Pusillimonas sp. T2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Pusillimonas; unclassified Pusillimonas

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A231HKR1|A0A231HKR1_9BURK ABC transporter substrate-binding protein OS=Pusillimonas sp. T2 OX=1548123 GN=PuT2_06620 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.24VSSQIVSALPQGLILDD18 pKa = 4.5VIIEE22 pKa = 4.52DD23 pKa = 3.95LTFTAYY29 pKa = 10.5VVVSAPDD36 pKa = 3.07IHH38 pKa = 7.79LVAEE42 pKa = 5.0FVSPEE47 pKa = 3.92AYY49 pKa = 9.62QQANDD54 pKa = 3.24WHH56 pKa = 5.64VTAIEE61 pKa = 4.14TADD64 pKa = 3.58DD65 pKa = 3.98AEE67 pKa = 4.28NQVRR71 pKa = 11.84DD72 pKa = 3.23IAFNMNLNDD81 pKa = 4.0AAVFLCADD89 pKa = 4.17TAAYY93 pKa = 7.89EE94 pKa = 4.24AALEE98 pKa = 4.2ALGQTPLAGG107 pKa = 3.55

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A231HH61|A0A231HH61_9BURK Carbon monoxide dehydrogenase OS=Pusillimonas sp. T2 OX=1548123 GN=PuT2_13910 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.43RR14 pKa = 11.84THH16 pKa = 5.88GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3054

0

3054

1004463

34

5256

328.9

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.293 ± 0.05

0.926 ± 0.015

5.465 ± 0.032

5.216 ± 0.035

3.644 ± 0.03

7.856 ± 0.041

2.275 ± 0.025

5.114 ± 0.032

3.579 ± 0.035

10.574 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.503 ± 0.024

3.16 ± 0.028

5.0 ± 0.033

4.333 ± 0.033

6.235 ± 0.037

5.713 ± 0.035

5.431 ± 0.056

7.747 ± 0.037

1.426 ± 0.019

2.511 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski