Bovine nidovirus TCH5

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Tornidovirineae; Tobaniviridae; Remotovirinae; Bostovirus; Bosnitovirus; Bovine nidovirus 1

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6PN25|A0A0F6PN25_9NIDO Non-structural protein 6 OS=Bovine nidovirus TCH5 OX=1631554 PE=4 SV=1
MM1 pKa = 7.68ANRR4 pKa = 11.84RR5 pKa = 11.84SFSLFCMCIAFIMVLSEE22 pKa = 4.67KK23 pKa = 10.53DD24 pKa = 3.37ALCISIACTPKK35 pKa = 9.92FVNGVLYY42 pKa = 9.04EE43 pKa = 4.19CCEE46 pKa = 4.16EE47 pKa = 4.69YY48 pKa = 11.27DD49 pKa = 3.47MCKK52 pKa = 10.52NPVTAGISVSSPNPLEE68 pKa = 4.63HH69 pKa = 7.76DD70 pKa = 3.87SVTSVTPSLGMVSPGAKK87 pKa = 9.66KK88 pKa = 10.38

Molecular weight: 9.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6PMZ2|A0A0F6PMZ2_9NIDO Non-structural protein 6 OS=Bovine nidovirus TCH5 OX=1631554 PE=4 SV=1
MM1 pKa = 8.57IFMCTNKK8 pKa = 10.11RR9 pKa = 11.84VGKK12 pKa = 9.29MSNGRR17 pKa = 11.84GNFRR21 pKa = 11.84RR22 pKa = 11.84SQSVPKK28 pKa = 10.15QFKK31 pKa = 10.48PNMQRR36 pKa = 11.84QRR38 pKa = 11.84SRR40 pKa = 11.84SRR42 pKa = 11.84EE43 pKa = 3.49RR44 pKa = 11.84RR45 pKa = 11.84VRR47 pKa = 11.84FDD49 pKa = 3.38RR50 pKa = 11.84NTRR53 pKa = 11.84FNDD56 pKa = 2.9NGRR59 pKa = 11.84QYY61 pKa = 10.99RR62 pKa = 11.84KK63 pKa = 7.4EE64 pKa = 3.67QRR66 pKa = 11.84FGRR69 pKa = 11.84NSRR72 pKa = 11.84YY73 pKa = 7.31QRR75 pKa = 11.84RR76 pKa = 11.84QQQQYY81 pKa = 7.27QQSRR85 pKa = 11.84RR86 pKa = 11.84NGPSTGVGRR95 pKa = 11.84KK96 pKa = 8.87LVLRR100 pKa = 11.84TMVQQNRR107 pKa = 11.84YY108 pKa = 9.14HH109 pKa = 6.92YY110 pKa = 8.35YY111 pKa = 9.42TEE113 pKa = 4.29LAAGDD118 pKa = 3.84VRR120 pKa = 11.84NEE122 pKa = 3.69ADD124 pKa = 3.48RR125 pKa = 11.84RR126 pKa = 11.84ALFVLAQEE134 pKa = 4.58IKK136 pKa = 10.35NAVIQGAGTLSASEE150 pKa = 4.32DD151 pKa = 3.71TVDD154 pKa = 3.63YY155 pKa = 10.62HH156 pKa = 7.16VQFQPRR162 pKa = 11.84SVILSGTFSTGGQSEE177 pKa = 4.31AA178 pKa = 4.13

Molecular weight: 20.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

9738

88

5110

1391.1

157.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.079 ± 0.488

3.266 ± 0.145

4.96 ± 0.204

6.131 ± 0.501

6.059 ± 0.155

6.346 ± 0.157

1.581 ± 0.082

4.539 ± 0.316

7.209 ± 0.45

8.256 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.185

4.868 ± 0.248

3.45 ± 0.099

3.871 ± 0.301

3.861 ± 0.547

6.387 ± 0.259

3.327 ± 0.128

10.557 ± 0.375

1.201 ± 0.086

5.36 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski