Only Syngen Nebraska virus 5

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Chlorovirus; unclassified Chlorovirus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 355 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0F9M0|A0A1J0F9M0_9PHYC Uncharacterized protein OS=Only Syngen Nebraska virus 5 OX=1917232 GN=OS5_176L PE=4 SV=1
MM1 pKa = 7.68GLPPLEE7 pKa = 4.69LVLNDD12 pKa = 3.98DD13 pKa = 3.65EE14 pKa = 4.99RR15 pKa = 11.84ALGVLYY21 pKa = 10.59EE22 pKa = 4.31GALTGEE28 pKa = 4.73ALLALPGVLYY38 pKa = 10.85DD39 pKa = 4.94GVLPLPGVLTGEE51 pKa = 4.68GEE53 pKa = 4.43LDD55 pKa = 3.58RR56 pKa = 11.84PLAPLLLLPDD66 pKa = 4.87PFQEE70 pKa = 4.63TILLDD75 pKa = 3.54IYY77 pKa = 10.09YY78 pKa = 10.07IKK80 pKa = 10.5II81 pKa = 3.56

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0FA23|A0A1J0FA23_9PHYC Uncharacterized protein OS=Only Syngen Nebraska virus 5 OX=1917232 GN=OS5_322L PE=4 SV=1
MM1 pKa = 7.38DD2 pKa = 3.84TKK4 pKa = 10.88LRR6 pKa = 11.84RR7 pKa = 11.84QTLKK11 pKa = 10.09RR12 pKa = 11.84DD13 pKa = 3.29EE14 pKa = 4.81RR15 pKa = 11.84KK16 pKa = 10.06LSTRR20 pKa = 11.84EE21 pKa = 3.71RR22 pKa = 11.84QEE24 pKa = 4.32LEE26 pKa = 3.86RR27 pKa = 11.84QRR29 pKa = 11.84LTRR32 pKa = 11.84MRR34 pKa = 11.84RR35 pKa = 11.84DD36 pKa = 3.75AKK38 pKa = 10.08PMPMIINSKK47 pKa = 6.99TPRR50 pKa = 11.84TVAPVRR56 pKa = 11.84PRR58 pKa = 11.84SRR60 pKa = 11.84SPPSLFKK67 pKa = 10.72RR68 pKa = 11.84IGSGGIKK75 pKa = 10.16KK76 pKa = 9.54IRR78 pKa = 11.84QQTIKK83 pKa = 10.61NEE85 pKa = 4.02RR86 pKa = 11.84GRR88 pKa = 11.84TYY90 pKa = 10.88AVKK93 pKa = 10.4KK94 pKa = 8.35ITGPRR99 pKa = 11.84VSCSKK104 pKa = 10.9VLTFAQQGAICWFTALITTLFFSQYY129 pKa = 8.25TRR131 pKa = 11.84VVMKK135 pKa = 9.44IHH137 pKa = 6.37ARR139 pKa = 11.84QMVKK143 pKa = 10.53YY144 pKa = 10.33SNTKK148 pKa = 10.05QIAEE152 pKa = 5.05AILEE156 pKa = 4.55LIKK159 pKa = 10.93GYY161 pKa = 10.93DD162 pKa = 3.15SGKK165 pKa = 8.2VSRR168 pKa = 11.84RR169 pKa = 11.84VVEE172 pKa = 4.33SMQPHH177 pKa = 5.91QFLKK181 pKa = 10.64DD182 pKa = 3.4LRR184 pKa = 11.84ATRR187 pKa = 11.84SNLFDD192 pKa = 3.3STLNGSSEE200 pKa = 3.95AHH202 pKa = 5.72YY203 pKa = 10.83NPYY206 pKa = 8.62VHH208 pKa = 7.36KK209 pKa = 10.41ILGFLRR215 pKa = 11.84VPHH218 pKa = 6.88LGLGIVGGKK227 pKa = 9.03IVYY230 pKa = 10.41SGFNVDD236 pKa = 5.49LPLDD240 pKa = 4.05SKK242 pKa = 11.32LWAQAMKK249 pKa = 8.77TQSPRR254 pKa = 11.84GTFVDD259 pKa = 3.83TNNPEE264 pKa = 3.81VLLLHH269 pKa = 7.03RR270 pKa = 11.84DD271 pKa = 3.3GGEE274 pKa = 4.01EE275 pKa = 4.06YY276 pKa = 11.16VQGLWGRR283 pKa = 11.84PRR285 pKa = 11.84PAIGSVAGISSDD297 pKa = 2.81IHH299 pKa = 6.79NKK301 pKa = 8.97FIKK304 pKa = 10.59YY305 pKa = 9.9NGRR308 pKa = 11.84NYY310 pKa = 10.24ILDD313 pKa = 3.58SCILGGEE320 pKa = 4.26VRR322 pKa = 11.84TPSCSVAHH330 pKa = 7.16AIAGVTCNNKK340 pKa = 9.49RR341 pKa = 11.84FIYY344 pKa = 10.29NGWTARR350 pKa = 11.84SADD353 pKa = 3.61PAMSGSGSVIRR364 pKa = 11.84DD365 pKa = 3.77MPCSLMPADD374 pKa = 3.42WASNKK379 pKa = 9.67SFCINTSACKK389 pKa = 10.15MNIAKK394 pKa = 9.89PNQLSKK400 pKa = 10.41EE401 pKa = 4.06LCFNGVARR409 pKa = 11.84STVVYY414 pKa = 10.23IRR416 pKa = 11.84SDD418 pKa = 3.43LAKK421 pKa = 10.41LGGYY425 pKa = 9.25KK426 pKa = 10.46VIGKK430 pKa = 9.17QIQRR434 pKa = 11.84VRR436 pKa = 11.84KK437 pKa = 8.83RR438 pKa = 3.17

Molecular weight: 49.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

355

0

355

98420

42

1506

277.2

31.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.417 ± 0.153

1.43 ± 0.09

5.712 ± 0.182

5.439 ± 0.233

4.583 ± 0.1

6.514 ± 0.26

1.736 ± 0.087

6.973 ± 0.128

7.901 ± 0.332

7.064 ± 0.119

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.078

6.014 ± 0.258

5.141 ± 0.237

2.878 ± 0.097

4.201 ± 0.129

6.842 ± 0.147

6.527 ± 0.188

7.101 ± 0.149

1.071 ± 0.046

3.859 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski