Austwickia chelonae NBRC 105200

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermatophilaceae; Austwickia; Austwickia chelonae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K6WAK8|K6WAK8_9MICO Uncharacterized protein OS=Austwickia chelonae NBRC 105200 OX=1184607 GN=AUCHE_17_00890 PE=4 SV=1
MM1 pKa = 6.96TVAPGSDD8 pKa = 4.07RR9 pKa = 11.84IPAVAAPEE17 pKa = 4.29CVGFEE22 pKa = 4.03AAQPAGLDD30 pKa = 3.27VLTPCRR36 pKa = 11.84DD37 pKa = 3.59HH38 pKa = 7.29EE39 pKa = 4.65SLPSIQDD46 pKa = 3.49PEE48 pKa = 4.31LVEE51 pKa = 3.82VDD53 pKa = 4.07PAVEE57 pKa = 4.26AQDD60 pKa = 3.91GAPEE64 pKa = 3.97VHH66 pKa = 6.88LCPTCLVPSGGDD78 pKa = 3.04ADD80 pKa = 4.4LRR82 pKa = 11.84PAVEE86 pKa = 4.49FPGVEE91 pKa = 3.77PRR93 pKa = 11.84LCPDD97 pKa = 3.64CEE99 pKa = 4.41VPSVDD104 pKa = 3.81YY105 pKa = 10.29ALSMAIEE112 pKa = 4.13EE113 pKa = 4.65LVDD116 pKa = 3.41EE117 pKa = 4.54AEE119 pKa = 4.16RR120 pKa = 11.84WDD122 pKa = 3.98VLDD125 pKa = 3.91AAVWFAPP132 pKa = 3.87

Molecular weight: 13.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K6VAH3|K6VAH3_9MICO Uncharacterized protein OS=Austwickia chelonae NBRC 105200 OX=1184607 GN=AUCHE_22_00080 PE=3 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.68THH17 pKa = 5.32GFRR20 pKa = 11.84KK21 pKa = 10.03RR22 pKa = 11.84MATRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.65GRR40 pKa = 11.84SEE42 pKa = 3.94LSAA45 pKa = 4.73

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3048

0

3048

1038619

45

4570

340.8

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.329 ± 0.058

0.921 ± 0.013

5.838 ± 0.036

5.575 ± 0.041

2.815 ± 0.026

9.133 ± 0.044

2.285 ± 0.026

3.971 ± 0.029

2.344 ± 0.041

9.994 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.019

1.881 ± 0.022

5.826 ± 0.039

3.012 ± 0.023

7.511 ± 0.04

5.791 ± 0.034

6.152 ± 0.041

9.067 ± 0.043

1.465 ± 0.016

1.981 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski