Vibrio phage phiVa1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 135 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410T6Z5|A0A410T6Z5_9CAUD Homing endonuclease OS=Vibrio phage phiVa1 OX=2508852 GN=ValB1MD_319 PE=4 SV=1
MM1 pKa = 7.69ARR3 pKa = 11.84DD4 pKa = 3.88LKK6 pKa = 11.31NIFDD10 pKa = 5.01SITPEE15 pKa = 4.17NIKK18 pKa = 10.35DD19 pKa = 3.52VPLIEE24 pKa = 4.72DD25 pKa = 3.37AMSIFIEE32 pKa = 4.26SLEE35 pKa = 4.09EE36 pKa = 3.82LSKK39 pKa = 11.26EE40 pKa = 4.26SIDD43 pKa = 3.84VKK45 pKa = 10.73NVYY48 pKa = 10.35SNEE51 pKa = 4.08IIRR54 pKa = 11.84EE55 pKa = 4.03EE56 pKa = 4.35LIKK59 pKa = 10.31IYY61 pKa = 10.69LDD63 pKa = 3.2DD64 pKa = 4.78LYY66 pKa = 11.29RR67 pKa = 11.84VFKK70 pKa = 10.75NIQLNQKK77 pKa = 9.92IIDD80 pKa = 4.84RR81 pKa = 11.84IDD83 pKa = 3.5SLNGIYY89 pKa = 10.66GNNDD93 pKa = 2.58QYY95 pKa = 11.7FNKK98 pKa = 10.42DD99 pKa = 3.31AILNMASYY107 pKa = 11.29LNDD110 pKa = 3.25EE111 pKa = 4.51HH112 pKa = 8.23FMTIKK117 pKa = 10.5AFKK120 pKa = 9.46EE121 pKa = 4.2SKK123 pKa = 9.08GTEE126 pKa = 3.63KK127 pKa = 10.59AIEE130 pKa = 4.04YY131 pKa = 8.14MYY133 pKa = 11.23SLISVFVSPSDD144 pKa = 3.44NGEE147 pKa = 3.98SFQLIEE153 pKa = 4.7NDD155 pKa = 3.26VFDD158 pKa = 3.71YY159 pKa = 9.96TIRR162 pKa = 11.84GEE164 pKa = 4.18LPEE167 pKa = 4.56EE168 pKa = 4.16FYY170 pKa = 11.48NYY172 pKa = 9.3IVAPLAHH179 pKa = 7.23PLGFTYY185 pKa = 9.72TYY187 pKa = 9.73EE188 pKa = 4.13QYY190 pKa = 11.33LSLVLEE196 pKa = 4.63DD197 pKa = 5.26FFPGEE202 pKa = 4.2NISYY206 pKa = 9.87DD207 pKa = 3.35INALEE212 pKa = 4.23VRR214 pKa = 11.84CLFPDD219 pKa = 3.99GTTKK223 pKa = 9.4VTDD226 pKa = 4.04FKK228 pKa = 11.48YY229 pKa = 11.04YY230 pKa = 10.35YY231 pKa = 9.87DD232 pKa = 3.77EE233 pKa = 4.61NGNIIKK239 pKa = 10.24DD240 pKa = 3.79EE241 pKa = 4.4NGTPVQRR248 pKa = 11.84VVVDD252 pKa = 3.25VTVINDD258 pKa = 2.97VGEE261 pKa = 4.06RR262 pKa = 11.84RR263 pKa = 11.84KK264 pKa = 10.58KK265 pKa = 10.33ILFEE269 pKa = 5.28DD270 pKa = 4.01GTYY273 pKa = 11.02LLQVSLINGRR283 pKa = 11.84TNVYY287 pKa = 10.37FMDD290 pKa = 4.52EE291 pKa = 3.73NDD293 pKa = 3.26EE294 pKa = 4.51TIKK297 pKa = 11.03HH298 pKa = 6.5FDD300 pKa = 3.69QQCSIYY306 pKa = 10.5FEE308 pKa = 4.99YY309 pKa = 10.56EE310 pKa = 3.65FNYY313 pKa = 9.15DD314 pKa = 3.26TTLGEE319 pKa = 4.31EE320 pKa = 4.69FSSEE324 pKa = 4.12DD325 pKa = 3.45TKK327 pKa = 11.07STSDD331 pKa = 3.11YY332 pKa = 10.53FARR335 pKa = 11.84LNSDD339 pKa = 3.01AAAIYY344 pKa = 10.05IGLEE348 pKa = 4.14DD349 pKa = 6.12DD350 pKa = 4.13PTLDD354 pKa = 5.27DD355 pKa = 3.9IGEE358 pKa = 4.15FFIGNQLDD366 pKa = 3.8LFDD369 pKa = 5.35IGFNLIRR376 pKa = 11.84DD377 pKa = 3.59RR378 pKa = 11.84DD379 pKa = 4.08GNNLIPSGMQGEE391 pKa = 4.14EE392 pKa = 4.03HH393 pKa = 6.31GRR395 pKa = 11.84KK396 pKa = 8.0EE397 pKa = 4.6HH398 pKa = 6.14YY399 pKa = 9.88FSPSEE404 pKa = 3.99TADD407 pKa = 3.59LKK409 pKa = 11.44VLLGKK414 pKa = 9.02YY415 pKa = 9.34EE416 pKa = 4.13KK417 pKa = 10.88DD418 pKa = 2.76VDD420 pKa = 3.11IFYY423 pKa = 10.78NGEE426 pKa = 3.94YY427 pKa = 10.73LEE429 pKa = 4.46TQHH432 pKa = 7.41IFYY435 pKa = 10.2TDD437 pKa = 4.1IINPSYY443 pKa = 10.76QVVEE447 pKa = 4.26RR448 pKa = 11.84VNSITKK454 pKa = 9.84YY455 pKa = 9.48QEE457 pKa = 3.75QNIGNEE463 pKa = 4.08DD464 pKa = 3.9YY465 pKa = 10.76LAHH468 pKa = 6.12EE469 pKa = 4.48HH470 pKa = 6.14MIEE473 pKa = 4.31NIEE476 pKa = 4.37SLATYY481 pKa = 9.81NQITLEE487 pKa = 3.89PAYY490 pKa = 10.82VGTHH494 pKa = 5.49NFIDD498 pKa = 3.8ANGVNRR504 pKa = 11.84GAFPGYY510 pKa = 9.57MIIGGNYY517 pKa = 10.25DD518 pKa = 3.39FDD520 pKa = 4.81RR521 pKa = 11.84SQTEE525 pKa = 4.02VEE527 pKa = 4.16QVIGYY532 pKa = 10.07DD533 pKa = 3.3PFTNDD538 pKa = 2.42GTFIGGYY545 pKa = 9.87SDD547 pKa = 4.35DD548 pKa = 3.49QDD550 pKa = 4.09YY551 pKa = 11.95NEE553 pKa = 5.48DD554 pKa = 3.35LSQTVNPNEE563 pKa = 4.07SIIYY567 pKa = 10.51FEE569 pKa = 4.7TNVDD573 pKa = 3.41ADD575 pKa = 4.04SVNLQVEE582 pKa = 4.75TYY584 pKa = 10.89DD585 pKa = 3.85VFDD588 pKa = 4.46CGVYY592 pKa = 9.93RR593 pKa = 11.84DD594 pKa = 4.09GVLLEE599 pKa = 5.17DD600 pKa = 4.22NNISALSS607 pKa = 3.5

Molecular weight: 69.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410T6I8|A0A410T6I8_9CAUD DUF2493 domain-containing protein OS=Vibrio phage phiVa1 OX=2508852 GN=ValB1MD_021 PE=4 SV=1
MM1 pKa = 7.2ARR3 pKa = 11.84KK4 pKa = 8.62VQKK7 pKa = 9.98EE8 pKa = 4.04LTPAEE13 pKa = 4.07SVKK16 pKa = 10.29IVKK19 pKa = 10.39KK20 pKa = 10.36LVKK23 pKa = 9.99DD24 pKa = 3.49KK25 pKa = 11.34KK26 pKa = 10.5RR27 pKa = 11.84INKK30 pKa = 9.39ADD32 pKa = 3.68WVPGNIIYY40 pKa = 8.97TYY42 pKa = 11.04YY43 pKa = 10.33LAKK46 pKa = 10.9DD47 pKa = 3.26MTQVYY52 pKa = 10.61DD53 pKa = 3.63RR54 pKa = 11.84TPLVLILRR62 pKa = 11.84RR63 pKa = 11.84NKK65 pKa = 8.79THH67 pKa = 6.17TLGLNFHH74 pKa = 7.6WIPFKK79 pKa = 10.83MRR81 pKa = 11.84DD82 pKa = 3.16NLARR86 pKa = 11.84YY87 pKa = 8.66ILKK90 pKa = 10.41LNKK93 pKa = 9.8KK94 pKa = 9.83NIEE97 pKa = 3.83QGKK100 pKa = 8.26PLEE103 pKa = 3.98FSYY106 pKa = 10.95RR107 pKa = 11.84QLKK110 pKa = 9.83PILKK114 pKa = 9.1NYY116 pKa = 9.81GYY118 pKa = 10.63APCIRR123 pKa = 11.84LYY125 pKa = 10.18INHH128 pKa = 7.4RR129 pKa = 11.84FRR131 pKa = 11.84SSGTVIPPDD140 pKa = 3.21RR141 pKa = 11.84LVEE144 pKa = 4.09VARR147 pKa = 11.84LRR149 pKa = 11.84TEE151 pKa = 3.82TFTKK155 pKa = 10.46GKK157 pKa = 8.3YY158 pKa = 7.31TAEE161 pKa = 3.75QLYY164 pKa = 10.82AKK166 pKa = 9.89AVKK169 pKa = 10.18DD170 pKa = 3.44GRR172 pKa = 11.84RR173 pKa = 11.84RR174 pKa = 11.84AKK176 pKa = 9.76ARR178 pKa = 11.84KK179 pKa = 8.92RR180 pKa = 11.84KK181 pKa = 9.37KK182 pKa = 10.26

Molecular weight: 21.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

135

0

135

37587

61

896

278.4

31.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.972 ± 0.253

1.019 ± 0.063

6.837 ± 0.157

7.665 ± 0.201

4.781 ± 0.146

5.733 ± 0.17

1.916 ± 0.109

7.284 ± 0.18

8.343 ± 0.266

7.926 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.109

6.377 ± 0.166

3.11 ± 0.114

3.693 ± 0.1

3.852 ± 0.129

6.901 ± 0.209

5.47 ± 0.183

6.023 ± 0.138

1.014 ± 0.062

4.451 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski