Helicobacter hepaticus (strain ATCC 51449 / 3B1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; Helicobacter hepaticus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1872 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7VJH6|Q7VJH6_HELHP Uncharacterized protein OS=Helicobacter hepaticus (strain ATCC 51449 / 3B1) OX=235279 GN=HH_0267 PE=4 SV=1
MM1 pKa = 7.18IQLSLFEE8 pKa = 3.92EE9 pKa = 5.52AYY11 pKa = 9.97AEE13 pKa = 3.97EE14 pKa = 4.74MIRR17 pKa = 11.84ATEE20 pKa = 3.9YY21 pKa = 10.28GSLVKK26 pKa = 10.49IHH28 pKa = 5.84QDD30 pKa = 3.18KK31 pKa = 11.29VSGLYY36 pKa = 10.43LVTEE40 pKa = 4.14IEE42 pKa = 4.52EE43 pKa = 4.34GQEE46 pKa = 4.08DD47 pKa = 4.42YY48 pKa = 10.79TLSFEE53 pKa = 4.79TYY55 pKa = 10.69DD56 pKa = 3.39EE57 pKa = 4.17ALVYY61 pKa = 10.09FRR63 pKa = 11.84HH64 pKa = 6.57FIVDD68 pKa = 3.13INSNIEE74 pKa = 3.91LKK76 pKa = 10.51EE77 pKa = 4.42DD78 pKa = 3.64GSIGYY83 pKa = 9.42YY84 pKa = 10.13PEE86 pKa = 3.92ATQAEE91 pKa = 5.11DD92 pKa = 3.89YY93 pKa = 11.22SNQPTLFDD101 pKa = 3.25FTYY104 pKa = 11.06

Molecular weight: 12.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7VJX9|Q7VJX9_HELHP Uncharacterized protein OS=Helicobacter hepaticus (strain ATCC 51449 / 3B1) OX=235279 GN=HH_0113 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.32RR14 pKa = 11.84THH16 pKa = 6.11GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.44TKK25 pKa = 10.16NGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.41GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1872

0

1872

557311

30

2894

297.7

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.048 ± 0.054

1.358 ± 0.028

4.92 ± 0.04

6.455 ± 0.054

5.133 ± 0.055

5.987 ± 0.055

2.26 ± 0.028

8.566 ± 0.058

7.563 ± 0.053

10.287 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.024

5.281 ± 0.05

3.297 ± 0.036

4.008 ± 0.033

3.635 ± 0.034

6.98 ± 0.04

4.589 ± 0.035

5.452 ± 0.051

0.805 ± 0.02

3.952 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski