Jingmen tombus-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG50|A0A1L3KG50_9VIRU RNA-directed RNA polymerase OS=Jingmen tombus-like virus 2 OX=1938656 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84DD3 pKa = 3.6YY4 pKa = 11.05EE5 pKa = 4.36ISNHH9 pKa = 5.96LNLPATRR16 pKa = 11.84PIKK19 pKa = 9.99WRR21 pKa = 11.84GQVLDD26 pKa = 5.15DD27 pKa = 4.55RR28 pKa = 11.84DD29 pKa = 3.74HH30 pKa = 6.69PAIIRR35 pKa = 11.84IGPSSGPQPYY45 pKa = 10.07APHH48 pKa = 6.84HH49 pKa = 6.02CWKK52 pKa = 10.09NWVRR56 pKa = 11.84GVKK59 pKa = 9.56KK60 pKa = 9.71RR61 pKa = 11.84CAHH64 pKa = 6.77AFHH67 pKa = 6.9PHH69 pKa = 6.97DD70 pKa = 4.19DD71 pKa = 3.68FEE73 pKa = 4.88RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84FFTFVQSQIKK86 pKa = 8.57TMPRR90 pKa = 11.84LRR92 pKa = 11.84SGLKK96 pKa = 10.18YY97 pKa = 10.64EE98 pKa = 4.6SLLDD102 pKa = 3.45DD103 pKa = 4.24WLAHH107 pKa = 5.89SNYY110 pKa = 8.5TAARR114 pKa = 11.84AAEE117 pKa = 4.14LKK119 pKa = 10.58KK120 pKa = 10.71LASDD124 pKa = 3.94YY125 pKa = 10.9MNGDD129 pKa = 2.59IDD131 pKa = 3.62MNRR134 pKa = 11.84IYY136 pKa = 10.96LCDD139 pKa = 3.62SFIKK143 pKa = 10.55SEE145 pKa = 4.15MYY147 pKa = 10.74DD148 pKa = 3.52EE149 pKa = 4.49IKK151 pKa = 9.93EE152 pKa = 3.93ARR154 pKa = 11.84IINARR159 pKa = 11.84TDD161 pKa = 3.13WFKK164 pKa = 11.54VIAGPYY170 pKa = 7.51IHH172 pKa = 7.3AIEE175 pKa = 4.21KK176 pKa = 10.23LVYY179 pKa = 9.73DD180 pKa = 3.86DD181 pKa = 5.66HH182 pKa = 6.61FVKK185 pKa = 10.62HH186 pKa = 5.45KK187 pKa = 9.64TPAQIVKK194 pKa = 10.12RR195 pKa = 11.84LNKK198 pKa = 9.51LRR200 pKa = 11.84SVGSIFMEE208 pKa = 3.91TDD210 pKa = 3.05YY211 pKa = 11.78SSFEE215 pKa = 3.98GSFDD219 pKa = 3.93PEE221 pKa = 4.15LQRR224 pKa = 11.84HH225 pKa = 5.05VEE227 pKa = 3.9RR228 pKa = 11.84ALWDD232 pKa = 3.58HH233 pKa = 6.28MLVDD237 pKa = 4.08YY238 pKa = 10.82PEE240 pKa = 4.37ILSIIQRR247 pKa = 11.84AYY249 pKa = 9.16SRR251 pKa = 11.84SHH253 pKa = 5.68IRR255 pKa = 11.84YY256 pKa = 8.91KK257 pKa = 10.88NRR259 pKa = 11.84AHH261 pKa = 7.39AIFEE265 pKa = 4.2GSRR268 pKa = 11.84MSGDD272 pKa = 2.85MWTSLANGFTNKK284 pKa = 9.43MINEE288 pKa = 4.0YY289 pKa = 9.83MAMCARR295 pKa = 11.84DD296 pKa = 3.48NEE298 pKa = 3.93YY299 pKa = 10.95DD300 pKa = 3.88YY301 pKa = 11.61LVEE304 pKa = 6.1GDD306 pKa = 4.37DD307 pKa = 6.03AIICSSSRR315 pKa = 11.84WDD317 pKa = 3.24TDD319 pKa = 2.75IPARR323 pKa = 11.84LGFKK327 pKa = 9.5LTLEE331 pKa = 4.24TASDD335 pKa = 3.9FNEE338 pKa = 4.61LSFCGLNAVDD348 pKa = 5.51GVLIPNVRR356 pKa = 11.84KK357 pKa = 9.49ILTRR361 pKa = 11.84YY362 pKa = 10.21GYY364 pKa = 7.19TAEE367 pKa = 3.99MKK369 pKa = 10.69YY370 pKa = 11.31YY371 pKa = 10.15MDD373 pKa = 3.78VKK375 pKa = 10.26TNNQRR380 pKa = 11.84YY381 pKa = 8.8RR382 pKa = 11.84DD383 pKa = 3.78LMYY386 pKa = 11.08SKK388 pKa = 10.83ACSLLATSAKK398 pKa = 10.14VPVLGEE404 pKa = 3.94LARR407 pKa = 11.84QQMRR411 pKa = 11.84VWNGVNNFRR420 pKa = 11.84YY421 pKa = 8.83YY422 pKa = 10.98DD423 pKa = 2.72WWEE426 pKa = 3.89RR427 pKa = 11.84QFYY430 pKa = 10.47SYY432 pKa = 11.25DD433 pKa = 3.53EE434 pKa = 4.57PEE436 pKa = 4.56PLQSQPISMSVRR448 pKa = 11.84RR449 pKa = 11.84FVEE452 pKa = 4.31EE453 pKa = 4.78KK454 pKa = 8.54YY455 pKa = 10.56HH456 pKa = 7.89IPIVLQLQLEE466 pKa = 4.33EE467 pKa = 5.28EE468 pKa = 4.2IRR470 pKa = 11.84CCNFKK475 pKa = 11.05VFDD478 pKa = 3.84IEE480 pKa = 4.21FF481 pKa = 4.1

Molecular weight: 56.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG11|A0A1L3KG11_9VIRU Uncharacterized protein OS=Jingmen tombus-like virus 2 OX=1938656 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84KK3 pKa = 9.97ANLMNGASGPTIQSNNNNPPKK24 pKa = 10.76SNNDD28 pKa = 3.12ANRR31 pKa = 11.84VVPASRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84NRR43 pKa = 11.84NRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84APFTRR53 pKa = 11.84PSPPLAEE60 pKa = 4.12SASLNTYY67 pKa = 10.71ADD69 pKa = 3.16IVQRR73 pKa = 11.84GGMAILRR80 pKa = 11.84CRR82 pKa = 11.84EE83 pKa = 3.95VFQILAPLSEE93 pKa = 4.46SPIALMLPATPTKK106 pKa = 8.24WTRR109 pKa = 11.84TRR111 pKa = 11.84TGVLSSTYY119 pKa = 10.24AAYY122 pKa = 10.25RR123 pKa = 11.84PLQMRR128 pKa = 11.84LTYY131 pKa = 9.95VPSVAATTAGSVVVGTVFAGVRR153 pKa = 11.84LEE155 pKa = 4.15TTDD158 pKa = 3.77FNGLIRR164 pKa = 11.84QMPVSNGGFVTTVWHH179 pKa = 6.26SCTSRR184 pKa = 11.84VSLGTNLRR192 pKa = 11.84ANNFPTFSVDD202 pKa = 2.93MDD204 pKa = 5.97DD205 pKa = 3.38IPFWILVGTSSTVQPGWLVVEE226 pKa = 4.72AQFSLHH232 pKa = 6.56NPLNNQGAVSFSGFGPATFTQGTGNTTISIPQADD266 pKa = 3.8FVGDD270 pKa = 3.91LEE272 pKa = 4.45SGRR275 pKa = 11.84EE276 pKa = 4.02YY277 pKa = 11.28VFTPSASVGPSEE289 pKa = 4.53GVVAGTLEE297 pKa = 4.33PFRR300 pKa = 11.84AAYY303 pKa = 7.7TALTGDD309 pKa = 3.82NYY311 pKa = 10.84QFIGEE316 pKa = 4.05FSIPSTVTQFIAGVGRR332 pKa = 11.84SANFTQVEE340 pKa = 4.21

Molecular weight: 36.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1051

230

481

350.3

39.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.517 ± 0.496

1.427 ± 0.302

5.233 ± 1.015

4.757 ± 0.758

4.948 ± 0.234

5.614 ± 0.981

2.188 ± 0.629

5.709 ± 0.697

3.33 ± 1.081

7.612 ± 0.637

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.854 ± 0.353

5.138 ± 0.607

5.519 ± 0.738

3.52 ± 0.211

7.802 ± 0.271

8.088 ± 0.635

7.041 ± 1.937

6.185 ± 0.95

1.522 ± 0.443

3.996 ± 0.845
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski