Vibrio phage ICP3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Chatterjeevirus; Vibrio virus ICP3

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F1D046|F1D046_9CAUD DNA packaging/maturation protein A OS=Vibrio phage ICP3 OX=979535 GN=18 PE=4 SV=1
MM1 pKa = 7.1PTPRR5 pKa = 11.84SLNTVEE11 pKa = 5.03FGWNGADD18 pKa = 2.79GWVIEE23 pKa = 4.59KK24 pKa = 10.72LSGEE28 pKa = 4.09IGVEE32 pKa = 4.15TVADD36 pKa = 3.81NDD38 pKa = 3.86VLISNSTPEE47 pKa = 3.7YY48 pKa = 8.96WGLHH52 pKa = 4.65KK53 pKa = 10.76VISEE57 pKa = 3.92IDD59 pKa = 3.89LKK61 pKa = 11.31NLLHH65 pKa = 6.59VPVNLEE71 pKa = 3.58GTFADD76 pKa = 3.62MAGGYY81 pKa = 7.94NLCLLAYY88 pKa = 10.27NGATEE93 pKa = 4.11WDD95 pKa = 3.49NTQAIITNFANDD107 pKa = 3.34NPIYY111 pKa = 10.37ADD113 pKa = 2.91NWTEE117 pKa = 3.88VQTAFIPEE125 pKa = 4.52GGTSASLTFNLTNTYY140 pKa = 10.77KK141 pKa = 10.84NLAVMLL147 pKa = 4.41

Molecular weight: 16.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1D017|F1D017_9CAUD Uncharacterized protein orf21 OS=Vibrio phage ICP3 OX=979535 GN=orf21 PE=4 SV=1
MM1 pKa = 7.79TITPDD6 pKa = 3.97LLRR9 pKa = 11.84KK10 pKa = 9.45LAAKK14 pKa = 10.21LDD16 pKa = 3.76AAQAEE21 pKa = 4.7VANEE25 pKa = 3.97LKK27 pKa = 10.43EE28 pKa = 3.95ALGMCEE34 pKa = 3.91QPVQRR39 pKa = 11.84TTFQLVGKK47 pKa = 8.41PVSKK51 pKa = 10.43AVQAKK56 pKa = 9.23RR57 pKa = 11.84VEE59 pKa = 4.22RR60 pKa = 11.84EE61 pKa = 3.51MKK63 pKa = 10.1RR64 pKa = 11.84WVEE67 pKa = 3.79ANMTDD72 pKa = 4.25EE73 pKa = 4.05EE74 pKa = 4.4RR75 pKa = 11.84TRR77 pKa = 11.84QARR80 pKa = 11.84RR81 pKa = 11.84KK82 pKa = 9.11KK83 pKa = 7.81ATKK86 pKa = 10.19GVTHH90 pKa = 7.86RR91 pKa = 11.84ITKK94 pKa = 9.28EE95 pKa = 3.66INHH98 pKa = 6.49PRR100 pKa = 11.84YY101 pKa = 9.19EE102 pKa = 4.29HH103 pKa = 6.13LRR105 pKa = 11.84KK106 pKa = 10.05AGFCAGMKK114 pKa = 9.84FSIVSRR120 pKa = 11.84HH121 pKa = 4.22SLNRR125 pKa = 11.84VVVEE129 pKa = 4.93HH130 pKa = 6.39IRR132 pKa = 11.84SGKK135 pKa = 8.49RR136 pKa = 11.84AIMSTTLLEE145 pKa = 4.59TIPDD149 pKa = 3.82GQFF152 pKa = 2.73

Molecular weight: 17.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11945

25

1127

221.2

24.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.648 ± 0.476

1.088 ± 0.171

5.802 ± 0.255

7.468 ± 0.356

4.177 ± 0.216

6.865 ± 0.408

1.75 ± 0.191

5.458 ± 0.161

6.873 ± 0.349

8.221 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.014 ± 0.167

4.722 ± 0.231

3.55 ± 0.169

4.186 ± 0.3

4.83 ± 0.18

5.768 ± 0.294

5.818 ± 0.32

6.781 ± 0.261

1.373 ± 0.173

3.608 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski