Escherichia phage vB_EcoM_005

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Dhakavirus; unclassified Dhakavirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0ILW7|A0A3T0ILW7_9CAUD Translation repressor protein OS=Escherichia phage vB_EcoM_005 OX=2500761 GN=vBEcoM005_176 PE=4 SV=1
MM1 pKa = 7.61SIAIYY6 pKa = 9.97IKK8 pKa = 10.57SEE10 pKa = 3.94SCDD13 pKa = 3.48SYY15 pKa = 11.4LYY17 pKa = 10.85SYY19 pKa = 9.43DD20 pKa = 3.4TGTSEE25 pKa = 4.97EE26 pKa = 4.51KK27 pKa = 10.36IKK29 pKa = 11.03EE30 pKa = 4.04DD31 pKa = 4.65LEE33 pKa = 5.54SDD35 pKa = 2.92MDD37 pKa = 4.09MFCPIGDD44 pKa = 3.88YY45 pKa = 9.86MLQVSDD51 pKa = 4.47SEE53 pKa = 4.86SPSSEE58 pKa = 3.34NRR60 pKa = 11.84LQIIMQDD67 pKa = 2.7IFKK70 pKa = 10.65QSWEE74 pKa = 4.19RR75 pKa = 11.84DD76 pKa = 3.45DD77 pKa = 3.98EE78 pKa = 4.36

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0ILG7|A0A3T0ILG7_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM_005 OX=2500761 GN=vBEcoM005_010 PE=4 SV=1
MM1 pKa = 7.53AKK3 pKa = 10.16KK4 pKa = 9.46EE5 pKa = 4.17VKK7 pKa = 10.55VVDD10 pKa = 4.16GTSKK14 pKa = 9.78RR15 pKa = 11.84AGYY18 pKa = 9.8KK19 pKa = 9.18RR20 pKa = 11.84ASNKK24 pKa = 10.11RR25 pKa = 11.84INQVVDD31 pKa = 3.24KK32 pKa = 10.27LQARR36 pKa = 11.84ARR38 pKa = 11.84AVLRR42 pKa = 11.84ADD44 pKa = 3.85AACFGKK50 pKa = 9.41PQAA53 pKa = 4.58

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

250

0

250

46537

53

888

186.1

21.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.977 ± 0.207

1.031 ± 0.071

6.335 ± 0.126

7.375 ± 0.196

4.401 ± 0.11

6.318 ± 0.224

1.747 ± 0.082

6.82 ± 0.115

7.671 ± 0.223

7.609 ± 0.136

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.748 ± 0.093

5.329 ± 0.124

3.533 ± 0.098

3.301 ± 0.1

4.401 ± 0.11

6.279 ± 0.153

5.688 ± 0.182

6.724 ± 0.138

1.386 ± 0.072

4.328 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski