Apibacter muscae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Apibacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2183 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A563D964|A0A563D964_9FLAO Glyceraldehyde-3-phosphate dehydrogenase OS=Apibacter muscae OX=2509004 GN=gap PE=3 SV=1
MM1 pKa = 7.53SIIITDD7 pKa = 3.39EE8 pKa = 4.54CINCGACEE16 pKa = 4.03PEE18 pKa = 4.51CPNNAIYY25 pKa = 10.44EE26 pKa = 4.38GAVDD30 pKa = 3.65WKK32 pKa = 10.59MSDD35 pKa = 3.27GTDD38 pKa = 3.47LSGHH42 pKa = 6.5VITPSGLSIDD52 pKa = 4.27ADD54 pKa = 3.79APQKK58 pKa = 10.51PISDD62 pKa = 3.61DD63 pKa = 3.75VYY65 pKa = 11.05FIVSDD70 pKa = 3.66KK71 pKa = 10.53CTEE74 pKa = 3.72CHH76 pKa = 6.44GFHH79 pKa = 7.45EE80 pKa = 5.22EE81 pKa = 4.04PQCAAVCPVDD91 pKa = 4.87CCIPDD96 pKa = 4.34DD97 pKa = 3.87NHH99 pKa = 7.59KK100 pKa = 9.58EE101 pKa = 3.97TDD103 pKa = 3.39EE104 pKa = 4.0QLLSKK109 pKa = 10.88KK110 pKa = 10.41NFLHH114 pKa = 6.7SS115 pKa = 3.57

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A563DCU2|A0A563DCU2_9FLAO TROVE domain-containing protein OS=Apibacter muscae OX=2509004 GN=ETU09_06550 PE=3 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84IVNNFEE22 pKa = 4.71EE23 pKa = 4.61ITTSKK28 pKa = 10.54PEE30 pKa = 3.65KK31 pKa = 10.2SLLRR35 pKa = 11.84KK36 pKa = 9.27ISKK39 pKa = 10.33SGGRR43 pKa = 11.84NNSGKK48 pKa = 8.25MTMRR52 pKa = 11.84YY53 pKa = 7.81TGGGHH58 pKa = 6.19KK59 pKa = 9.81KK60 pKa = 9.9RR61 pKa = 11.84YY62 pKa = 9.52RR63 pKa = 11.84EE64 pKa = 3.64IDD66 pKa = 3.38FKK68 pKa = 11.17RR69 pKa = 11.84NKK71 pKa = 9.89FGIPATVKK79 pKa = 10.48SVEE82 pKa = 3.91YY83 pKa = 10.55DD84 pKa = 3.39PNRR87 pKa = 11.84TAFIALLVYY96 pKa = 10.85ADD98 pKa = 3.54GEE100 pKa = 4.04KK101 pKa = 10.23RR102 pKa = 11.84YY103 pKa = 9.91IVAPHH108 pKa = 7.09GIKK111 pKa = 10.0VDD113 pKa = 3.51QKK115 pKa = 10.73VISGDD120 pKa = 3.42TVAPEE125 pKa = 3.9IGNAMKK131 pKa = 10.68LKK133 pKa = 10.01NIPLGTVVSCIEE145 pKa = 3.92LRR147 pKa = 11.84PGQGAKK153 pKa = 8.51MARR156 pKa = 11.84SAGTSAQLIARR167 pKa = 11.84DD168 pKa = 3.38GKK170 pKa = 10.41YY171 pKa = 10.39AVLKK175 pKa = 9.42MPSGEE180 pKa = 3.74TRR182 pKa = 11.84MILVEE187 pKa = 4.2CLATIGSVSNSDD199 pKa = 3.16HH200 pKa = 6.15QLTVSGKK207 pKa = 9.29AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.0GRR238 pKa = 11.84STGGHH243 pKa = 5.23PRR245 pKa = 11.84SRR247 pKa = 11.84KK248 pKa = 9.34GMPAKK253 pKa = 10.42GFKK256 pKa = 9.23TRR258 pKa = 11.84SKK260 pKa = 9.88KK261 pKa = 10.3KK262 pKa = 9.09EE263 pKa = 4.13SNRR266 pKa = 11.84HH267 pKa = 4.32ILQKK271 pKa = 10.7RR272 pKa = 11.84KK273 pKa = 9.95

Molecular weight: 30.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2183

0

2183

719535

38

3445

329.6

37.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.185 ± 0.047

0.831 ± 0.017

5.021 ± 0.037

6.833 ± 0.051

5.179 ± 0.044

5.929 ± 0.047

1.655 ± 0.02

9.258 ± 0.055

8.822 ± 0.053

9.769 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.044 ± 0.025

6.642 ± 0.051

3.357 ± 0.031

3.517 ± 0.031

3.126 ± 0.035

6.775 ± 0.04

5.113 ± 0.034

5.469 ± 0.036

0.984 ± 0.019

4.492 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski