Leclercia phage 10164RH

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teetrevirus; unclassified Teetrevirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A289Z6T9|A0A289Z6T9_9CAUD Uncharacterized protein OS=Leclercia phage 10164RH OX=2024249 GN=10164RH_00036 PE=4 SV=1
MM1 pKa = 7.73IMPKK5 pKa = 10.08SDD7 pKa = 3.6TVTMTRR13 pKa = 11.84DD14 pKa = 2.64AWNDD18 pKa = 2.85VSAYY22 pKa = 9.32IDD24 pKa = 3.7KK25 pKa = 11.04LEE27 pKa = 4.85KK28 pKa = 10.7DD29 pKa = 4.0LEE31 pKa = 4.14FLNALKK37 pKa = 10.74ACGVDD42 pKa = 3.06NWDD45 pKa = 3.98GYY47 pKa = 10.81SDD49 pKa = 4.02AVEE52 pKa = 4.1MVYY55 pKa = 11.01GEE57 pKa = 4.71DD58 pKa = 4.8DD59 pKa = 3.52EE60 pKa = 5.21

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A289ZST5|A0A289ZST5_9CAUD Single-stranded DNA-binding protein OS=Leclercia phage 10164RH OX=2024249 GN=10164RH_00014 PE=4 SV=1
MM1 pKa = 6.77MAALIALVYY10 pKa = 10.28LCIAMYY16 pKa = 9.72FMRR19 pKa = 11.84DD20 pKa = 3.63FKK22 pKa = 11.05RR23 pKa = 11.84GLTIHH28 pKa = 7.19KK29 pKa = 8.14ATLSYY34 pKa = 9.75FKK36 pKa = 10.03WGFIPRR42 pKa = 11.84FTVRR46 pKa = 11.84EE47 pKa = 3.73RR48 pKa = 11.84NGRR51 pKa = 11.84FKK53 pKa = 11.15ANKK56 pKa = 9.51VGVFYY61 pKa = 10.74IATHH65 pKa = 5.88

Molecular weight: 7.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11729

35

1320

255.0

28.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.54 ± 0.433

0.793 ± 0.131

6.267 ± 0.24

7.162 ± 0.441

3.666 ± 0.242

7.614 ± 0.339

1.918 ± 0.18

5.098 ± 0.213

6.573 ± 0.419

8.014 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.848 ± 0.216

4.502 ± 0.352

3.683 ± 0.232

4.144 ± 0.305

5.806 ± 0.21

5.729 ± 0.327

5.243 ± 0.239

6.514 ± 0.354

1.543 ± 0.146

3.342 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski