Virgibacillus indicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Virgibacillus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A265NBD5|A0A265NBD5_9BACI NADPH-dependent oxidoreductase OS=Virgibacillus indicus OX=2024554 GN=CIL03_06375 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.61KK3 pKa = 10.5KK4 pKa = 9.73MNCLILLLTIIFMVGCTNVEE24 pKa = 4.14DD25 pKa = 4.08TSNTDD30 pKa = 2.83VEE32 pKa = 4.48TDD34 pKa = 3.57SQEE37 pKa = 3.87VTAVEE42 pKa = 4.18TNKK45 pKa = 10.25NNEE48 pKa = 3.99KK49 pKa = 10.54EE50 pKa = 4.35EE51 pKa = 4.34TVTTVVDD58 pKa = 4.04EE59 pKa = 4.56PVVEE63 pKa = 4.41EE64 pKa = 4.39TPSAPVAEE72 pKa = 4.67EE73 pKa = 4.08TQTQPNNEE81 pKa = 4.13LFSGYY86 pKa = 8.92EE87 pKa = 4.0LIEE90 pKa = 3.96VDD92 pKa = 4.9GGDD95 pKa = 3.06LSGYY99 pKa = 9.69RR100 pKa = 11.84EE101 pKa = 4.23PNVVVDD107 pKa = 3.22IGYY110 pKa = 10.81GDD112 pKa = 3.54RR113 pKa = 11.84EE114 pKa = 3.97YY115 pKa = 10.27WAFTNEE121 pKa = 3.57HH122 pKa = 5.5GQLVRR127 pKa = 11.84VIADD131 pKa = 4.05EE132 pKa = 5.96IILQDD137 pKa = 4.09DD138 pKa = 3.73SNEE141 pKa = 3.95PVLSSGRR148 pKa = 11.84YY149 pKa = 8.18YY150 pKa = 10.94SDD152 pKa = 3.3EE153 pKa = 4.16AKK155 pKa = 10.96VPGVEE160 pKa = 4.89SDD162 pKa = 3.87VLDD165 pKa = 4.34EE166 pKa = 4.15GHH168 pKa = 7.25IIADD172 pKa = 3.69SLGGVSNAYY181 pKa = 10.43NITPQEE187 pKa = 4.08STLNRR192 pKa = 11.84HH193 pKa = 5.49GDD195 pKa = 3.36QAYY198 pKa = 8.88MEE200 pKa = 4.5DD201 pKa = 4.58AIRR204 pKa = 11.84SAGGATNFEE213 pKa = 4.55AIITYY218 pKa = 10.73SNTEE222 pKa = 3.83TQIPSSYY229 pKa = 10.51QYY231 pKa = 10.89TYY233 pKa = 10.69TLMGNEE239 pKa = 4.15IVDD242 pKa = 3.54TFDD245 pKa = 3.41NVNPDD250 pKa = 3.42EE251 pKa = 4.58VNASLGITDD260 pKa = 4.9SEE262 pKa = 4.59PSDD265 pKa = 3.56STSSNTNGDD274 pKa = 3.16ISSVDD279 pKa = 3.5TNGNGQVTIKK289 pKa = 9.64EE290 pKa = 4.21AKK292 pKa = 7.99EE293 pKa = 3.79AGYY296 pKa = 11.13SMPITSDD303 pKa = 2.52HH304 pKa = 6.18WLYY307 pKa = 10.63PYY309 pKa = 10.18MRR311 pKa = 11.84DD312 pKa = 3.38NDD314 pKa = 3.64NDD316 pKa = 3.78GMVGEE321 pKa = 4.62

Molecular weight: 35.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A265NBV0|A0A265NBV0_9BACI Transcriptional regulator OS=Virgibacillus indicus OX=2024554 GN=CIL03_07890 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 10.06NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3738

0

3738

1086022

27

1518

290.5

32.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.936 ± 0.043

0.62 ± 0.011

5.403 ± 0.034

7.806 ± 0.046

4.608 ± 0.036

6.761 ± 0.039

2.004 ± 0.017

8.286 ± 0.045

7.026 ± 0.039

9.492 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.832 ± 0.018

4.787 ± 0.029

3.478 ± 0.024

3.597 ± 0.03

3.811 ± 0.027

5.979 ± 0.024

5.309 ± 0.024

6.684 ± 0.037

1.008 ± 0.015

3.571 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski