Hubei diptera virus 14

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEM7|A0A1L3KEM7_9VIRU Uncharacterized protein OS=Hubei diptera virus 14 OX=1922875 PE=4 SV=1
MM1 pKa = 7.33FNSFEE6 pKa = 4.26YY7 pKa = 8.77PTIVVNDD14 pKa = 3.85FNLLNGQFDD23 pKa = 4.58DD24 pKa = 4.89PPLGANMTYY33 pKa = 9.33QAPMAVASDD42 pKa = 3.48QSTIGGSRR50 pKa = 11.84IKK52 pKa = 10.7PEE54 pKa = 4.05VNFRR58 pKa = 11.84TPRR61 pKa = 11.84EE62 pKa = 3.42IWQGFEE68 pKa = 4.04RR69 pKa = 11.84NEE71 pKa = 3.91DD72 pKa = 3.53FYY74 pKa = 11.28EE75 pKa = 3.71IHH77 pKa = 6.4LQFGLFGPFSEE88 pKa = 5.16QQGADD93 pKa = 3.57PNIKK97 pKa = 10.08QGTLKK102 pKa = 10.07GTFEE106 pKa = 4.27VDD108 pKa = 2.92PKK110 pKa = 10.77EE111 pKa = 3.69FDD113 pKa = 3.48IIKK116 pKa = 10.15FMPGTFDD123 pKa = 3.6TFTTSNFGDD132 pKa = 3.61NAVATMMVLVRR143 pKa = 11.84KK144 pKa = 9.32LVIGIQFRR152 pKa = 11.84LPVFKK157 pKa = 9.93FTFEE161 pKa = 4.43LNRR164 pKa = 11.84DD165 pKa = 3.15IPINEE170 pKa = 4.57PYY172 pKa = 10.68NIILMGSWFLMRR184 pKa = 11.84HH185 pKa = 5.17GVRR188 pKa = 11.84SFRR191 pKa = 11.84SIEE194 pKa = 3.98DD195 pKa = 3.19QATVEE200 pKa = 4.17EE201 pKa = 4.82EE202 pKa = 3.85IEE204 pKa = 4.14EE205 pKa = 4.2

Molecular weight: 23.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEK4|A0A1L3KEK4_9VIRU Uncharacterized protein OS=Hubei diptera virus 14 OX=1922875 PE=4 SV=1
MM1 pKa = 6.86KK2 pKa = 9.29TCPVCQRR9 pKa = 11.84KK10 pKa = 7.94FVNKK14 pKa = 9.5QALKK18 pKa = 9.66QHH20 pKa = 6.46MDD22 pKa = 3.33SAHH25 pKa = 6.41GKK27 pKa = 7.06PAPVRR32 pKa = 11.84RR33 pKa = 11.84TKK35 pKa = 11.34NNINNMIVSRR45 pKa = 11.84GNDD48 pKa = 2.82NDD50 pKa = 3.06IPIRR54 pKa = 11.84IKK56 pKa = 10.5RR57 pKa = 11.84KK58 pKa = 9.35EE59 pKa = 4.08FIQAIDD65 pKa = 3.5KK66 pKa = 10.19TNLNGNLVFNPRR78 pKa = 11.84TAASSKK84 pKa = 10.19ILNKK88 pKa = 9.58FAKK91 pKa = 10.03IYY93 pKa = 10.63DD94 pKa = 3.6NYY96 pKa = 9.94IVHH99 pKa = 6.15SCSVQFVSGSRR110 pKa = 11.84STRR113 pKa = 11.84NGMVVVAVDD122 pKa = 3.79YY123 pKa = 10.78GSSSIVTNTKK133 pKa = 9.87EE134 pKa = 3.84NLYY137 pKa = 10.3ALPSVATQVHH147 pKa = 6.32SNSPVLKK154 pKa = 9.78ISVSNAVRR162 pKa = 11.84YY163 pKa = 10.49CNVTDD168 pKa = 4.2KK169 pKa = 11.39NRR171 pKa = 11.84DD172 pKa = 3.54NPFTIYY178 pKa = 9.76WYY180 pKa = 10.73CDD182 pKa = 3.07TTEE185 pKa = 4.01TDD187 pKa = 3.48GGVGDD192 pKa = 5.56LFLTYY197 pKa = 10.35DD198 pKa = 3.65IEE200 pKa = 4.98FKK202 pKa = 11.17GLTPP206 pKa = 4.75

Molecular weight: 23.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1226

205

434

306.5

34.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.894 ± 0.615

1.958 ± 0.53

5.139 ± 0.422

5.791 ± 1.132

4.405 ± 1.595

7.586 ± 1.301

1.794 ± 0.182

6.281 ± 0.458

6.852 ± 0.799

7.586 ± 0.885

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.855 ± 0.311

6.199 ± 1.056

4.241 ± 0.49

3.67 ± 0.29

4.078 ± 0.392

7.259 ± 0.805

5.628 ± 0.471

7.667 ± 0.945

1.713 ± 0.791

4.405 ± 0.803
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski