Xinfangfangia humi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Xinfangfangia

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4199 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P5XEK8|A0A3P5XEK8_9RHOB Inner membrane protein YdcO OS=Xinfangfangia humi OX=2483812 GN=ydcO PE=4 SV=1
MM1 pKa = 7.16ATINGTAGNDD11 pKa = 3.59TLISGDD17 pKa = 3.96QADD20 pKa = 4.3TLNGLGGDD28 pKa = 3.75DD29 pKa = 3.58VLYY32 pKa = 11.09GGGGNDD38 pKa = 3.68SLSGGDD44 pKa = 4.98GNDD47 pKa = 3.54LLYY50 pKa = 11.22GGDD53 pKa = 4.8GNDD56 pKa = 3.8TLNGGNGNDD65 pKa = 3.94TLIGGPGADD74 pKa = 3.88SLIGGAGIDD83 pKa = 3.57TVDD86 pKa = 3.54YY87 pKa = 9.8SGSGAAVNVNLGSSSAATGGDD108 pKa = 3.46AQGDD112 pKa = 3.97RR113 pKa = 11.84YY114 pKa = 10.98SGIEE118 pKa = 3.8IVIGSAFNDD127 pKa = 4.54TITGDD132 pKa = 3.31TGANTLSGGLGNDD145 pKa = 3.25QLYY148 pKa = 10.69GGAGADD154 pKa = 3.5VLDD157 pKa = 4.55GGEE160 pKa = 4.8GNDD163 pKa = 3.59SLYY166 pKa = 11.17GGSGNDD172 pKa = 3.62TLIGGAGADD181 pKa = 3.97YY182 pKa = 11.08LDD184 pKa = 4.86GGADD188 pKa = 3.82SDD190 pKa = 4.24TVDD193 pKa = 3.74YY194 pKa = 10.8SGSDD198 pKa = 2.93AAVNVNLTSGSGSGGHH214 pKa = 6.16AQGDD218 pKa = 4.05TYY220 pKa = 11.88VNIEE224 pKa = 4.56NITGSDD230 pKa = 3.72YY231 pKa = 11.76DD232 pKa = 4.49DD233 pKa = 3.84VLIGNSGANILSGGAGNDD251 pKa = 3.27SLYY254 pKa = 11.29GGGGNDD260 pKa = 3.28SLYY263 pKa = 11.28GGDD266 pKa = 5.58GDD268 pKa = 5.68DD269 pKa = 5.6LLDD272 pKa = 4.68GGDD275 pKa = 4.63GNDD278 pKa = 3.56SLYY281 pKa = 11.22GGNGNDD287 pKa = 3.82TLIGGAGSDD296 pKa = 3.98RR297 pKa = 11.84LDD299 pKa = 3.58GGAGIDD305 pKa = 3.55TVDD308 pKa = 3.59YY309 pKa = 10.77SGSTSGVNVNLSTGSGSSGHH329 pKa = 6.78AAGDD333 pKa = 3.74TYY335 pKa = 11.69VSIEE339 pKa = 4.0NVIGSAYY346 pKa = 10.51NDD348 pKa = 4.31TIVGTAGANYY358 pKa = 10.13LSGGAGRR365 pKa = 11.84DD366 pKa = 3.83SISGGDD372 pKa = 4.08GDD374 pKa = 4.44DD375 pKa = 3.81TLAGGAGADD384 pKa = 4.06TLNGGQGLDD393 pKa = 2.88IVDD396 pKa = 3.96YY397 pKa = 10.58SEE399 pKa = 5.04SDD401 pKa = 3.09AAVNINLSTWTASGGHH417 pKa = 5.47ATGDD421 pKa = 3.68VLSGIDD427 pKa = 4.38GIIGSAFDD435 pKa = 4.04DD436 pKa = 3.75VLIGFNEE443 pKa = 4.25MGTGADD449 pKa = 3.18SYY451 pKa = 11.4TNVIYY456 pKa = 10.59GGAGNDD462 pKa = 4.13TIDD465 pKa = 4.55GMGGDD470 pKa = 4.1DD471 pKa = 3.96SLYY474 pKa = 10.9GGTGNDD480 pKa = 3.5YY481 pKa = 11.52VLGGSGNDD489 pKa = 3.16TVYY492 pKa = 11.2GDD494 pKa = 3.84EE495 pKa = 4.59GDD497 pKa = 3.7DD498 pKa = 3.23RR499 pKa = 11.84VYY501 pKa = 11.13GGSGDD506 pKa = 4.2DD507 pKa = 3.88LVSGGSGNDD516 pKa = 3.06QVYY519 pKa = 10.77GDD521 pKa = 4.77EE522 pKa = 4.68GTDD525 pKa = 3.19TLYY528 pKa = 11.25GDD530 pKa = 4.06EE531 pKa = 5.04GNDD534 pKa = 3.28VLYY537 pKa = 11.02GGTGNDD543 pKa = 3.93LLYY546 pKa = 11.19GGADD550 pKa = 3.57EE551 pKa = 4.53DD552 pKa = 4.18TLYY555 pKa = 11.36GGDD558 pKa = 4.52GVDD561 pKa = 3.79TLHH564 pKa = 7.13GDD566 pKa = 3.44SGNDD570 pKa = 3.43SLFGDD575 pKa = 4.51AGNDD579 pKa = 3.5LLYY582 pKa = 11.04GGTGDD587 pKa = 3.51DD588 pKa = 3.76TLYY591 pKa = 11.28GGAGNDD597 pKa = 3.4QLFGGDD603 pKa = 3.57GRR605 pKa = 11.84DD606 pKa = 3.48VLHH609 pKa = 7.49ADD611 pKa = 4.14GGNDD615 pKa = 3.42TLTGGAGNDD624 pKa = 3.46VFVVYY629 pKa = 9.9PGSGAVLITDD639 pKa = 4.28FGAGNTGPIDD649 pKa = 5.67DD650 pKa = 5.87DD651 pKa = 5.61DD652 pKa = 3.92NTNNDD657 pKa = 3.86FVDD660 pKa = 4.73LSAWYY665 pKa = 10.74NEE667 pKa = 4.0TTLAAWNAANPGQTYY682 pKa = 10.74NNPLQWMIADD692 pKa = 3.98HH693 pKa = 7.41ADD695 pKa = 3.29GVLNAVGGGLRR706 pKa = 11.84IQNGGVAVSSDD717 pKa = 3.18MLVFEE722 pKa = 4.65TTGVVCFARR731 pKa = 11.84GTRR734 pKa = 11.84ILTPRR739 pKa = 11.84GEE741 pKa = 4.28IAVEE745 pKa = 3.61ALRR748 pKa = 11.84AGDD751 pKa = 4.9LVTTLDD757 pKa = 4.2AGPQVLRR764 pKa = 11.84WIGSRR769 pKa = 11.84RR770 pKa = 11.84IGAAEE775 pKa = 4.05LAADD779 pKa = 4.33PGLIPVRR786 pKa = 11.84IRR788 pKa = 11.84AGSLGQGLPRR798 pKa = 11.84RR799 pKa = 11.84DD800 pKa = 3.37LTVSPQHH807 pKa = 6.83RR808 pKa = 11.84ILLRR812 pKa = 11.84SRR814 pKa = 11.84IVEE817 pKa = 4.04RR818 pKa = 11.84MTGEE822 pKa = 4.2PEE824 pKa = 3.72VLLAAKK830 pKa = 10.32HH831 pKa = 4.28MTGLDD836 pKa = 3.38GLEE839 pKa = 4.1VVGDD843 pKa = 4.01CRR845 pKa = 11.84GVEE848 pKa = 4.13YY849 pKa = 10.36FHH851 pKa = 8.04LLFDD855 pKa = 3.75RR856 pKa = 11.84HH857 pKa = 6.11QILFAEE863 pKa = 5.3GAPAEE868 pKa = 4.13SLLFGKK874 pKa = 8.27EE875 pKa = 3.57ARR877 pKa = 11.84KK878 pKa = 8.48MLRR881 pKa = 11.84PAAVEE886 pKa = 4.29EE887 pKa = 4.26ILTVLPALAVAPPPPARR904 pKa = 11.84ALMDD908 pKa = 3.43GRR910 pKa = 11.84TGRR913 pKa = 11.84SLVRR917 pKa = 11.84RR918 pKa = 11.84MRR920 pKa = 11.84QNGVEE925 pKa = 4.35AICAAEE931 pKa = 4.14APPVRR936 pKa = 11.84VTAA939 pKa = 4.38

Molecular weight: 93.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P5WSR9|A0A3P5WSR9_9RHOB Sarcosine oxidase gamma subunit family OS=Xinfangfangia humi OX=2483812 GN=XINFAN_01672 PE=4 SV=1
MM1 pKa = 7.36RR2 pKa = 11.84RR3 pKa = 11.84VRR5 pKa = 11.84MGAAPRR11 pKa = 11.84APRR14 pKa = 11.84PLWKK18 pKa = 10.22RR19 pKa = 11.84LGWFALIWALSVAALGLVALVFRR42 pKa = 11.84LMMKK46 pKa = 10.6LAGLSLL52 pKa = 3.8

Molecular weight: 5.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4199

0

4199

1295700

29

2121

308.6

33.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.275 ± 0.058

0.881 ± 0.01

5.526 ± 0.031

5.918 ± 0.032

3.566 ± 0.023

9.217 ± 0.038

1.996 ± 0.018

4.957 ± 0.025

2.683 ± 0.031

10.502 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.018

2.261 ± 0.021

5.548 ± 0.032

2.949 ± 0.019

7.605 ± 0.043

4.97 ± 0.024

5.03 ± 0.026

6.976 ± 0.031

1.504 ± 0.016

2.035 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski