Etheostoma spectabile (orangethroat darter)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala;

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18371 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J5DIY8|A0A5J5DIY8_9PERO Uncharacterized protein OS=Etheostoma spectabile OX=54343 GN=FQN60_009397 PE=4 SV=1
MM1 pKa = 7.73DD2 pKa = 5.74HH3 pKa = 6.77SPSIITQVTRR13 pKa = 11.84DD14 pKa = 3.57EE15 pKa = 4.6EE16 pKa = 5.03DD17 pKa = 3.16NATCRR22 pKa = 11.84EE23 pKa = 4.08DD24 pKa = 3.4GANEE28 pKa = 3.7VSTSKK33 pKa = 10.59KK34 pKa = 8.14PRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GLLDD42 pKa = 3.85SLFCCLCRR50 pKa = 11.84KK51 pKa = 9.23EE52 pKa = 5.13SEE54 pKa = 4.28PPPLKK59 pKa = 10.92NNAPLLVEE67 pKa = 4.34EE68 pKa = 5.1NGTLSKK74 pKa = 10.43QCKK77 pKa = 9.54PSDD80 pKa = 3.63QQFEE84 pKa = 4.41SSDD87 pKa = 3.87FEE89 pKa = 5.82PDD91 pKa = 3.17TLAVNHH97 pKa = 7.1DD98 pKa = 3.83GFGQCEE104 pKa = 4.05MTDD107 pKa = 4.4FIPEE111 pKa = 4.35CTDD114 pKa = 3.35HH115 pKa = 8.14LDD117 pKa = 3.65FLQHH121 pKa = 6.31YY122 pKa = 7.21EE123 pKa = 4.09PCDD126 pKa = 3.53PQCEE130 pKa = 4.28YY131 pKa = 11.13FEE133 pKa = 5.97PDD135 pKa = 3.85TSTEE139 pKa = 3.88DD140 pKa = 3.35CKK142 pKa = 10.88QCEE145 pKa = 4.26RR146 pKa = 11.84TGFTPNVSDD155 pKa = 5.82SEE157 pKa = 4.57DD158 pKa = 3.65LLDD161 pKa = 5.15CGTEE165 pKa = 3.62HH166 pKa = 7.47FEE168 pKa = 5.02YY169 pKa = 10.83DD170 pKa = 3.31EE171 pKa = 4.61CTNDD175 pKa = 5.61DD176 pKa = 5.33DD177 pKa = 7.3DD178 pKa = 7.36DD179 pKa = 6.42DD180 pKa = 6.72DD181 pKa = 6.66YY182 pKa = 12.15YY183 pKa = 11.65DD184 pKa = 6.18DD185 pKa = 5.89DD186 pKa = 6.84DD187 pKa = 6.05NDD189 pKa = 3.73EE190 pKa = 4.39TCQPEE195 pKa = 4.37QEE197 pKa = 4.7DD198 pKa = 3.88EE199 pKa = 4.37HH200 pKa = 8.57NEE202 pKa = 3.83AEE204 pKa = 4.96PIDD207 pKa = 4.25EE208 pKa = 4.32EE209 pKa = 5.18SFTLSEE215 pKa = 4.73DD216 pKa = 4.06VNSSDD221 pKa = 3.37YY222 pKa = 9.29DD223 pKa = 4.07TPVHH227 pKa = 6.7VYY229 pKa = 10.1FDD231 pKa = 4.09DD232 pKa = 5.26RR233 pKa = 11.84EE234 pKa = 4.23DD235 pKa = 3.45VYY237 pKa = 10.92FASEE241 pKa = 4.11CCEE244 pKa = 3.92THH246 pKa = 6.69QLTEE250 pKa = 4.21VNSPHH255 pKa = 6.84DD256 pKa = 4.36TLNMSADD263 pKa = 3.43HH264 pKa = 7.25CEE266 pKa = 3.97MCEE269 pKa = 3.75TDD271 pKa = 3.94YY272 pKa = 11.77SEE274 pKa = 4.44TSQEE278 pKa = 3.79YY279 pKa = 10.1DD280 pKa = 3.43QQCATSKK287 pKa = 10.06QCSHH291 pKa = 6.24KK292 pKa = 9.14TSEE295 pKa = 4.26FGMEE299 pKa = 3.97EE300 pKa = 4.71DD301 pKa = 4.54GSSDD305 pKa = 3.44CSSIEE310 pKa = 3.94TKK312 pKa = 10.48SFKK315 pKa = 9.84TCPDD319 pKa = 2.84GSIPSDD325 pKa = 3.08ICSDD329 pKa = 3.83SSGEE333 pKa = 4.25SQKK336 pKa = 7.85TTQEE340 pKa = 4.13YY341 pKa = 10.78SSDD344 pKa = 3.34EE345 pKa = 4.14HH346 pKa = 5.35THH348 pKa = 5.65WEE350 pKa = 4.15SFEE353 pKa = 4.09EE354 pKa = 4.46DD355 pKa = 3.98DD356 pKa = 5.63EE357 pKa = 5.52IEE359 pKa = 3.99QSSINGSNEE368 pKa = 3.77DD369 pKa = 3.41QTITPTVNSVIEE381 pKa = 3.98DD382 pKa = 3.59HH383 pKa = 7.38FDD385 pKa = 3.31LFDD388 pKa = 4.57RR389 pKa = 11.84ADD391 pKa = 3.29YY392 pKa = 9.85WGNTFAQKK400 pKa = 10.1QRR402 pKa = 11.84YY403 pKa = 8.24ISCFDD408 pKa = 3.58GGDD411 pKa = 3.0IHH413 pKa = 7.65DD414 pKa = 4.39RR415 pKa = 11.84LHH417 pKa = 8.05LEE419 pKa = 4.28EE420 pKa = 4.54VQSEE424 pKa = 4.25AQKK427 pKa = 10.48CAKK430 pKa = 9.72NVYY433 pKa = 9.33KK434 pKa = 10.65FKK436 pKa = 10.37EE437 pKa = 3.89IKK439 pKa = 9.15KK440 pKa = 8.49TIDD443 pKa = 3.29VLEE446 pKa = 4.57TCLDD450 pKa = 3.7APEE453 pKa = 4.38EE454 pKa = 4.34ACEE457 pKa = 3.92DD458 pKa = 3.98TYY460 pKa = 11.79EE461 pKa = 4.21EE462 pKa = 4.98DD463 pKa = 3.81ASQRR467 pKa = 11.84DD468 pKa = 3.82DD469 pKa = 4.21ASTGSCEE476 pKa = 4.58SEE478 pKa = 3.97EE479 pKa = 4.72QAEE482 pKa = 4.18DD483 pKa = 3.65WIKK486 pKa = 11.04EE487 pKa = 4.3SGSSLSGDD495 pKa = 3.22EE496 pKa = 4.28VFVDD500 pKa = 4.15EE501 pKa = 5.23SEE503 pKa = 4.54VFALYY508 pKa = 10.5EE509 pKa = 4.21EE510 pKa = 4.52NCEE513 pKa = 4.0EE514 pKa = 5.41DD515 pKa = 4.07EE516 pKa = 4.19DD517 pKa = 4.45TALDD521 pKa = 3.58GHH523 pKa = 5.38VTEE526 pKa = 5.63IYY528 pKa = 10.46NEE530 pKa = 4.06EE531 pKa = 4.0EE532 pKa = 4.66AEE534 pKa = 4.25VCLPSGNKK542 pKa = 9.69EE543 pKa = 4.22SMSAPCADD551 pKa = 5.01DD552 pKa = 3.64ISVEE556 pKa = 4.14EE557 pKa = 4.34VEE559 pKa = 4.97PYY561 pKa = 9.52WLLVDD566 pKa = 4.08HH567 pKa = 7.09EE568 pKa = 4.76EE569 pKa = 4.14NEE571 pKa = 4.55EE572 pKa = 3.98IVITQLTPHH581 pKa = 6.67EE582 pKa = 4.58ARR584 pKa = 11.84TEE586 pKa = 4.0EE587 pKa = 4.19NKK589 pKa = 10.62ASEE592 pKa = 4.49STDD595 pKa = 3.14SDD597 pKa = 3.28EE598 pKa = 6.31DD599 pKa = 3.41EE600 pKa = 4.36WSDD603 pKa = 3.89EE604 pKa = 4.0ISTEE608 pKa = 3.94LCEE611 pKa = 5.05CEE613 pKa = 4.36YY614 pKa = 10.59CIPPIEE620 pKa = 4.34EE621 pKa = 4.12VPAKK625 pKa = 10.26PLLPRR630 pKa = 11.84MKK632 pKa = 9.86STDD635 pKa = 3.05AGKK638 pKa = 9.43ICVVIDD644 pKa = 3.71LDD646 pKa = 3.74EE647 pKa = 4.47TLVHH651 pKa = 6.68SSFKK655 pKa = 10.96VYY657 pKa = 9.99VLKK660 pKa = 10.64RR661 pKa = 11.84PHH663 pKa = 7.15VDD665 pKa = 2.95EE666 pKa = 4.17FLKK669 pKa = 10.99RR670 pKa = 11.84MGEE673 pKa = 3.96LFEE676 pKa = 5.41CVLFTASLSKK686 pKa = 11.06YY687 pKa = 9.64ADD689 pKa = 3.7PVSDD693 pKa = 6.39LLDD696 pKa = 2.94KK697 pKa = 10.75WGAFRR702 pKa = 11.84SRR704 pKa = 11.84LFRR707 pKa = 11.84EE708 pKa = 3.9SCVFHH713 pKa = 6.89KK714 pKa = 10.9GNYY717 pKa = 9.11VKK719 pKa = 10.65DD720 pKa = 3.95LSRR723 pKa = 11.84LGRR726 pKa = 11.84DD727 pKa = 3.29LNKK730 pKa = 10.25VIIIDD735 pKa = 3.96NSPASYY741 pKa = 10.77VFHH744 pKa = 7.92PDD746 pKa = 2.61NAVPVASWFDD756 pKa = 3.67DD757 pKa = 3.6MSDD760 pKa = 3.58TEE762 pKa = 5.18LLDD765 pKa = 4.78LIPFFEE771 pKa = 4.75RR772 pKa = 11.84LSKK775 pKa = 10.86VDD777 pKa = 5.0DD778 pKa = 4.11IYY780 pKa = 11.59EE781 pKa = 4.03VLQQQRR787 pKa = 11.84TSSS790 pKa = 3.19

Molecular weight: 89.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J5D9M3|A0A5J5D9M3_9PERO TAFH domain-containing protein OS=Etheostoma spectabile OX=54343 GN=FQN60_001790 PE=3 SV=1
MM1 pKa = 8.12RR2 pKa = 11.84ITSVLRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84AWWHH14 pKa = 4.69GRR16 pKa = 11.84ATRR19 pKa = 11.84HH20 pKa = 6.2HH21 pKa = 6.98LFQRR25 pKa = 11.84GTT27 pKa = 3.29

Molecular weight: 3.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18371

0

18371

8925540

9

17660

485.8

54.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.639 ± 0.014

2.274 ± 0.015

5.078 ± 0.014

6.768 ± 0.027

3.459 ± 0.014

6.46 ± 0.021

2.723 ± 0.011

4.18 ± 0.015

5.463 ± 0.022

9.543 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.008

3.764 ± 0.01

5.911 ± 0.021

4.804 ± 0.021

5.751 ± 0.014

8.934 ± 0.025

5.741 ± 0.017

6.335 ± 0.02

1.183 ± 0.007

2.593 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski