Intrasporangium chromatireducens Q5-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Intrasporangium; Intrasporangium chromatireducens

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4363 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W9GP50|W9GP50_9MICO Single-stranded DNA-binding protein OS=Intrasporangium chromatireducens Q5-1 OX=584657 GN=N864_17790 PE=4 SV=1
MM1 pKa = 7.64SEE3 pKa = 4.45LSYY6 pKa = 11.14PDD8 pKa = 3.71DD9 pKa = 3.57LRR11 pKa = 11.84YY12 pKa = 8.68TSDD15 pKa = 3.67HH16 pKa = 5.49EE17 pKa = 4.42WVRR20 pKa = 11.84GDD22 pKa = 3.26GDD24 pKa = 3.96TVRR27 pKa = 11.84VGITAFAQDD36 pKa = 3.34ALGDD40 pKa = 3.82VVYY43 pKa = 11.12VSLPAVGDD51 pKa = 3.76ALAVGDD57 pKa = 3.78ACGEE61 pKa = 4.21VEE63 pKa = 4.37STKK66 pKa = 10.72SVSDD70 pKa = 3.08IFAPVAGEE78 pKa = 3.93VTAVNDD84 pKa = 4.22ALDD87 pKa = 3.97ATPEE91 pKa = 4.17LVNSDD96 pKa = 4.07PYY98 pKa = 11.04GDD100 pKa = 2.9GWMFEE105 pKa = 4.02VRR107 pKa = 11.84PDD109 pKa = 3.55DD110 pKa = 4.73AAVLDD115 pKa = 4.62DD116 pKa = 5.56LMDD119 pKa = 4.5AAAYY123 pKa = 8.13QASLSSS129 pKa = 3.44

Molecular weight: 13.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W9GQE8|W9GQE8_9MICO Signal peptidase I OS=Intrasporangium chromatireducens Q5-1 OX=584657 GN=N864_24095 PE=3 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84RR3 pKa = 11.84ATPRR7 pKa = 11.84PRR9 pKa = 11.84PVPSLRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84SSRR20 pKa = 11.84LAPEE24 pKa = 4.62GWAGRR29 pKa = 11.84KK30 pKa = 9.39GLGGPAATAVGPSSPRR46 pKa = 11.84ARR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84LVAPARR56 pKa = 11.84HH57 pKa = 5.81ARR59 pKa = 11.84SSRR62 pKa = 11.84RR63 pKa = 11.84GAGTNGARR71 pKa = 11.84PALAMTWPTKK81 pKa = 9.53RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84PMPSTT89 pKa = 3.42

Molecular weight: 9.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4363

0

4363

1380227

37

2113

316.3

33.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.135 ± 0.051

0.706 ± 0.01

6.025 ± 0.033

5.583 ± 0.03

2.755 ± 0.02

9.235 ± 0.028

2.298 ± 0.017

3.757 ± 0.027

2.008 ± 0.03

10.24 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.851 ± 0.016

1.764 ± 0.02

5.693 ± 0.025

2.952 ± 0.018

7.857 ± 0.042

5.304 ± 0.024

6.143 ± 0.031

9.241 ± 0.035

1.553 ± 0.015

1.901 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski