Hyphomicrobium sp. (strain MC1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Hyphomicrobium; unclassified Hyphomicrobium

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4889 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8JCV3|F8JCV3_HYPSM Response regulator receiver (CheY-like protein) (Modular protein) OS=Hyphomicrobium sp. (strain MC1) OX=717785 GN=HYPMC_3264 PE=4 SV=1
MM1 pKa = 7.35QIANLGSKK9 pKa = 10.1LVASNFDD16 pKa = 3.3ADD18 pKa = 4.02EE19 pKa = 4.41PLDD22 pKa = 4.69RR23 pKa = 11.84IGICGNSEE31 pKa = 3.78LYY33 pKa = 10.56YY34 pKa = 8.24WTGKK38 pKa = 10.61VGVEE42 pKa = 4.88GYY44 pKa = 11.29ADD46 pKa = 3.7DD47 pKa = 4.12FF48 pKa = 4.75

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8JDL0|F8JDL0_HYPSM Probable cytosol aminopeptidase OS=Hyphomicrobium sp. (strain MC1) OX=717785 GN=pepA PE=3 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84SRR4 pKa = 11.84SGMRR8 pKa = 11.84KK9 pKa = 9.26AGAGRR14 pKa = 11.84GRR16 pKa = 11.84RR17 pKa = 11.84STGRR21 pKa = 11.84LAGLVKK27 pKa = 10.83AGGTGQHH34 pKa = 5.9GRR36 pKa = 11.84AQLSRR41 pKa = 11.84SQRR44 pKa = 11.84RR45 pKa = 11.84GKK47 pKa = 9.75QAVVQAIPAKK57 pKa = 10.0ALKK60 pKa = 9.98RR61 pKa = 11.84PNYY64 pKa = 9.5LRR66 pKa = 11.84CKK68 pKa = 10.2

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4889

0

4889

1411884

11

2106

288.8

31.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.842 ± 0.044

0.907 ± 0.012

5.693 ± 0.028

5.529 ± 0.035

3.788 ± 0.025

8.057 ± 0.036

2.026 ± 0.017

5.584 ± 0.029

4.171 ± 0.028

9.684 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.016

2.916 ± 0.02

5.19 ± 0.028

3.137 ± 0.025

6.668 ± 0.036

6.079 ± 0.027

5.455 ± 0.022

7.246 ± 0.031

1.329 ± 0.017

2.322 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski