Pelagibacterium luteolum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Devosiaceae; Pelagibacterium

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7S4M6|A0A1G7S4M6_9RHIZ Predicted aconitase subunit 1 OS=Pelagibacterium luteolum OX=440168 GN=SAMN04487974_101300 PE=4 SV=1
MM1 pKa = 7.39QEE3 pKa = 3.71FEE5 pKa = 4.26TTFRR9 pKa = 11.84QGVNGYY15 pKa = 10.42ASTVDD20 pKa = 3.64TQLLQANTGANHH32 pKa = 7.09ANTAVLGVDD41 pKa = 3.62RR42 pKa = 11.84NSHH45 pKa = 4.4VQVLLRR51 pKa = 11.84FDD53 pKa = 5.03DD54 pKa = 4.14LFGNAVSQVPAGAEE68 pKa = 3.82ILSATLTLHH77 pKa = 5.2TTNTGDD83 pKa = 3.23GATLHH88 pKa = 6.93RR89 pKa = 11.84MLTNWSGNSTWSSTGSGIQTNGVEE113 pKa = 4.32ALAAADD119 pKa = 3.9VNTGSTNLGASNFDD133 pKa = 3.24VTASVQAWAGGQANLGWVFVSLGTNGWDD161 pKa = 3.75FYY163 pKa = 10.78SAQGATPPQLTIRR176 pKa = 11.84YY177 pKa = 8.8RR178 pKa = 11.84IEE180 pKa = 4.37GEE182 pKa = 4.0PNTAPVANPDD192 pKa = 3.74TVVVDD197 pKa = 3.87EE198 pKa = 5.55DD199 pKa = 3.97GSATVNVLANDD210 pKa = 3.67ADD212 pKa = 4.26ADD214 pKa = 3.95GDD216 pKa = 4.17TLVVSGVGTPANGVAVLNGNGTITYY241 pKa = 7.59TPHH244 pKa = 7.33ANFFGNDD251 pKa = 3.22SFSYY255 pKa = 9.82TLSDD259 pKa = 3.41GKK261 pKa = 10.92GGQSTGQVSVTVNPVNDD278 pKa = 3.79APVATGDD285 pKa = 3.61TASVNEE291 pKa = 4.28GGNVVVTVLSNDD303 pKa = 3.08SDD305 pKa = 3.75IDD307 pKa = 3.68GDD309 pKa = 4.05ALSVSGVGTPSNGTAVINSNGTITYY334 pKa = 7.68TPNAGYY340 pKa = 8.33TGPDD344 pKa = 3.12SFSYY348 pKa = 10.07TINDD352 pKa = 3.8GKK354 pKa = 11.09GGTATASVAVTVHH367 pKa = 6.42PVNDD371 pKa = 3.74APVATGDD378 pKa = 3.75SVSVNEE384 pKa = 4.87DD385 pKa = 3.15GAVAIAVLANDD396 pKa = 4.48SDD398 pKa = 4.43PDD400 pKa = 4.13GDD402 pKa = 3.94VLTVTGVGTPAHH414 pKa = 6.41GLAIINSNGTITYY427 pKa = 7.75TPNANYY433 pKa = 9.95HH434 pKa = 6.08GPDD437 pKa = 3.08SFTYY441 pKa = 9.85TISDD445 pKa = 3.59GKK447 pKa = 10.7GGQATASVSVTVNSVNDD464 pKa = 3.68VPVADD469 pKa = 4.74ADD471 pKa = 3.76AASVIEE477 pKa = 4.51GGSVTVNVLSNDD489 pKa = 3.05SDD491 pKa = 4.31ADD493 pKa = 3.57GDD495 pKa = 4.13ALSVIGVGTPANGNAVLNGNGTITYY520 pKa = 7.65TPNAGYY526 pKa = 8.35TGPDD530 pKa = 3.2GFSYY534 pKa = 10.36TISDD538 pKa = 3.72GKK540 pKa = 10.82GGQATANVAITVNSDD555 pKa = 3.34TPNTVPVATSDD566 pKa = 3.73DD567 pKa = 3.72TSVLVDD573 pKa = 3.47GSVTVKK579 pKa = 10.51VLANDD584 pKa = 3.44YY585 pKa = 11.32DD586 pKa = 4.72LDD588 pKa = 4.84GDD590 pKa = 4.09SLAIISVSGPASGFAVVNPNGTITYY615 pKa = 7.62TPNAGYY621 pKa = 10.16SGPDD625 pKa = 2.93SFTYY629 pKa = 9.81TVADD633 pKa = 4.11GKK635 pKa = 11.24GGTAVGSVNVNVVEE649 pKa = 4.21PVSGDD654 pKa = 3.37VEE656 pKa = 4.33HH657 pKa = 6.77QVTFRR662 pKa = 11.84QGVNGYY668 pKa = 9.77SGTVDD673 pKa = 3.45TQILQSNTGANHH685 pKa = 6.95ANTAVIGVDD694 pKa = 2.78RR695 pKa = 11.84GTDD698 pKa = 3.41VQVLLRR704 pKa = 11.84FNDD707 pKa = 3.55LFGNGAFQVPPGSEE721 pKa = 3.71ILSATLTLQTTNTGNGATLHH741 pKa = 6.73RR742 pKa = 11.84MLTNWSDD749 pKa = 3.05TSTWASIVSGVQTNGVEE766 pKa = 4.14ALTAADD772 pKa = 3.64VRR774 pKa = 11.84TGATNLGASSYY785 pKa = 11.31DD786 pKa = 3.26VTASVQAWAGGQANLGWVFVSLGTNGWDD814 pKa = 3.77FYY816 pKa = 11.0SAQGSIPPQLTITYY830 pKa = 7.64RR831 pKa = 11.84APGGQEE837 pKa = 3.54NVAPTAAIDD846 pKa = 3.57AGVVNEE852 pKa = 5.01DD853 pKa = 3.25GSVVINPLANDD864 pKa = 3.79TDD866 pKa = 4.53PDD868 pKa = 4.09GGTLTIVGIGTPTNGVAVLNSNGTVTYY895 pKa = 9.58TPNANYY901 pKa = 10.19NGPDD905 pKa = 3.3SFSYY909 pKa = 10.21TISDD913 pKa = 3.94GQGGTASGTITIVVKK928 pKa = 9.72PVNDD932 pKa = 3.69APVAAGDD939 pKa = 4.03GAVVTQGGLVVVDD952 pKa = 4.32VLANDD957 pKa = 3.74SDD959 pKa = 4.12VDD961 pKa = 3.77GDD963 pKa = 4.05ALSVIGVGTPAHH975 pKa = 7.05GIAVINSDD983 pKa = 3.37GTVTYY988 pKa = 9.28TPAGGYY994 pKa = 9.52SGSDD998 pKa = 3.07SFSYY1002 pKa = 10.22TISDD1006 pKa = 3.67GKK1008 pKa = 10.88GGEE1011 pKa = 4.32ATGTAAVTVNASVPVNTPPVANPDD1035 pKa = 4.07SISVNEE1041 pKa = 4.79DD1042 pKa = 2.78GSVVISVLANDD1053 pKa = 3.84TDD1055 pKa = 4.66ANGHH1059 pKa = 6.3ALTVTGVGTPGHH1071 pKa = 5.59GTVVINGNGTITYY1084 pKa = 7.9TPTANYY1090 pKa = 9.12HH1091 pKa = 5.99GPDD1094 pKa = 3.24SFTYY1098 pKa = 9.83TISDD1102 pKa = 3.53GHH1104 pKa = 6.91GGAATGAVSITVNPVNDD1121 pKa = 3.84APVANADD1128 pKa = 4.08TIAVNEE1134 pKa = 4.24DD1135 pKa = 3.11AAVVISVLANDD1146 pKa = 3.95TDD1148 pKa = 3.8IDD1150 pKa = 4.48GDD1152 pKa = 4.03TLTVIGVGAPAHH1164 pKa = 6.16GSAVVNSNGTITYY1177 pKa = 7.89TPSTNYY1183 pKa = 10.39NGADD1187 pKa = 3.12SFTYY1191 pKa = 9.9TISDD1195 pKa = 3.59GKK1197 pKa = 10.7GGQATGTVNVTVNPINDD1214 pKa = 3.65APVANADD1221 pKa = 3.65SAFVQVGASVVVPVLLNDD1239 pKa = 3.24VDD1241 pKa = 3.93VDD1243 pKa = 3.86GDD1245 pKa = 3.95ALTVTGFAVAPQHH1258 pKa = 5.3GTAVVNGNGTVTYY1271 pKa = 8.89TPSAGYY1277 pKa = 10.35VGVDD1281 pKa = 2.79AFIYY1285 pKa = 9.28TISDD1289 pKa = 3.45GKK1291 pKa = 10.79GGSASATVTVNVTEE1305 pKa = 4.33QPDD1308 pKa = 3.99DD1309 pKa = 4.19PLHH1312 pKa = 6.49ASYY1315 pKa = 10.66IATAISGSTSARR1327 pKa = 11.84HH1328 pKa = 5.96LEE1330 pKa = 4.13HH1331 pKa = 7.15SNSTKK1336 pKa = 10.44SFYY1339 pKa = 10.63FAGAWWAILPNSANWSIHH1357 pKa = 5.35KK1358 pKa = 10.58FNGTTPDD1365 pKa = 3.36EE1366 pKa = 4.55GQLGGWTIKK1375 pKa = 9.47STALFASNSSNRR1387 pKa = 11.84ADD1389 pKa = 4.15LAWDD1393 pKa = 3.53PVTEE1397 pKa = 4.13TLYY1400 pKa = 11.37VMQYY1404 pKa = 11.12NNGTPRR1410 pKa = 11.84LFALDD1415 pKa = 3.72YY1416 pKa = 11.25AAGTDD1421 pKa = 3.33SWVVGSNIVLAGSGGVLSGSQWTSNIEE1448 pKa = 3.69MRR1450 pKa = 11.84LSIDD1454 pKa = 3.38QNGVPVVSAIGASGSSAGLHH1474 pKa = 6.26LGFATSSNLSTWGQVLLDD1492 pKa = 3.69ATTDD1496 pKa = 3.28RR1497 pKa = 11.84SGGSNGDD1504 pKa = 3.42SKK1506 pKa = 11.93ADD1508 pKa = 2.98IVFFTQGGANKK1519 pKa = 9.86IAVIYY1524 pKa = 10.56SRR1526 pKa = 11.84DD1527 pKa = 3.52GAGSDD1532 pKa = 3.93NDD1534 pKa = 3.62WSIVWRR1540 pKa = 11.84DD1541 pKa = 3.86TPQSAADD1548 pKa = 4.23YY1549 pKa = 10.08VNGWQKK1555 pKa = 10.75EE1556 pKa = 4.4VITDD1560 pKa = 3.52DD1561 pKa = 4.03VNIDD1565 pKa = 2.91NHH1567 pKa = 5.6MAAVSDD1573 pKa = 3.63GTTIYY1578 pKa = 11.12AVIKK1582 pKa = 10.83DD1583 pKa = 4.41DD1584 pKa = 6.67DD1585 pKa = 4.05NSLWLLKK1592 pKa = 9.82GHH1594 pKa = 7.08PGNWDD1599 pKa = 3.28APLLIVEE1606 pKa = 5.49GGAHH1610 pKa = 6.63DD1611 pKa = 4.07PSRR1614 pKa = 11.84PGIVLDD1620 pKa = 3.73HH1621 pKa = 6.33TNDD1624 pKa = 3.29RR1625 pKa = 11.84LFIYY1629 pKa = 9.62YY1630 pKa = 9.53QEE1632 pKa = 4.35STSDD1636 pKa = 3.42PEE1638 pKa = 4.12GAVFLKK1644 pKa = 9.94VTDD1647 pKa = 4.1ADD1649 pKa = 3.85NPTFDD1654 pKa = 4.23SDD1656 pKa = 4.87DD1657 pKa = 3.45IGMMILQGTNASHH1670 pKa = 7.81DD1671 pKa = 3.69MLDD1674 pKa = 3.74PQRR1677 pKa = 11.84PAHH1680 pKa = 6.78AVGTDD1685 pKa = 3.06TGNEE1689 pKa = 4.14FIILAKK1695 pKa = 10.2NQQTNTIWYY1704 pKa = 9.12NDD1706 pKa = 2.96VDD1708 pKa = 4.5LGFEE1712 pKa = 3.99YY1713 pKa = 10.68SIAA1716 pKa = 3.67

Molecular weight: 174.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7VAS4|A0A1G7VAS4_9RHIZ Antirestriction protein OS=Pelagibacterium luteolum OX=440168 GN=SAMN04487974_10423 PE=4 SV=1
MM1 pKa = 7.17SRR3 pKa = 11.84CRR5 pKa = 11.84GQSAVSGAPSLFEE18 pKa = 3.87TANTICRR25 pKa = 11.84KK26 pKa = 9.19FALSDD31 pKa = 3.32RR32 pKa = 11.84TVQRR36 pKa = 11.84MRR38 pKa = 11.84AANDD42 pKa = 3.02EE43 pKa = 4.44HH44 pKa = 9.15ALFTDD49 pKa = 3.88DD50 pKa = 5.43DD51 pKa = 4.2AANTEE56 pKa = 4.25VQLGHH61 pKa = 6.71TGGQVGVEE69 pKa = 4.47DD70 pKa = 5.15SSDD73 pKa = 3.56DD74 pKa = 4.9HH75 pKa = 8.77IGTAQKK81 pKa = 9.18NTINGPYY88 pKa = 9.91RR89 pKa = 11.84SIRR92 pKa = 11.84PLGRR96 pKa = 11.84FSTRR100 pKa = 11.84QWAWRR105 pKa = 11.84STSLKK110 pKa = 9.2TDD112 pKa = 3.73PSSCSAGRR120 pKa = 11.84ASSCTNTAAEE130 pKa = 4.24KK131 pKa = 9.26LTYY134 pKa = 10.6SRR136 pKa = 11.84VAPPLPRR143 pKa = 11.84RR144 pKa = 11.84AQAAIRR150 pKa = 11.84SAKK153 pKa = 9.73PRR155 pKa = 11.84VLRR158 pKa = 11.84TASMKK163 pKa = 10.56AFSRR167 pKa = 11.84SSRR170 pKa = 11.84SASFAMAA177 pKa = 4.46

Molecular weight: 19.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4253

0

4253

1271227

39

1837

298.9

32.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.1 ± 0.052

0.717 ± 0.01

5.996 ± 0.037

5.889 ± 0.041

3.841 ± 0.025

8.36 ± 0.038

2.022 ± 0.019

5.785 ± 0.029

3.063 ± 0.034

9.966 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.017

2.751 ± 0.022

4.963 ± 0.03

3.21 ± 0.024

6.641 ± 0.037

5.597 ± 0.026

5.562 ± 0.026

7.386 ± 0.03

1.311 ± 0.016

2.24 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski