Hubei sobemo-like virus 46

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEU4|A0A1L3KEU4_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 46 OX=1923234 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 4.1EE3 pKa = 4.47AYY5 pKa = 10.64RR6 pKa = 11.84EE7 pKa = 4.52VIWQLPDD14 pKa = 3.11NYY16 pKa = 10.79LSYY19 pKa = 10.96EE20 pKa = 4.0QFLVAVSRR28 pKa = 11.84VDD30 pKa = 3.51MKK32 pKa = 11.17SSPGWPYY39 pKa = 10.98VKK41 pKa = 10.37EE42 pKa = 4.22GSTNAQILQWNGFTCSEE59 pKa = 4.21YY60 pKa = 11.37ALGRR64 pKa = 11.84MWNEE68 pKa = 3.5VNHH71 pKa = 5.92YY72 pKa = 8.88LQSDD76 pKa = 3.99EE77 pKa = 5.48PIVLSCFIKK86 pKa = 10.62SEE88 pKa = 3.74PHH90 pKa = 6.68KK91 pKa = 10.29ISKK94 pKa = 8.52VQEE97 pKa = 4.11GRR99 pKa = 11.84WRR101 pKa = 11.84LIMASPLHH109 pKa = 5.88VQIVWNMFFMYY120 pKa = 10.64LNDD123 pKa = 3.89LMVKK127 pKa = 9.88KK128 pKa = 10.12AYY130 pKa = 9.94HH131 pKa = 6.37IPSQQGLVLPNGGWKK146 pKa = 10.13AYY148 pKa = 10.25LDD150 pKa = 3.4QWRR153 pKa = 11.84SKK155 pKa = 10.94GYY157 pKa = 10.5DD158 pKa = 3.03VGLDD162 pKa = 3.34KK163 pKa = 11.12QAWDD167 pKa = 3.17WTAPIWLISMCLEE180 pKa = 3.5LRR182 pKa = 11.84YY183 pKa = 9.67RR184 pKa = 11.84LCRR187 pKa = 11.84GRR189 pKa = 11.84MRR191 pKa = 11.84GQWYY195 pKa = 9.77NIARR199 pKa = 11.84KK200 pKa = 9.61LYY202 pKa = 9.73RR203 pKa = 11.84DD204 pKa = 3.61MYY206 pKa = 10.86DD207 pKa = 3.33NPTIMLSNGQVYY219 pKa = 9.04YY220 pKa = 8.39QTIPGIEE227 pKa = 4.0KK228 pKa = 9.95SGCVNTISDD237 pKa = 3.98NSFMQIGVHH246 pKa = 6.25VLVCLDD252 pKa = 4.64LGLPLYY258 pKa = 8.29PLPVAVGDD266 pKa = 4.45DD267 pKa = 3.89TLQCASQTADD277 pKa = 2.89VEE279 pKa = 4.66AYY281 pKa = 10.02AKK283 pKa = 10.48FGAIVKK289 pKa = 9.58SASAGIEE296 pKa = 4.09FVGHH300 pKa = 6.72EE301 pKa = 4.39FTTNGPVPLYY311 pKa = 10.38LGKK314 pKa = 9.94HH315 pKa = 4.84VVKK318 pKa = 10.65SMHH321 pKa = 6.12VADD324 pKa = 5.13EE325 pKa = 4.15YY326 pKa = 11.36LPEE329 pKa = 4.5YY330 pKa = 10.33LDD332 pKa = 3.42MMCRR336 pKa = 11.84MYY338 pKa = 10.66CKK340 pKa = 10.49SPIFSYY346 pKa = 9.32WACLANEE353 pKa = 4.42LNCPVFSQDD362 pKa = 4.11YY363 pKa = 7.72YY364 pKa = 11.24SRR366 pKa = 11.84WYY368 pKa = 10.22DD369 pKa = 3.39YY370 pKa = 11.13EE371 pKa = 4.42SRR373 pKa = 11.84FF374 pKa = 3.92

Molecular weight: 43.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEU4|A0A1L3KEU4_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 46 OX=1923234 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.97KK3 pKa = 10.89FDD5 pKa = 6.14FEE7 pKa = 5.24ALMNRR12 pKa = 11.84IDD14 pKa = 4.04SLEE17 pKa = 3.95KK18 pKa = 10.74SIVPQSSGGILSHH31 pKa = 6.39LKK33 pKa = 9.74SWFEE37 pKa = 3.81NTVGRR42 pKa = 11.84FSRR45 pKa = 11.84RR46 pKa = 11.84HH47 pKa = 4.47IMYY50 pKa = 10.34AAGLAYY56 pKa = 10.08ALWLNRR62 pKa = 11.84RR63 pKa = 11.84LGPKK67 pKa = 10.03LGQLLRR73 pKa = 11.84WILSKK78 pKa = 10.71AVPGFRR84 pKa = 11.84QARR87 pKa = 11.84SWITGKK93 pKa = 10.2VEE95 pKa = 4.19ARR97 pKa = 11.84FEE99 pKa = 4.07PTRR102 pKa = 11.84LDD104 pKa = 3.14GRR106 pKa = 11.84EE107 pKa = 3.73NVMEE111 pKa = 4.57SIRR114 pKa = 11.84DD115 pKa = 3.8GSLLTPMMTPKK126 pKa = 10.39CQVSVGQMRR135 pKa = 11.84DD136 pKa = 3.43GEE138 pKa = 4.28FHH140 pKa = 6.04IHH142 pKa = 5.59GCGVRR147 pKa = 11.84LEE149 pKa = 4.45TFLVLPDD156 pKa = 4.26HH157 pKa = 6.22VWSYY161 pKa = 11.84SRR163 pKa = 11.84ISSEE167 pKa = 4.4TGRR170 pKa = 11.84TWLMGAAEE178 pKa = 4.31KK179 pKa = 10.21PIEE182 pKa = 4.12VSEE185 pKa = 4.68FEE187 pKa = 5.11ANVLDD192 pKa = 3.55TDD194 pKa = 3.79MVYY197 pKa = 10.71VRR199 pKa = 11.84LPNNVWSQLGVGTSALVHH217 pKa = 5.97EE218 pKa = 5.26VPSQGVYY225 pKa = 8.93CTIVGPATLGSCGMLRR241 pKa = 11.84HH242 pKa = 6.53DD243 pKa = 4.36PYY245 pKa = 10.76VFGRR249 pKa = 11.84VVYY252 pKa = 10.53DD253 pKa = 3.84GSTTGGFSGAAYY265 pKa = 8.95MRR267 pKa = 11.84ANSIAGIHH275 pKa = 4.8QRR277 pKa = 11.84GGKK280 pKa = 6.57TCNGGFSVSYY290 pKa = 10.44LWVTLCAMEE299 pKa = 4.38KK300 pKa = 9.59MKK302 pKa = 10.98PEE304 pKa = 4.24DD305 pKa = 3.32TADD308 pKa = 3.07WLRR311 pKa = 11.84NSFKK315 pKa = 10.15GKK317 pKa = 9.9KK318 pKa = 8.67RR319 pKa = 11.84VRR321 pKa = 11.84VDD323 pKa = 3.48KK324 pKa = 10.99SWADD328 pKa = 3.28TDD330 pKa = 4.02TVRR333 pKa = 11.84VMVDD337 pKa = 2.86GAFAIVEE344 pKa = 4.1RR345 pKa = 11.84SSMKK349 pKa = 10.1QAFGSNYY356 pKa = 9.58QDD358 pKa = 3.88DD359 pKa = 5.03LEE361 pKa = 4.58DD362 pKa = 4.0FKK364 pKa = 11.74VPRR367 pKa = 11.84EE368 pKa = 4.1LGYY371 pKa = 10.95EE372 pKa = 4.02DD373 pKa = 4.67AVNEE377 pKa = 4.18SGEE380 pKa = 4.43VKK382 pKa = 10.47SLKK385 pKa = 10.25QPGALSVVQNTQEE398 pKa = 4.32EE399 pKa = 4.68EE400 pKa = 4.15TSPVLILTNGFKK412 pKa = 10.57RR413 pKa = 11.84LSTEE417 pKa = 3.67QQNMVMGEE425 pKa = 3.95LNALRR430 pKa = 11.84KK431 pKa = 7.81ITNIRR436 pKa = 11.84KK437 pKa = 8.2MALQSKK443 pKa = 8.94AASSSIVTDD452 pKa = 3.5SSSTQQAA459 pKa = 3.05

Molecular weight: 50.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

833

374

459

416.5

47.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.122 ± 0.309

2.041 ± 0.549

4.922 ± 0.096

5.642 ± 0.343

3.481 ± 0.166

7.563 ± 0.862

1.921 ± 0.133

4.442 ± 0.389

4.802 ± 0.156

8.643 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.082 ± 0.12

3.962 ± 0.193

4.202 ± 0.536

4.322 ± 0.299

5.642 ± 0.832

8.403 ± 0.722

4.322 ± 1.005

8.043 ± 0.339

2.881 ± 0.526

4.562 ± 1.62
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski