Amino acid dipepetide frequency for Giardia muris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.088AlaAla: 8.088 ± 0.105	1.869AlaCys: 1.869 ± 0.048	4.046AlaAsp: 4.046 ± 0.047	5.401AlaGlu: 5.401 ± 0.084	2.989AlaPhe: 2.989 ± 0.036	4.704AlaGly: 4.704 ± 0.059	1.81AlaHis: 1.81 ± 0.028	4.655AlaIle: 4.655 ± 0.047	3.058AlaLys: 3.058 ± 0.047	11.241AlaLeu: 11.241 ± 0.125	1.635AlaMet: 1.635 ± 0.029	2.543AlaAsn: 2.543 ± 0.036	3.724AlaPro: 3.724 ± 0.049	3.547AlaGln: 3.547 ± 0.056	5.836AlaArg: 5.836 ± 0.061	6.57AlaSer: 6.57 ± 0.06	4.973AlaThr: 4.973 ± 0.056	4.889AlaVal: 4.889 ± 0.054	0.593AlaTrp: 0.593 ± 0.017	2.511AlaTyr: 2.511 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	1.628CysAla: 1.628 ± 0.036	0.441CysCys: 0.441 ± 0.015	1.06CysAsp: 1.06 ± 0.031	1.011CysGlu: 1.011 ± 0.026	0.749CysPhe: 0.749 ± 0.017	1.285CysGly: 1.285 ± 0.03	0.414CysHis: 0.414 ± 0.011	1.325CysIle: 1.325 ± 0.03	0.871CysLys: 0.871 ± 0.03	2.204CysLeu: 2.204 ± 0.035	0.396CysMet: 0.396 ± 0.013	0.714CysAsn: 0.714 ± 0.023	1.008CysPro: 1.008 ± 0.027	0.67CysGln: 0.67 ± 0.018	1.152CysArg: 1.152 ± 0.022	1.604CysSer: 1.604 ± 0.039	1.692CysThr: 1.692 ± 0.057	1.581CysVal: 1.581 ± 0.036	0.147CysTrp: 0.147 ± 0.007	0.69CysTyr: 0.69 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	4.754AspAla: 4.754 ± 0.055	1.006AspCys: 1.006 ± 0.025	3.251AspAsp: 3.251 ± 0.045	4.086AspGlu: 4.086 ± 0.045	1.99AspPhe: 1.99 ± 0.028	3.724AspGly: 3.724 ± 0.048	0.964AspHis: 0.964 ± 0.018	3.057AspIle: 3.057 ± 0.04	1.862AspLys: 1.862 ± 0.033	6.12AspLeu: 6.12 ± 0.06	1.095AspMet: 1.095 ± 0.019	1.598AspAsn: 1.598 ± 0.031	2.878AspPro: 2.878 ± 0.037	1.775AspGln: 1.775 ± 0.029	2.874AspArg: 2.874 ± 0.037	4.102AspSer: 4.102 ± 0.044	3.1AspThr: 3.1 ± 0.039	3.852AspVal: 3.852 ± 0.037	0.44AspTrp: 0.44 ± 0.013	1.518AspTyr: 1.518 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	6.718GluAla: 6.718 ± 0.081	1.299GluCys: 1.299 ± 0.033	3.305GluAsp: 3.305 ± 0.036	5.149GluGlu: 5.149 ± 0.068	1.689GluPhe: 1.689 ± 0.026	3.833GluGly: 3.833 ± 0.05	1.544GluHis: 1.544 ± 0.023	3.012GluIle: 3.012 ± 0.039	3.311GluLys: 3.311 ± 0.046	7.656GluLeu: 7.656 ± 0.086	1.504GluMet: 1.504 ± 0.025	2.263GluAsn: 2.263 ± 0.032	2.564GluPro: 2.564 ± 0.048	2.882GluGln: 2.882 ± 0.043	5.128GluArg: 5.128 ± 0.06	3.918GluSer: 3.918 ± 0.048	3.8GluThr: 3.8 ± 0.038	4.074GluVal: 4.074 ± 0.043	0.42GluTrp: 0.42 ± 0.013	2.071GluTyr: 2.071 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	2.383PheAla: 2.383 ± 0.034	0.734PheCys: 0.734 ± 0.018	1.996PheAsp: 1.996 ± 0.032	2.064PheGlu: 2.064 ± 0.029	1.375PhePhe: 1.375 ± 0.027	2.185PheGly: 2.185 ± 0.034	0.75PheHis: 0.75 ± 0.018	1.854PheIle: 1.854 ± 0.036	1.161PheLys: 1.161 ± 0.026	3.963PheLeu: 3.963 ± 0.057	0.617PheMet: 0.617 ± 0.017	1.2PheAsn: 1.2 ± 0.026	1.456PhePro: 1.456 ± 0.027	1.308PheGln: 1.308 ± 0.028	1.968PheArg: 1.968 ± 0.031	2.876PheSer: 2.876 ± 0.041	2.14PheThr: 2.14 ± 0.037	2.233PheVal: 2.233 ± 0.037	0.252PheTrp: 0.252 ± 0.011	1.161PheTyr: 1.161 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	4.158GlyAla: 4.158 ± 0.056	1.33GlyCys: 1.33 ± 0.038	3.077GlyAsp: 3.077 ± 0.035	3.422GlyGlu: 3.422 ± 0.047	2.093GlyPhe: 2.093 ± 0.037	3.74GlyGly: 3.74 ± 0.058	1.76GlyHis: 1.76 ± 0.04	3.003GlyIle: 3.003 ± 0.039	2.703GlyLys: 2.703 ± 0.047	6.249GlyLeu: 6.249 ± 0.049	1.562GlyMet: 1.562 ± 0.03	1.947GlyAsn: 1.947 ± 0.032	2.396GlyPro: 2.396 ± 0.039	2.038GlyGln: 2.038 ± 0.031	4.143GlyArg: 4.143 ± 0.054	4.655GlySer: 4.655 ± 0.053	4.217GlyThr: 4.217 ± 0.049	3.727GlyVal: 3.727 ± 0.041	0.698GlyTrp: 0.698 ± 0.021	2.047GlyTyr: 2.047 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.108HisAla: 2.108 ± 0.029	0.49HisCys: 0.49 ± 0.016	1.222HisAsp: 1.222 ± 0.019	1.504HisGlu: 1.504 ± 0.028	0.82HisPhe: 0.82 ± 0.018	1.448HisGly: 1.448 ± 0.028	0.534HisHis: 0.534 ± 0.016	1.336HisIle: 1.336 ± 0.025	0.758HisLys: 0.758 ± 0.019	2.775HisLeu: 2.775 ± 0.039	0.441HisMet: 0.441 ± 0.013	0.718HisAsn: 0.718 ± 0.014	1.41HisPro: 1.41 ± 0.023	0.86HisGln: 0.86 ± 0.02	1.525HisArg: 1.525 ± 0.026	1.723HisSer: 1.723 ± 0.029	1.506HisThr: 1.506 ± 0.023	1.53HisVal: 1.53 ± 0.026	0.177HisTrp: 0.177 ± 0.009	0.717HisTyr: 0.717 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	4.091IleAla: 4.091 ± 0.046	1.211IleCys: 1.211 ± 0.024	3.172IleAsp: 3.172 ± 0.04	3.152IleGlu: 3.152 ± 0.037	1.807IlePhe: 1.807 ± 0.035	2.805IleGly: 2.805 ± 0.037	1.253IleHis: 1.253 ± 0.025	2.613IleIle: 2.613 ± 0.044	1.814IleLys: 1.814 ± 0.027	5.442IleLeu: 5.442 ± 0.067	0.87IleMet: 0.87 ± 0.021	1.686IleAsn: 1.686 ± 0.028	3.18IlePro: 3.18 ± 0.043	2.179IleGln: 2.179 ± 0.034	3.457IleArg: 3.457 ± 0.043	4.221IleSer: 4.221 ± 0.047	3.127IleThr: 3.127 ± 0.042	3.276IleVal: 3.276 ± 0.047	0.402IleTrp: 0.402 ± 0.012	1.566IleTyr: 1.566 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.525LysAla: 3.525 ± 0.042	0.771LysCys: 0.771 ± 0.031	2.558LysAsp: 2.558 ± 0.034	3.277LysGlu: 3.277 ± 0.047	0.86LysPhe: 0.86 ± 0.022	2.409LysGly: 2.409 ± 0.035	0.97LysHis: 0.97 ± 0.019	1.644LysIle: 1.644 ± 0.028	2.188LysLys: 2.188 ± 0.036	3.852LysLeu: 3.852 ± 0.047	0.884LysMet: 0.884 ± 0.018	1.491LysAsn: 1.491 ± 0.03	1.82LysPro: 1.82 ± 0.032	1.753LysGln: 1.753 ± 0.03	2.839LysArg: 2.839 ± 0.041	2.38LysSer: 2.38 ± 0.035	2.649LysThr: 2.649 ± 0.035	2.19LysVal: 2.19 ± 0.032	0.22LysTrp: 0.22 ± 0.01	1.338LysTyr: 1.338 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	9.361LeuAla: 9.361 ± 0.089	2.228LeuCys: 2.228 ± 0.032	5.884LeuAsp: 5.884 ± 0.05	7.748LeuGlu: 7.748 ± 0.069	3.736LeuPhe: 3.736 ± 0.057	5.868LeuGly: 5.868 ± 0.05	2.819LeuHis: 2.819 ± 0.038	5.308LeuIle: 5.308 ± 0.061	4.562LeuLys: 4.562 ± 0.052	13.442LeuLeu: 13.442 ± 0.135	3.278LeuMet: 3.278 ± 0.069	3.418LeuAsn: 3.418 ± 0.04	5.633LeuPro: 5.633 ± 0.054	5.455LeuGln: 5.455 ± 0.057	8.159LeuArg: 8.159 ± 0.085	9.145LeuSer: 9.145 ± 0.074	6.789LeuThr: 6.789 ± 0.054	6.619LeuVal: 6.619 ± 0.057	0.736LeuTrp: 0.736 ± 0.019	2.991LeuTyr: 2.991 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	1.844MetAla: 1.844 ± 0.027	0.391MetCys: 0.391 ± 0.011	1.098MetAsp: 1.098 ± 0.02	1.307MetGlu: 1.307 ± 0.02	0.619MetPhe: 0.619 ± 0.017	1.163MetGly: 1.163 ± 0.025	0.69MetHis: 0.69 ± 0.015	0.989MetIle: 0.989 ± 0.02	0.92MetLys: 0.92 ± 0.019	2.372MetLeu: 2.372 ± 0.035	0.468MetMet: 0.468 ± 0.014	0.79MetAsn: 0.79 ± 0.019	0.965MetPro: 0.965 ± 0.02	1.027MetGln: 1.027 ± 0.023	1.517MetArg: 1.517 ± 0.026	1.677MetSer: 1.677 ± 0.022	1.528MetThr: 1.528 ± 0.023	1.155MetVal: 1.155 ± 0.022	0.176MetTrp: 0.176 ± 0.009	0.759MetTyr: 0.759 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	2.754AsnAla: 2.754 ± 0.043	0.783AsnCys: 0.783 ± 0.029	1.811AsnAsp: 1.811 ± 0.026	2.071AsnGlu: 2.071 ± 0.037	1.015AsnPhe: 1.015 ± 0.02	2.417AsnGly: 2.417 ± 0.044	0.688AsnHis: 0.688 ± 0.019	1.81AsnIle: 1.81 ± 0.031	1.232AsnLys: 1.232 ± 0.023	3.124AsnLeu: 3.124 ± 0.038	0.616AsnMet: 0.616 ± 0.013	1.094AsnAsn: 1.094 ± 0.023	1.786AsnPro: 1.786 ± 0.032	1.156AsnGln: 1.156 ± 0.024	1.876AsnArg: 1.876 ± 0.029	2.386AsnSer: 2.386 ± 0.04	2.161AsnThr: 2.161 ± 0.033	2.174AsnVal: 2.174 ± 0.034	0.24AsnTrp: 0.24 ± 0.008	1.013AsnTyr: 1.013 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	3.534ProAla: 3.534 ± 0.052	0.78ProCys: 0.78 ± 0.02	2.901ProAsp: 2.901 ± 0.034	3.608ProGlu: 3.608 ± 0.04	1.814ProPhe: 1.814 ± 0.033	2.906ProGly: 2.906 ± 0.045	1.197ProHis: 1.197 ± 0.023	2.651ProIle: 2.651 ± 0.035	1.835ProLys: 1.835 ± 0.043	5.179ProLeu: 5.179 ± 0.056	0.955ProMet: 0.955 ± 0.021	1.675ProAsn: 1.675 ± 0.03	3.617ProPro: 3.617 ± 0.061	1.935ProGln: 1.935 ± 0.039	2.858ProArg: 2.858 ± 0.046	4.64ProSer: 4.64 ± 0.054	3.642ProThr: 3.642 ± 0.046	2.921ProVal: 2.921 ± 0.041	0.336ProTrp: 0.336 ± 0.011	1.591ProTyr: 1.591 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.732GlnAla: 3.732 ± 0.053	0.646GlnCys: 0.646 ± 0.016	2.179GlnAsp: 2.179 ± 0.031	2.86GlnGlu: 2.86 ± 0.045	1.095GlnPhe: 1.095 ± 0.023	2.303GlnGly: 2.303 ± 0.03	0.973GlnHis: 0.973 ± 0.02	1.792GlnIle: 1.792 ± 0.03	2.017GlnLys: 2.017 ± 0.037	4.723GlnLeu: 4.723 ± 0.067	0.956GlnMet: 0.956 ± 0.018	1.497GlnAsn: 1.497 ± 0.024	1.881GlnPro: 1.881 ± 0.037	2.025GlnGln: 2.025 ± 0.04	3.022GlnArg: 3.022 ± 0.042	2.648GlnSer: 2.648 ± 0.042	2.78GlnThr: 2.78 ± 0.038	2.27GlnVal: 2.27 ± 0.032	0.277GlnTrp: 0.277 ± 0.011	1.262GlnTyr: 1.262 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	5.581ArgAla: 5.581 ± 0.067	1.272ArgCys: 1.272 ± 0.023	3.607ArgAsp: 3.607 ± 0.047	4.665ArgGlu: 4.665 ± 0.06	2.215ArgPhe: 2.215 ± 0.039	3.748ArgGly: 3.748 ± 0.049	1.608ArgHis: 1.608 ± 0.028	3.475ArgIle: 3.475 ± 0.041	2.732ArgLys: 2.732 ± 0.038	7.915ArgLeu: 7.915 ± 0.069	1.44ArgMet: 1.44 ± 0.025	2.119ArgAsn: 2.119 ± 0.027	3.258ArgPro: 3.258 ± 0.05	2.901ArgGln: 2.901 ± 0.041	5.326ArgArg: 5.326 ± 0.066	4.698ArgSer: 4.698 ± 0.05	4.286ArgThr: 4.286 ± 0.038	4.031ArgVal: 4.031 ± 0.045	0.44ArgTrp: 0.44 ± 0.014	2.086ArgTyr: 2.086 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	5.806SerAla: 5.806 ± 0.051	1.542SerCys: 1.542 ± 0.04	3.942SerAsp: 3.942 ± 0.04	4.503SerGlu: 4.503 ± 0.046	3.027SerPhe: 3.027 ± 0.043	4.789SerGly: 4.789 ± 0.053	1.698SerHis: 1.698 ± 0.027	4.281SerIle: 4.281 ± 0.048	2.848SerLys: 2.848 ± 0.041	9.053SerLeu: 9.053 ± 0.082	1.613SerMet: 1.613 ± 0.026	2.494SerAsn: 2.494 ± 0.037	4.187SerPro: 4.187 ± 0.053	3.083SerGln: 3.083 ± 0.043	4.884SerArg: 4.884 ± 0.052	7.46SerSer: 7.46 ± 0.085	5.374SerThr: 5.374 ± 0.051	4.629SerVal: 4.629 ± 0.046	0.531SerTrp: 0.531 ± 0.014	2.1SerTyr: 2.1 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	6.363ThrAla: 6.363 ± 0.078	1.52ThrCys: 1.52 ± 0.053	3.344ThrAsp: 3.344 ± 0.034	3.875ThrGlu: 3.875 ± 0.038	2.163ThrPhe: 2.163 ± 0.037	3.905ThrGly: 3.905 ± 0.042	1.477ThrHis: 1.477 ± 0.023	3.455ThrIle: 3.455 ± 0.042	2.313ThrLys: 2.313 ± 0.037	7.054ThrLeu: 7.054 ± 0.065	1.145ThrMet: 1.145 ± 0.022	2.02ThrAsn: 2.02 ± 0.036	3.87ThrPro: 3.87 ± 0.042	2.434ThrGln: 2.434 ± 0.035	4.018ThrArg: 4.018 ± 0.043	5.308ThrSer: 5.308 ± 0.061	4.638ThrThr: 4.638 ± 0.055	3.77ThrVal: 3.77 ± 0.044	0.403ThrTrp: 0.403 ± 0.014	1.718ThrTyr: 1.718 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	4.899ValAla: 4.899 ± 0.052	1.513ValCys: 1.513 ± 0.034	3.538ValAsp: 3.538 ± 0.04	4.128ValGlu: 4.128 ± 0.043	2.197ValPhe: 2.197 ± 0.035	3.454ValGly: 3.454 ± 0.038	1.517ValHis: 1.517 ± 0.027	3.225ValIle: 3.225 ± 0.04	2.076ValLys: 2.076 ± 0.03	6.627ValLeu: 6.627 ± 0.059	1.195ValMet: 1.195 ± 0.023	1.547ValAsn: 1.547 ± 0.026	3.246ValPro: 3.246 ± 0.043	2.512ValGln: 2.512 ± 0.037	4.356ValArg: 4.356 ± 0.044	4.94ValSer: 4.94 ± 0.045	3.784ValThr: 3.784 ± 0.045	3.992ValVal: 3.992 ± 0.049	0.549ValTrp: 0.549 ± 0.017	1.894ValTyr: 1.894 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	0.615TrpAla: 0.615 ± 0.017	0.161TrpCys: 0.161 ± 0.007	0.389TrpAsp: 0.389 ± 0.012	0.372TrpGlu: 0.372 ± 0.012	0.337TrpPhe: 0.337 ± 0.012	0.37TrpGly: 0.37 ± 0.013	0.222TrpHis: 0.222 ± 0.01	0.349TrpIle: 0.349 ± 0.011	0.325TrpLys: 0.325 ± 0.011	0.742TrpLeu: 0.742 ± 0.017	0.162TrpMet: 0.162 ± 0.008	0.282TrpAsn: 0.282 ± 0.01	0.318TrpPro: 0.318 ± 0.011	0.218TrpGln: 0.218 ± 0.011	0.533TrpArg: 0.533 ± 0.015	0.555TrpSer: 0.555 ± 0.014	0.66TrpThr: 0.66 ± 0.02	0.386TrpVal: 0.386 ± 0.014	0.083TrpTrp: 0.083 ± 0.006	0.283TrpTyr: 0.283 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.742TyrAla: 2.742 ± 0.04	0.655TyrCys: 0.655 ± 0.017	1.668TyrAsp: 1.668 ± 0.027	1.935TyrGlu: 1.935 ± 0.025	1.086TyrPhe: 1.086 ± 0.024	1.907TyrGly: 1.907 ± 0.032	0.713TyrHis: 0.713 ± 0.018	1.494TyrIle: 1.494 ± 0.028	1.034TyrLys: 1.034 ± 0.019	3.444TyrLeu: 3.444 ± 0.048	0.621TyrMet: 0.621 ± 0.014	1.111TyrAsn: 1.111 ± 0.026	1.379TyrPro: 1.379 ± 0.024	1.182TyrGln: 1.182 ± 0.024	1.929TyrArg: 1.929 ± 0.023	2.338TyrSer: 2.338 ± 0.036	1.948TyrThr: 1.948 ± 0.027	1.89TyrVal: 1.89 ± 0.029	0.242TyrTrp: 0.242 ± 0.011	1.042TyrTyr: 1.042 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4560 proteins (2636066 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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