Cronobacter phage CR5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Derbicusvirus; unclassified Derbicusvirus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 231 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4IHZ3|R4IHZ3_9CAUD Putative DNA-directed RNA polymerase beta' subunit OS=Cronobacter phage CR5 OX=1195085 GN=CR5_024 PE=4 SV=1
MM1 pKa = 7.35SRR3 pKa = 11.84RR4 pKa = 11.84VLYY7 pKa = 9.87PVMDD11 pKa = 5.94AIHH14 pKa = 7.05AYY16 pKa = 9.93GVEE19 pKa = 4.19SFAALIRR26 pKa = 11.84EE27 pKa = 4.06NLVEE31 pKa = 4.98GLDD34 pKa = 3.75GVNIEE39 pKa = 4.71VFDD42 pKa = 4.27DD43 pKa = 3.8ANFYY47 pKa = 10.92RR48 pKa = 11.84VFAYY52 pKa = 10.14AYY54 pKa = 9.23IAGAMSGSSQVFLDD68 pKa = 3.98AFGVDD73 pKa = 3.29NLEE76 pKa = 4.59PLDD79 pKa = 3.94LHH81 pKa = 6.8PNVPGIKK88 pKa = 8.95TGQFTLDD95 pKa = 3.56GDD97 pKa = 4.01DD98 pKa = 3.67TVNFSLDD105 pKa = 3.9DD106 pKa = 4.57DD107 pKa = 4.98PNDD110 pKa = 4.7LINSEE115 pKa = 4.39VATFILPFTVDD126 pKa = 2.75SSEE129 pKa = 4.2ADD131 pKa = 3.51TVEE134 pKa = 4.03VTSRR138 pKa = 11.84YY139 pKa = 9.86VDD141 pKa = 3.43MLNKK145 pKa = 10.07EE146 pKa = 4.75LFTQTGLVSVIRR158 pKa = 11.84SMFPGQYY165 pKa = 8.96IDD167 pKa = 4.14YY168 pKa = 10.49RR169 pKa = 11.84GDD171 pKa = 3.64DD172 pKa = 4.23LLSMVVVCDD181 pKa = 3.51VV182 pKa = 3.19

Molecular weight: 20.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4IFE7|R4IFE7_9CAUD Uncharacterized protein OS=Cronobacter phage CR5 OX=1195085 GN=CR5_123 PE=4 SV=1
MM1 pKa = 7.55LKK3 pKa = 9.83YY4 pKa = 10.7LSWALVEE11 pKa = 3.97LAMFILVGLGMVLSILPALCFMIGFAGQEE40 pKa = 3.6RR41 pKa = 11.84AEE43 pKa = 4.12FEE45 pKa = 4.29RR46 pKa = 11.84VKK48 pKa = 10.79LRR50 pKa = 11.84SEE52 pKa = 3.81LGIRR56 pKa = 11.84WRR58 pKa = 11.84EE59 pKa = 3.65RR60 pKa = 11.84NRR62 pKa = 11.84AANQRR67 pKa = 11.84ACGKK71 pKa = 10.16RR72 pKa = 11.84PNN74 pKa = 3.68

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

231

0

231

70062

57

2474

303.3

34.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.06 ± 0.181

0.826 ± 0.053

6.44 ± 0.104

6.312 ± 0.178

4.121 ± 0.079

6.42 ± 0.178

1.975 ± 0.084

5.625 ± 0.094

5.464 ± 0.138

8.876 ± 0.12

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.085

5.04 ± 0.136

4.365 ± 0.092

3.551 ± 0.079

5.616 ± 0.13

5.793 ± 0.115

6.483 ± 0.152

7.021 ± 0.124

1.337 ± 0.051

3.931 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski