Gordonia phage Twinkle

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6ENC7|A0A4Y6ENC7_9CAUD Major capsid protein OS=Gordonia phage Twinkle OX=2583043 GN=6 PE=4 SV=1
MM1 pKa = 6.87GTIVYY6 pKa = 10.33GGGDD10 pKa = 3.7HH11 pKa = 6.62TLSLPDD17 pKa = 3.51SAVARR22 pKa = 11.84IDD24 pKa = 4.56ALTQHH29 pKa = 7.06LFRR32 pKa = 11.84RR33 pKa = 11.84GEE35 pKa = 4.21SYY37 pKa = 10.17TLVIMGVDD45 pKa = 3.31DD46 pKa = 3.76SGRR49 pKa = 11.84PISQSIWMAPTIPVQFLYY67 pKa = 10.94ADD69 pKa = 3.97YY70 pKa = 10.51EE71 pKa = 4.58SVEE74 pKa = 4.06IPRR77 pKa = 11.84DD78 pKa = 3.51DD79 pKa = 4.3FKK81 pKa = 11.98AMFDD85 pKa = 3.97DD86 pKa = 4.35VVEE89 pKa = 4.5SGMHH93 pKa = 5.35ICGSGPLAYY102 pKa = 10.08QFTGDD107 pKa = 3.59QPEE110 pKa = 4.84DD111 pKa = 3.6NPEE114 pKa = 3.79SS115 pKa = 3.64

Molecular weight: 12.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EFT1|A0A4Y6EFT1_9CAUD Helix-turn-helix DNA binding protein OS=Gordonia phage Twinkle OX=2583043 GN=74 PE=4 SV=1
MM1 pKa = 6.16TTLRR5 pKa = 11.84RR6 pKa = 11.84CTRR9 pKa = 11.84PGCEE13 pKa = 3.41KK14 pKa = 10.45RR15 pKa = 11.84PRR17 pKa = 11.84SRR19 pKa = 11.84GLCHH23 pKa = 5.62THH25 pKa = 6.78YY26 pKa = 9.14MDD28 pKa = 6.13HH29 pKa = 6.62ITRR32 pKa = 11.84QKK34 pKa = 11.18AYY36 pKa = 10.13GRR38 pKa = 11.84WEE40 pKa = 3.89SSLIDD45 pKa = 3.68AEE47 pKa = 4.26PTRR50 pKa = 11.84QHH52 pKa = 5.98VEE54 pKa = 3.61RR55 pKa = 11.84LRR57 pKa = 11.84AAGVGTRR64 pKa = 11.84KK65 pKa = 9.59ISEE68 pKa = 4.23TAGVTRR74 pKa = 11.84SVLQSLIHH82 pKa = 5.91GKK84 pKa = 7.21TRR86 pKa = 11.84NGHH89 pKa = 6.0RR90 pKa = 11.84EE91 pKa = 4.03PPSKK95 pKa = 9.88TIAKK99 pKa = 9.91RR100 pKa = 11.84IADD103 pKa = 4.63KK104 pKa = 10.77ILAVPVPAQPLRR116 pKa = 11.84NGDD119 pKa = 3.62VPALGTRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84LQALIAHH135 pKa = 7.62GYY137 pKa = 7.86SQAEE141 pKa = 3.93INRR144 pKa = 11.84RR145 pKa = 11.84FGLAADD151 pKa = 4.15NLAEE155 pKa = 5.02LIRR158 pKa = 11.84GEE160 pKa = 4.17RR161 pKa = 11.84NYY163 pKa = 10.51VDD165 pKa = 4.02PLTEE169 pKa = 3.8RR170 pKa = 11.84RR171 pKa = 11.84VTALFRR177 pKa = 11.84EE178 pKa = 4.6LQLTPGPCTRR188 pKa = 11.84ARR190 pKa = 11.84NRR192 pKa = 11.84GRR194 pKa = 11.84KK195 pKa = 9.03NGWPLPLDD203 pKa = 3.47WDD205 pKa = 4.06EE206 pKa = 6.36DD207 pKa = 4.67RR208 pKa = 11.84IDD210 pKa = 4.01DD211 pKa = 4.21PNYY214 pKa = 8.32EE215 pKa = 4.17AEE217 pKa = 4.15RR218 pKa = 11.84SHH220 pKa = 6.07VRR222 pKa = 11.84SIVRR226 pKa = 11.84DD227 pKa = 3.49PEE229 pKa = 4.53RR230 pKa = 11.84IEE232 pKa = 3.58WRR234 pKa = 11.84KK235 pKa = 9.75QKK237 pKa = 10.48VAEE240 pKa = 4.18LTALGYY246 pKa = 9.82SAEE249 pKa = 4.89DD250 pKa = 2.74IAARR254 pKa = 11.84LRR256 pKa = 11.84CTARR260 pKa = 11.84TVQRR264 pKa = 11.84DD265 pKa = 3.45RR266 pKa = 11.84SALGIVEE273 pKa = 4.47RR274 pKa = 11.84EE275 pKa = 4.06QVASS279 pKa = 3.61

Molecular weight: 31.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

17536

37

2535

222.0

24.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.81 ± 0.475

0.924 ± 0.143

6.826 ± 0.303

5.817 ± 0.306

2.458 ± 0.206

8.286 ± 0.429

2.161 ± 0.204

4.482 ± 0.183

3.205 ± 0.215

8.246 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.137

3.028 ± 0.232

5.446 ± 0.232

3.633 ± 0.289

7.641 ± 0.407

5.497 ± 0.24

6.9 ± 0.24

7.1 ± 0.226

2.064 ± 0.151

2.258 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski