Mycobacterium phage Alsfro

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8FXD7|W8FXD7_9CAUD Uncharacterized protein OS=Mycobacterium phage Alsfro OX=1458724 GN=70 PE=4 SV=1
MM1 pKa = 7.76SDD3 pKa = 3.77FPSAEE8 pKa = 3.68QQARR12 pKa = 11.84NRR14 pKa = 11.84LINSIEE20 pKa = 3.91NTIWNALEE28 pKa = 4.13QQDD31 pKa = 3.91GPYY34 pKa = 10.15VDD36 pKa = 5.56RR37 pKa = 11.84EE38 pKa = 4.09MGMVDD43 pKa = 3.51ASGAGLDD50 pKa = 3.74MTAVAAAVADD60 pKa = 3.53IFVDD64 pKa = 4.98SEE66 pKa = 5.14DD67 pKa = 3.74DD68 pKa = 4.06CSWCHH73 pKa = 6.02SPCAAGGWSEE83 pKa = 3.97PADD86 pKa = 3.98VEE88 pKa = 4.6SLPALLCLPSEE99 pKa = 4.6VAEE102 pKa = 3.99QMVNEE107 pKa = 3.98WLLRR111 pKa = 3.92

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8FQ64|W8FQ64_9CAUD Uncharacterized protein OS=Mycobacterium phage Alsfro OX=1458724 GN=7 PE=4 SV=1
MM1 pKa = 7.35SWDD4 pKa = 3.33SSDD7 pKa = 3.36RR8 pKa = 11.84RR9 pKa = 11.84SRR11 pKa = 11.84LPADD15 pKa = 3.38WPRR18 pKa = 11.84IRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 3.77VLRR25 pKa = 11.84AAGHH29 pKa = 6.46RR30 pKa = 11.84CQIRR34 pKa = 11.84YY35 pKa = 9.29PDD37 pKa = 3.72ICTGMATEE45 pKa = 4.27VDD47 pKa = 3.34HH48 pKa = 7.06VRR50 pKa = 11.84YY51 pKa = 9.51RR52 pKa = 11.84DD53 pKa = 3.51EE54 pKa = 4.59SSPLQVSCRR63 pKa = 11.84PCHH66 pKa = 5.53ARR68 pKa = 11.84KK69 pKa = 9.57SAMEE73 pKa = 4.09GVAQRR78 pKa = 11.84AKK80 pKa = 10.29LRR82 pKa = 11.84AMKK85 pKa = 9.94KK86 pKa = 9.63RR87 pKa = 11.84PPPRR91 pKa = 11.84HH92 pKa = 5.63PGRR95 pKa = 11.84RR96 pKa = 11.84NKK98 pKa = 10.77EE99 pKa = 3.81EE100 pKa = 3.84TT101 pKa = 3.39

Molecular weight: 11.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

15922

23

824

162.5

17.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.898 ± 0.385

0.647 ± 0.102

6.544 ± 0.202

6.739 ± 0.264

3.222 ± 0.156

8.579 ± 0.496

1.884 ± 0.144

4.937 ± 0.234

4.252 ± 0.244

8.535 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.161 ± 0.143

3.165 ± 0.145

5.54 ± 0.237

3.191 ± 0.146

6.299 ± 0.428

5.948 ± 0.258

6.224 ± 0.287

7.311 ± 0.25

2.066 ± 0.128

2.858 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski