Wenzhou narna-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJ26|A0A1L3KJ26_9VIRU Uncharacterized protein OS=Wenzhou narna-like virus 4 OX=1923579 PE=4 SV=1
MM1 pKa = 7.76QEE3 pKa = 4.17EE4 pKa = 5.25LSPQQNALQFLNRR17 pKa = 11.84ISAQSTIGMWTDD29 pKa = 3.09STPPFPCPSGKK40 pKa = 9.94KK41 pKa = 8.79EE42 pKa = 3.68GRR44 pKa = 11.84LAFVANTKK52 pKa = 10.58SGFNWLVKK60 pKa = 10.31LPKK63 pKa = 10.18SLLEE67 pKa = 4.1SVALEE72 pKa = 3.99RR73 pKa = 11.84VTTKK77 pKa = 10.65LGEE80 pKa = 4.04VRR82 pKa = 11.84TWQPQASEE90 pKa = 4.51SEE92 pKa = 4.54DD93 pKa = 3.84GIKK96 pKa = 8.94PTGLNYY102 pKa = 10.75SKK104 pKa = 10.95VILVPTIGDD113 pKa = 3.83CEE115 pKa = 4.32SWRR118 pKa = 11.84AAADD122 pKa = 3.99FCCGYY127 pKa = 10.65LSVIEE132 pKa = 4.63DD133 pKa = 3.96SYY135 pKa = 12.22AKK137 pKa = 10.24ARR139 pKa = 11.84KK140 pKa = 9.3LDD142 pKa = 3.57FHH144 pKa = 6.56TATTPFRR151 pKa = 11.84HH152 pKa = 5.78LVKK155 pKa = 9.63IDD157 pKa = 4.06KK158 pKa = 10.1SQQTAWIKK166 pKa = 10.8YY167 pKa = 5.6YY168 pKa = 9.85TAAPMSQILGEE179 pKa = 4.39TLPDD183 pKa = 3.71TPDD186 pKa = 3.04SSRR189 pKa = 11.84TPVFFSGKK197 pKa = 8.19FRR199 pKa = 11.84KK200 pKa = 9.37FMHH203 pKa = 5.21QRR205 pKa = 11.84CLKK208 pKa = 8.54GTRR211 pKa = 11.84DD212 pKa = 3.36LRR214 pKa = 11.84NIRR217 pKa = 11.84NAFNLLQGVKK227 pKa = 9.84KK228 pKa = 10.48ACQEE232 pKa = 3.62ISKK235 pKa = 10.74DD236 pKa = 4.14FILEE240 pKa = 3.98AYY242 pKa = 8.46KK243 pKa = 10.45KK244 pKa = 9.62HH245 pKa = 6.48RR246 pKa = 11.84KK247 pKa = 7.23TMEE250 pKa = 4.22TPCAGPPATEE260 pKa = 4.08LHH262 pKa = 4.77QAKK265 pKa = 10.5YY266 pKa = 10.68KK267 pKa = 9.76MLWTQGRR274 pKa = 11.84WIDD277 pKa = 2.99RR278 pKa = 11.84WKK280 pKa = 10.48KK281 pKa = 9.05YY282 pKa = 10.67KK283 pKa = 10.04PVLFKK288 pKa = 10.97PPGPAATLEE297 pKa = 4.41CPRR300 pKa = 11.84SEE302 pKa = 4.53GGGSQYY308 pKa = 11.32AFEE311 pKa = 4.2QNRR314 pKa = 11.84VFDD317 pKa = 3.8ARR319 pKa = 11.84EE320 pKa = 3.62IAVQSAIVNEE330 pKa = 4.04QLWFFFDD337 pKa = 3.41RR338 pKa = 11.84LGIRR342 pKa = 11.84DD343 pKa = 4.44AFLKK347 pKa = 7.73TQKK350 pKa = 9.65IDD352 pKa = 3.47EE353 pKa = 4.47VSPFWTWPEE362 pKa = 3.87SEE364 pKa = 4.07EE365 pKa = 4.09TGRR368 pKa = 11.84VQTVPTRR375 pKa = 11.84CLSPEE380 pKa = 3.85GKK382 pKa = 9.45IFYY385 pKa = 9.24QWQHH389 pKa = 5.73YY390 pKa = 9.18SPTWEE395 pKa = 4.18EE396 pKa = 4.33VFALYY401 pKa = 10.7NEE403 pKa = 4.44EE404 pKa = 4.14DD405 pKa = 3.69PKK407 pKa = 11.38NRR409 pKa = 11.84TVMVEE414 pKa = 4.18GIIEE418 pKa = 4.07PLKK421 pKa = 10.91LRR423 pKa = 11.84TITKK427 pKa = 9.54GPCHH431 pKa = 6.68RR432 pKa = 11.84KK433 pKa = 8.64WLSQSLQRR441 pKa = 11.84EE442 pKa = 4.17MADD445 pKa = 3.59CLDD448 pKa = 4.28GFWQFKK454 pKa = 10.0LNKK457 pKa = 9.79ANTDD461 pKa = 2.79HH462 pKa = 7.7RR463 pKa = 11.84MVTRR467 pKa = 11.84LNAACVRR474 pKa = 11.84LHH476 pKa = 6.54SKK478 pKa = 10.2QDD480 pKa = 3.05SFTPDD485 pKa = 2.9TPMWWCSGDD494 pKa = 3.69YY495 pKa = 10.59KK496 pKa = 11.0SATDD500 pKa = 4.2SISIHH505 pKa = 5.17HH506 pKa = 6.59TKK508 pKa = 10.6AALEE512 pKa = 4.07TLLNCTSPEE521 pKa = 4.05RR522 pKa = 11.84VNEE525 pKa = 3.92ACKK528 pKa = 10.25RR529 pKa = 11.84LYY531 pKa = 10.3RR532 pKa = 11.84DD533 pKa = 3.34EE534 pKa = 5.64LYY536 pKa = 10.64EE537 pKa = 5.24QIVQYY542 pKa = 9.52PKK544 pKa = 8.46WTGIPDD550 pKa = 3.51VQQVNGQLMGSVLSFPILCVINFVAYY576 pKa = 7.3WQSLEE581 pKa = 3.87EE582 pKa = 4.81HH583 pKa = 6.48YY584 pKa = 11.34GCTLNVRR591 pKa = 11.84SVPCLIHH598 pKa = 7.67GDD600 pKa = 4.08DD601 pKa = 3.9ILFKK605 pKa = 8.91TTHH608 pKa = 4.69EE609 pKa = 4.78HH610 pKa = 5.12YY611 pKa = 10.74AVWEE615 pKa = 4.07EE616 pKa = 4.12TIKK619 pKa = 11.16LFGLQKK625 pKa = 10.69SVGKK629 pKa = 10.4NYY631 pKa = 8.62FHH633 pKa = 7.28PKK635 pKa = 9.73VFTIDD640 pKa = 3.21SEE642 pKa = 4.47LWVEE646 pKa = 4.54SKK648 pKa = 10.81RR649 pKa = 11.84SDD651 pKa = 3.08GFHH654 pKa = 6.19FKK656 pKa = 10.24KK657 pKa = 10.86YY658 pKa = 10.44SGINVGSLLKK668 pKa = 10.84SKK670 pKa = 10.95VDD672 pKa = 3.49GRR674 pKa = 11.84NDD676 pKa = 3.54YY677 pKa = 10.68QRR679 pKa = 11.84LPIWDD684 pKa = 4.34KK685 pKa = 10.64FNSSIRR691 pKa = 11.84GAQDD695 pKa = 2.67KK696 pKa = 10.35EE697 pKa = 4.39LYY699 pKa = 10.12LKK701 pKa = 10.78RR702 pKa = 11.84FLHH705 pKa = 4.97YY706 pKa = 9.96HH707 pKa = 6.47KK708 pKa = 10.49STIKK712 pKa = 10.56PMTWTKK718 pKa = 11.15AGVLNLFLPRR728 pKa = 11.84EE729 pKa = 3.86RR730 pKa = 11.84GGIGFEE736 pKa = 4.82LPWKK740 pKa = 9.79PNEE743 pKa = 4.11IPIKK747 pKa = 10.54EE748 pKa = 4.27NGTPLVAVTKK758 pKa = 10.13HH759 pKa = 4.84QLNLASALCNSFRR772 pKa = 11.84EE773 pKa = 4.29AGPLKK778 pKa = 10.5SLAIVAVVDD787 pKa = 3.52KK788 pKa = 11.07TDD790 pKa = 2.91ATTEE794 pKa = 3.54KK795 pKa = 10.35RR796 pKa = 11.84YY797 pKa = 9.88RR798 pKa = 11.84LPFQEE803 pKa = 4.23VWKK806 pKa = 10.79HH807 pKa = 4.22EE808 pKa = 4.07STYY811 pKa = 11.09ARR813 pKa = 11.84EE814 pKa = 4.35IPGEE818 pKa = 3.96LADD821 pKa = 5.36PILSEE826 pKa = 4.37VPSGKK831 pKa = 9.47PEE833 pKa = 3.85EE834 pKa = 4.13YY835 pKa = 10.09RR836 pKa = 11.84LRR838 pKa = 11.84KK839 pKa = 9.62PEE841 pKa = 3.68IRR843 pKa = 11.84SLARR847 pKa = 11.84GPKK850 pKa = 8.81RR851 pKa = 11.84FRR853 pKa = 11.84VCTHH857 pKa = 6.73PPIAPNPRR865 pKa = 11.84IGRR868 pKa = 11.84IQRR871 pKa = 11.84PGEE874 pKa = 3.82LVVEE878 pKa = 4.67DD879 pKa = 4.58EE880 pKa = 4.93CVHH883 pKa = 6.58TYY885 pKa = 7.75PTEE888 pKa = 4.07IFSFPYY894 pKa = 9.75VRR896 pKa = 11.84SRR898 pKa = 11.84TIDD901 pKa = 3.32PDD903 pKa = 3.98VFWEE907 pKa = 4.58SIAWAANQYY916 pKa = 11.11NSGCSSTHH924 pKa = 5.18

Molecular weight: 106.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJ26|A0A1L3KJ26_9VIRU Uncharacterized protein OS=Wenzhou narna-like virus 4 OX=1923579 PE=4 SV=1
MM1 pKa = 6.92TKK3 pKa = 10.07SRR5 pKa = 11.84RR6 pKa = 11.84GNTKK10 pKa = 9.54SGSRR14 pKa = 11.84PGTKK18 pKa = 8.95TKK20 pKa = 10.86AGLRR24 pKa = 11.84PAIAAKK30 pKa = 9.74IAKK33 pKa = 8.43PVKK36 pKa = 9.83AGRR39 pKa = 11.84FRR41 pKa = 11.84ALLNPGHH48 pKa = 6.45SPMEE52 pKa = 4.09HH53 pKa = 6.69ACAKK57 pKa = 10.32HH58 pKa = 4.14YY59 pKa = 7.68TQTVLDD65 pKa = 4.31PFNTEE70 pKa = 4.14AGACVPMLPCLDD82 pKa = 3.46SAKK85 pKa = 10.19RR86 pKa = 11.84KK87 pKa = 9.56VFARR91 pKa = 11.84GTGMTQSSNGFGGIVASTSVTKK113 pKa = 10.41DD114 pKa = 3.27APAVWYY120 pKa = 9.14TDD122 pKa = 3.25GSGTGDD128 pKa = 3.4TLTATHH134 pKa = 6.27NVAAYY139 pKa = 10.58SNSEE143 pKa = 4.19LVNTDD148 pKa = 3.07LTGGNVQARR157 pKa = 11.84IVGCGIRR164 pKa = 11.84LRR166 pKa = 11.84YY167 pKa = 9.02IGKK170 pKa = 9.69QIDD173 pKa = 3.32MSGRR177 pKa = 11.84TFALEE182 pKa = 4.18EE183 pKa = 4.58PNHH186 pKa = 6.52LNTINYY192 pKa = 7.52TVANCQALDD201 pKa = 3.38RR202 pKa = 11.84CKK204 pKa = 10.77SKK206 pKa = 10.84NLSRR210 pKa = 11.84EE211 pKa = 3.79WTVACWQPVRR221 pKa = 11.84PGEE224 pKa = 4.36QSYY227 pKa = 10.71QMNANAATDD236 pKa = 3.82PVHH239 pKa = 5.78YY240 pKa = 10.45QPLCVLIQAQAGQSLPFEE258 pKa = 4.76WEE260 pKa = 4.01WFCHH264 pKa = 5.75YY265 pKa = 10.44EE266 pKa = 4.3AIGYY270 pKa = 7.43GARR273 pKa = 11.84GKK275 pKa = 10.27SRR277 pKa = 11.84SHH279 pKa = 5.83VAPIAGPKK287 pKa = 9.96AIAALQQTPSDD298 pKa = 3.91VFDD301 pKa = 4.45DD302 pKa = 4.01VSNKK306 pKa = 9.75RR307 pKa = 11.84LSSGRR312 pKa = 11.84LADD315 pKa = 3.74NMISQGSSFDD325 pKa = 3.32WSKK328 pKa = 11.1LADD331 pKa = 3.32TGLRR335 pKa = 11.84VAQTAIGAVTSRR347 pKa = 11.84AAAQYY352 pKa = 9.9MSGGLAALALL362 pKa = 4.24

Molecular weight: 38.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1286

362

924

643.0

72.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.932 ± 2.602

2.411 ± 0.039

4.277 ± 0.069

5.754 ± 1.541

4.121 ± 0.842

6.454 ± 1.371

2.488 ± 0.143

4.821 ± 0.491

6.687 ± 0.883

7.854 ± 0.488

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.555 ± 0.337

3.81 ± 0.456

6.143 ± 0.603

4.432 ± 0.136

5.988 ± 0.096

7.154 ± 0.441

6.765 ± 0.357

5.677 ± 0.064

2.566 ± 0.468

3.11 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski