Aeromonas phage MJG

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Uliginvirus; unclassified Uliginvirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J5ZZH3|A0A5J5ZZH3_9CAUD Putative tail tubular protein A OS=Aeromonas phage MJG OX=2510451 PE=4 SV=1
MM1 pKa = 7.25EE2 pKa = 4.01QTEE5 pKa = 4.12EE6 pKa = 4.36LEE8 pKa = 4.07LFEE11 pKa = 6.13KK12 pKa = 10.43IFSSIEE18 pKa = 3.58QGLPIPAQLISKK30 pKa = 10.24AEE32 pKa = 3.82EE33 pKa = 3.39HH34 pKa = 6.94GIRR37 pKa = 11.84VDD39 pKa = 4.02AIKK42 pKa = 10.72DD43 pKa = 3.31IVASLGTEE51 pKa = 3.78EE52 pKa = 5.87GEE54 pKa = 4.96DD55 pKa = 4.0DD56 pKa = 4.22FDD58 pKa = 4.76EE59 pKa = 5.83KK60 pKa = 11.14FDD62 pKa = 3.68

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J5ZZG1|A0A5J5ZZG1_9CAUD Uncharacterized protein OS=Aeromonas phage MJG OX=2510451 PE=4 SV=1
MM1 pKa = 7.23TSANVVVDD9 pKa = 4.53HH10 pKa = 5.54NHH12 pKa = 4.92KK13 pKa = 9.61TGVIRR18 pKa = 11.84AALPRR23 pKa = 11.84SINGLEE29 pKa = 4.14GKK31 pKa = 10.0IKK33 pKa = 10.74GLLTRR38 pKa = 11.84WGSCTNEE45 pKa = 3.52VEE47 pKa = 4.3MIKK50 pKa = 9.52MLEE53 pKa = 4.33AIAEE57 pKa = 4.21YY58 pKa = 9.55WRR60 pKa = 11.84LHH62 pKa = 6.0RR63 pKa = 11.84VPQTEE68 pKa = 4.66WIHH71 pKa = 5.42PTHH74 pKa = 6.84LTPQEE79 pKa = 3.9ARR81 pKa = 11.84EE82 pKa = 4.0KK83 pKa = 10.93ANAKK87 pKa = 9.79ARR89 pKa = 11.84ARR91 pKa = 11.84YY92 pKa = 8.32AAAKK96 pKa = 9.6KK97 pKa = 10.3

Molecular weight: 10.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

12935

58

1259

369.6

41.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.014 ± 0.618

0.99 ± 0.151

6.386 ± 0.259

7.089 ± 0.323

3.943 ± 0.262

7.252 ± 0.367

2.265 ± 0.185

4.948 ± 0.212

6.208 ± 0.273

7.739 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.255 ± 0.221

4.623 ± 0.188

4.043 ± 0.205

4.02 ± 0.255

5.365 ± 0.248

5.589 ± 0.236

5.597 ± 0.208

6.765 ± 0.25

1.384 ± 0.183

3.525 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski