Grapevine virus I

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8JK75|A0A2K8JK75_9VIRU Nucleic acid binding protein OS=Grapevine virus I OX=2052157 PE=4 SV=1
MM1 pKa = 7.08MNEE4 pKa = 4.56DD5 pKa = 3.37EE6 pKa = 4.56VATYY10 pKa = 9.93LANRR14 pKa = 11.84LGSQCSLEE22 pKa = 5.06SVCEE26 pKa = 4.23WYY28 pKa = 10.77NRR30 pKa = 11.84WLLDD34 pKa = 3.61AVHH37 pKa = 7.08PPLGFYY43 pKa = 10.61VVVSFFGDD51 pKa = 3.38EE52 pKa = 4.27SVSEE56 pKa = 3.93NLINYY61 pKa = 8.4LNLLARR67 pKa = 11.84VQGKK71 pKa = 8.65EE72 pKa = 3.71LVRR75 pKa = 11.84FTTQQVQRR83 pKa = 11.84SEE85 pKa = 4.41SKK87 pKa = 10.34FYY89 pKa = 10.68SANIRR94 pKa = 11.84LVKK97 pKa = 10.35EE98 pKa = 3.67LFITNNFSNPNLVEE112 pKa = 4.56FYY114 pKa = 10.86KK115 pKa = 11.21LLGSCYY121 pKa = 10.16LDD123 pKa = 2.81SCYY126 pKa = 10.57ISTGLGGTTLKK137 pKa = 10.94SSTLNSIKK145 pKa = 10.53LDD147 pKa = 3.6LTLDD151 pKa = 4.39DD152 pKa = 4.05IKK154 pKa = 11.41GVLKK158 pKa = 10.81RR159 pKa = 11.84LTGTVAVII167 pKa = 3.97

Molecular weight: 18.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8JK75|A0A2K8JK75_9VIRU Nucleic acid binding protein OS=Grapevine virus I OX=2052157 PE=4 SV=1
MM1 pKa = 7.64SGLLNSRR8 pKa = 11.84FLGEE12 pKa = 4.02SRR14 pKa = 11.84SAAKK18 pKa = 10.05RR19 pKa = 11.84RR20 pKa = 11.84AKK22 pKa = 10.32RR23 pKa = 11.84YY24 pKa = 8.64GRR26 pKa = 11.84CFCCGRR32 pKa = 11.84LDD34 pKa = 4.04CNNNSRR40 pKa = 11.84TTISQDD46 pKa = 3.28QVKK49 pKa = 10.04LAIRR53 pKa = 11.84TPATRR58 pKa = 11.84FLTEE62 pKa = 3.69NGGQYY67 pKa = 10.68VNAAIQLALDD77 pKa = 3.53EE78 pKa = 4.61AEE80 pKa = 4.1YY81 pKa = 10.98RR82 pKa = 11.84LGVTEE87 pKa = 3.82YY88 pKa = 11.28AQLFKK93 pKa = 11.31YY94 pKa = 10.32NKK96 pKa = 9.45APMGMRR102 pKa = 11.84SPEE105 pKa = 4.11EE106 pKa = 3.74TPEE109 pKa = 4.27FYY111 pKa = 10.39TFSEE115 pKa = 4.41PDD117 pKa = 2.96FDD119 pKa = 5.29AGG121 pKa = 4.14

Molecular weight: 13.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2447

121

1696

489.4

54.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.457 ± 1.089

2.043 ± 0.262

5.966 ± 0.801

7.07 ± 0.153

5.272 ± 0.158

6.702 ± 0.196

2.002 ± 0.543

4.904 ± 0.394

7.765 ± 0.714

10.421 ± 1.164

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.106 ± 0.308

4.046 ± 0.51

3.515 ± 0.392

2.615 ± 0.458

4.618 ± 0.621

7.56 ± 0.31

4.822 ± 0.551

6.947 ± 1.257

0.654 ± 0.183

3.515 ± 0.546
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski