Shuttleworthia satelles DSM 14600

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Shuttleworthia; Shuttleworthia satelles

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2247 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4GC82|C4GC82_9FIRM Guanylate kinase-like domain-containing protein OS=Shuttleworthia satelles DSM 14600 OX=626523 GN=GCWU000342_01570 PE=4 SV=1
MM1 pKa = 7.84AEE3 pKa = 3.78NNAPVVNKK11 pKa = 9.36DD12 pKa = 3.34TMIGEE17 pKa = 4.79LLQIDD22 pKa = 4.33TEE24 pKa = 4.21LAPILMSIGMHH35 pKa = 6.49CLGCPSSQMEE45 pKa = 4.76TIAEE49 pKa = 3.94AAMVHH54 pKa = 6.87GYY56 pKa = 10.25DD57 pKa = 4.67ADD59 pKa = 4.16DD60 pKa = 5.11LVDD63 pKa = 5.13EE64 pKa = 4.76INSYY68 pKa = 11.12LSAKK72 pKa = 9.94AASQPAQAA80 pKa = 4.92

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4G9M2|C4G9M2_9FIRM Cation diffusion facilitator family transporter OS=Shuttleworthia satelles DSM 14600 OX=626523 GN=GCWU000342_00675 PE=4 SV=1
MM1 pKa = 7.67PRR3 pKa = 11.84RR4 pKa = 11.84AINQSSPLRR13 pKa = 11.84NRR15 pKa = 11.84SASKK19 pKa = 10.06IQSLSHH25 pKa = 5.52IRR27 pKa = 11.84AEE29 pKa = 4.67FSAVRR34 pKa = 11.84NFLNDD39 pKa = 2.98LCKK42 pKa = 10.56YY43 pKa = 9.33DD44 pKa = 3.42FCKK47 pKa = 10.79

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2247

0

2247

649359

37

2978

289.0

32.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.303 ± 0.067

1.305 ± 0.026

6.027 ± 0.046

7.044 ± 0.062

4.004 ± 0.035

7.457 ± 0.048

1.9 ± 0.023

6.894 ± 0.048

5.901 ± 0.054

9.36 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.051 ± 0.03

3.698 ± 0.044

3.531 ± 0.033

3.534 ± 0.03

5.966 ± 0.066

6.215 ± 0.041

4.824 ± 0.064

6.394 ± 0.043

0.884 ± 0.019

3.706 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski