Nocardioides sp. Leaf285

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3888 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S9QB85|A0A0S9QB85_9ACTN Uncharacterized protein OS=Nocardioides sp. Leaf285 OX=1736322 GN=ASF47_02535 PE=4 SV=1
MM1 pKa = 7.43LALVGAVAVVAAAVVVPILVTRR23 pKa = 11.84SEE25 pKa = 4.46EE26 pKa = 4.02PVEE29 pKa = 4.14QQATLAAIEE38 pKa = 4.23QYY40 pKa = 11.42ADD42 pKa = 3.2LRR44 pKa = 11.84RR45 pKa = 11.84DD46 pKa = 3.52HH47 pKa = 7.66VEE49 pKa = 3.82GSVDD53 pKa = 3.78YY54 pKa = 9.61PQSPPVGGPHH64 pKa = 5.23AQEE67 pKa = 4.05WLDD70 pKa = 3.52CGAYY74 pKa = 8.3DD75 pKa = 3.66TQVPAEE81 pKa = 4.25NLVHH85 pKa = 6.93DD86 pKa = 5.2LEE88 pKa = 6.5HH89 pKa = 5.91GTVVLTYY96 pKa = 10.57EE97 pKa = 4.64PGLPADD103 pKa = 4.75DD104 pKa = 3.89VAALADD110 pKa = 3.75VLPANGILSPWEE122 pKa = 3.88GQAAPVVVTVWGVQLEE138 pKa = 4.49LEE140 pKa = 4.6GADD143 pKa = 4.1DD144 pKa = 3.81PRR146 pKa = 11.84LAMFLQEE153 pKa = 4.37FGHH156 pKa = 6.72GEE158 pKa = 3.86TAPEE162 pKa = 4.48FGASCEE168 pKa = 4.2GGIDD172 pKa = 3.55PADD175 pKa = 3.59AAGAGTPVV183 pKa = 2.89

Molecular weight: 18.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S9Q191|A0A0S9Q191_9ACTN Alanine racemase OS=Nocardioides sp. Leaf285 OX=1736322 GN=ASF47_16695 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 4.97KK15 pKa = 10.04VHH17 pKa = 6.59GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILSSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.24GRR40 pKa = 11.84KK41 pKa = 8.84SLAVV45 pKa = 3.3

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3888

0

3888

1236740

37

3300

318.1

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.921 ± 0.06

0.714 ± 0.009

6.647 ± 0.035

5.813 ± 0.042

2.514 ± 0.023

9.478 ± 0.043

2.202 ± 0.02

2.683 ± 0.033

1.583 ± 0.028

10.393 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.695 ± 0.017

1.47 ± 0.019

5.922 ± 0.035

2.675 ± 0.023

7.975 ± 0.047

5.165 ± 0.029

6.088 ± 0.028

9.756 ± 0.045

1.499 ± 0.018

1.807 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski