Actinobaculum sp. oral taxon 183 str. F0552

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinobaculum; unclassified Actinobaculum; Actinobaculum sp. oral taxon 183

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2320 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U1Q9J8|U1Q9J8_9ACTO Glucokinase OS=Actinobaculum sp. oral taxon 183 str. F0552 OX=1227261 GN=HMPREF0043_00961 PE=3 SV=1
MM1 pKa = 8.12DD2 pKa = 3.57GTFTVNTDD10 pKa = 3.21SLRR13 pKa = 11.84TAKK16 pKa = 8.7THH18 pKa = 6.21YY19 pKa = 10.53DD20 pKa = 3.46SASSGMYY27 pKa = 9.44NQEE30 pKa = 4.02SLTASGFGDD39 pKa = 3.75SQSWADD45 pKa = 3.92NVCSTLGTSLSDD57 pKa = 3.67LATKK61 pKa = 10.49ASQLASTLSTDD72 pKa = 3.92AEE74 pKa = 4.78CFDD77 pKa = 3.61STDD80 pKa = 3.89RR81 pKa = 11.84DD82 pKa = 3.91VQSDD86 pKa = 3.77IQCATSSDD94 pKa = 3.75HH95 pKa = 7.16

Molecular weight: 10.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U1R5K9|U1R5K9_9ACTO UDP-N-acetylmuramoylalanine--D-glutamate ligase OS=Actinobaculum sp. oral taxon 183 str. F0552 OX=1227261 GN=murD PE=3 SV=1
MM1 pKa = 7.2TGGRR5 pKa = 11.84GAGGVRR11 pKa = 11.84SGRR14 pKa = 11.84GLSRR18 pKa = 11.84PVAPRR23 pKa = 11.84LGRR26 pKa = 11.84RR27 pKa = 11.84GGRR30 pKa = 11.84AGAPSALGRR39 pKa = 11.84RR40 pKa = 4.12

Molecular weight: 3.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2320

0

2320

695025

37

2113

299.6

32.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.984 ± 0.084

0.856 ± 0.017

5.91 ± 0.047

5.875 ± 0.056

3.0 ± 0.034

9.409 ± 0.052

1.903 ± 0.022

4.215 ± 0.039

2.756 ± 0.05

9.319 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.078 ± 0.023

2.138 ± 0.032

5.411 ± 0.046

2.622 ± 0.029

7.988 ± 0.058

6.271 ± 0.049

5.374 ± 0.043

8.376 ± 0.053

1.365 ± 0.022

2.148 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski