Halopolyspora algeriensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetia incertae sedis; Halopolyspora

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A368VMZ0|A0A368VMZ0_9ACTN Succinate dehydrogenase / fumarate reductase iron-sulfur subunit OS=Halopolyspora algeriensis OX=1500506 GN=DFQ14_108140 PE=3 SV=1
MM1 pKa = 7.37LHH3 pKa = 6.13SQSEE7 pKa = 4.05QDD9 pKa = 3.53PGSMHH14 pKa = 6.01STSAGAPTSNSGTQAAQADD33 pKa = 4.23QPTLYY38 pKa = 10.6EE39 pKa = 4.57FLTQLVSNPQARR51 pKa = 11.84SAFEE55 pKa = 3.99ADD57 pKa = 3.35PRR59 pKa = 11.84ASLDD63 pKa = 3.55GAGLGDD69 pKa = 3.69MTATDD74 pKa = 4.01VLQGASLVLDD84 pKa = 3.58YY85 pKa = 11.42APAEE89 pKa = 4.3VVQEE93 pKa = 4.15YY94 pKa = 10.75GRR96 pKa = 11.84SLQSSVDD103 pKa = 3.35TFAASTQHH111 pKa = 5.79VAINQLHH118 pKa = 6.74PAQEE122 pKa = 4.57HH123 pKa = 4.6EE124 pKa = 4.4HH125 pKa = 6.46QEE127 pKa = 3.92ATEE130 pKa = 4.11PSMLQNSQDD139 pKa = 3.3TSSNGDD145 pKa = 3.34ADD147 pKa = 4.03EE148 pKa = 5.61QMPADD153 pKa = 3.97NGNNGGGNAEE163 pKa = 4.13AGNNVEE169 pKa = 4.29VNHH172 pKa = 6.09SVEE175 pKa = 4.4HH176 pKa = 6.15TDD178 pKa = 2.87SHH180 pKa = 6.99NVVSVHH186 pKa = 7.24DD187 pKa = 4.13VLSDD191 pKa = 3.37NSLAGVGNTVDD202 pKa = 3.96SVGDD206 pKa = 3.74TVNNTVNSVSSTVDD220 pKa = 2.91AVGVNNVVGGVANVAVEE237 pKa = 4.2DD238 pKa = 3.8VVGNVTGAVGGVVGGVTGAGDD259 pKa = 3.53VQTYY263 pKa = 10.69GGDD266 pKa = 3.57AEE268 pKa = 4.89ANAGLVGNVGGTVDD282 pKa = 4.27GVTSGLGLDD291 pKa = 3.87VLL293 pKa = 4.64

Molecular weight: 29.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368W544|A0A368W544_9ACTN Putative integral membrane protein OS=Halopolyspora algeriensis OX=1500506 GN=DFQ14_101515 PE=4 SV=1
MM1 pKa = 7.47SKK3 pKa = 10.58GKK5 pKa = 8.48RR6 pKa = 11.84TYY8 pKa = 10.26QPNNRR13 pKa = 11.84KK14 pKa = 8.98RR15 pKa = 11.84AKK17 pKa = 8.46THH19 pKa = 5.11GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVAARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84GRR42 pKa = 11.84KK43 pKa = 8.69QLTAA47 pKa = 3.98

Molecular weight: 5.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4304

0

4304

1331474

26

2073

309.4

33.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.355 ± 0.049

0.833 ± 0.011

5.866 ± 0.035

6.36 ± 0.041

2.778 ± 0.022

9.209 ± 0.038

2.511 ± 0.018

3.48 ± 0.025

1.85 ± 0.023

10.21 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.947 ± 0.015

1.921 ± 0.019

5.734 ± 0.026

3.153 ± 0.026

8.112 ± 0.043

5.675 ± 0.022

6.024 ± 0.026

8.586 ± 0.032

1.467 ± 0.017

1.929 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski