Enterobacteria phage KhF2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Felixounavirus; unclassified Felixounavirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384SJ87|A0A384SJ87_9CAUD Exonuclease OS=Enterobacteria phage KhF2 OX=1651204 PE=4 SV=1
MM1 pKa = 7.6IKK3 pKa = 10.48LNQKK7 pKa = 7.22QLEE10 pKa = 4.47WIKK13 pKa = 11.01DD14 pKa = 3.66YY15 pKa = 11.37ASEE18 pKa = 4.98CGSCEE23 pKa = 4.02KK24 pKa = 10.95NHH26 pKa = 5.38VKK28 pKa = 10.75YY29 pKa = 9.81STFHH33 pKa = 5.8TAFTLYY39 pKa = 10.28ISDD42 pKa = 3.94NVLSDD47 pKa = 3.67SVEE50 pKa = 4.49DD51 pKa = 5.02GVALPNEE58 pKa = 4.63LLDD61 pKa = 3.82KK62 pKa = 10.74LAVVTGTWSEE72 pKa = 3.99EE73 pKa = 4.08DD74 pKa = 3.35GHH76 pKa = 6.2EE77 pKa = 4.44LSDD80 pKa = 4.11DD81 pKa = 3.74VVFYY85 pKa = 11.11SLEE88 pKa = 4.18NIMNPEE94 pKa = 4.39YY95 pKa = 10.77IMLMTCAQDD104 pKa = 4.41CVPLQNFIKK113 pKa = 9.16EE114 pKa = 4.04HH115 pKa = 6.53CEE117 pKa = 3.65EE118 pKa = 5.33FITKK122 pKa = 9.05QVPCQVVFEE131 pKa = 4.39

Molecular weight: 15.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384TAX2|A0A384TAX2_9CAUD Uncharacterized protein OS=Enterobacteria phage KhF2 OX=1651204 PE=4 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84VILARR7 pKa = 11.84DD8 pKa = 3.05KK9 pKa = 10.42KK10 pKa = 8.04TRR12 pKa = 11.84KK13 pKa = 9.42LIRR16 pKa = 11.84SAVFIGRR23 pKa = 11.84NEE25 pKa = 4.32VIPFTANDD33 pKa = 3.37VIAYY37 pKa = 7.86RR38 pKa = 11.84KK39 pKa = 9.83NLVFSDD45 pKa = 4.02CLRR48 pKa = 11.84GFISQPLEE56 pKa = 4.08DD57 pKa = 4.1FKK59 pKa = 11.65SRR61 pKa = 11.84CGKK64 pKa = 10.67GIIIQEE70 pKa = 4.21AIINGG75 pKa = 3.71

Molecular weight: 8.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

20604

48

906

236.8

26.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.217 ± 0.301

1.233 ± 0.128

6.242 ± 0.198

6.897 ± 0.328

4.189 ± 0.182

6.504 ± 0.229

1.951 ± 0.156

5.965 ± 0.174

7.79 ± 0.335

7.984 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.796 ± 0.162

5.348 ± 0.247

3.053 ± 0.157

3.543 ± 0.211

4.116 ± 0.167

6.465 ± 0.216

6.125 ± 0.348

7.052 ± 0.201

1.218 ± 0.107

4.315 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski