pKa = 10.35FEE168 pKa = 4.24NHH170 pKa = 5.5DD171 pKa = 4.11TFVKK175 pKa = 10.08FYY177 pKa = 11.09VSNSCLLRR185 pKa = 11.84YY186 pKa = 7.65LTTDD190 pKa = 3.46AEE192 pKa = 4.32HH193 pKa = 5.6LTYY196 pKa = 10.77YY197 pKa = 9.95IFSQIPSLFDD207 pKa = 4.52LEE209 pKa = 4.96VKK211 pKa = 9.58TPNSAVDD218 pKa = 3.78FFRR221 pKa = 11.84VGFVKK226 pKa = 8.79YY227 pKa = 9.77HH228 pKa = 6.2WLHH231 pKa = 6.05HH232 pKa = 5.4TLSPGDD238 pKa = 4.48LPYY241 pKa = 9.96MDD243 pKa = 5.4YY244 pKa = 11.24NATHH248 pKa = 7.5SITKK252 pKa = 8.86HH253 pKa = 5.78CYY255 pKa = 8.09SVEE258 pKa = 4.1YY259 pKa = 10.02CVAVYY264 pKa = 10.47YY265 pKa = 9.33PVQRR269 pKa = 11.84LFTTIAIQSAHH280 pKa = 6.52ANSFVHH286 pKa = 6.31SLTHH290 pKa = 7.29AILSILKK297 pKa = 9.29PILVEE302 pKa = 3.96ILSLMSFLFGSIIDD316 pKa = 5.1LIFSSEE322 pKa = 4.26FIDD325 pKa = 4.69LYY327 pKa = 11.67KK328 pKa = 11.0LFFDD332 pKa = 4.25NFIKK336 pKa = 10.62ALHH339 pKa = 6.21QILTFIFTNFRR350 pKa = 11.84LLIYY354 pKa = 10.53YY355 pKa = 10.35LLTNHH360 pKa = 7.13IYY362 pKa = 10.78FSMGLFLFLLIYY374 pKa = 9.9IRR376 pKa = 11.84YY377 pKa = 8.85GMLVYY382 pKa = 10.77SFIFVLLLSSLVRR395 pKa = 11.84LDD397 pKa = 3.33

Molecular weight: 46.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJY6|A0A1L3KJY6_9VIRU Uncharacterized protein OS=Hubei virga-like virus 7 OX=1923340 PE=4 SV=1
MM1 pKa = 7.54PWLIIIMLFYY11 pKa = 11.23YY12 pKa = 10.93LLTLFYY18 pKa = 11.05FNGNVMDD25 pKa = 4.0VEE27 pKa = 4.44HH28 pKa = 6.61VKK30 pKa = 10.69LFKK33 pKa = 10.36FANDD37 pKa = 4.37SIHH40 pKa = 6.05KK41 pKa = 10.35QIDD44 pKa = 3.63IVKK47 pKa = 9.91HH48 pKa = 5.3VYY50 pKa = 8.66TLQTRR55 pKa = 11.84PYY57 pKa = 8.89ILWSTDD63 pKa = 3.29VQWNPKK69 pKa = 10.13KK70 pKa = 10.48FVTVDD75 pKa = 3.24KK76 pKa = 10.89YY77 pKa = 10.81RR78 pKa = 11.84VSVTDD83 pKa = 3.36AGLYY87 pKa = 10.64GILNEE92 pKa = 4.32GAQKK96 pKa = 10.82NFNSSDD102 pKa = 3.23YY103 pKa = 11.12LRR105 pKa = 11.84FVKK108 pKa = 10.34PYY110 pKa = 10.43GLKK113 pKa = 10.48LDD115 pKa = 3.79VTKK118 pKa = 11.06SRR120 pKa = 11.84ITLLSLSS127 pKa = 3.91

Molecular weight: 14.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2795

127

2271

931.7

108.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.399 ± 0.254

2.361 ± 0.654

5.76 ± 0.344

4.651 ± 0.984

5.546 ± 1.413

2.755 ± 0.321

3.435 ± 0.573

8.229 ± 0.422

6.905 ± 1.447

9.445 ± 1.668

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.382

7.478 ± 1.259

3.327 ± 0.304

2.97 ± 0.158

3.148 ± 0.136

8.193 ± 0.488

6.047 ± 0.061

6.941 ± 0.593

0.501 ± 0.448

6.583 ± 0.662
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski