Paenibacillus graminis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5576 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A089M4J0|A0A089M4J0_9BACL Uncharacterized protein OS=Paenibacillus graminis OX=189425 GN=PGRAT_11265 PE=4 SV=1
MM1 pKa = 7.2NVYY4 pKa = 9.68KK5 pKa = 11.06VNEE8 pKa = 3.76YY9 pKa = 9.85WIAAKK14 pKa = 9.25DD15 pKa = 3.18TDD17 pKa = 4.06AAFGQYY23 pKa = 10.47LEE25 pKa = 4.66EE26 pKa = 4.31TDD28 pKa = 4.01SLEE31 pKa = 4.19TMIVDD36 pKa = 4.19DD37 pKa = 4.29LAEE40 pKa = 4.4GEE42 pKa = 4.26EE43 pKa = 4.45TEE45 pKa = 3.96ITVSIKK51 pKa = 10.64RR52 pKa = 11.84LTTKK56 pKa = 10.32EE57 pKa = 4.16IEE59 pKa = 4.5TQTVPCCEE67 pKa = 4.79DD68 pKa = 3.3GCDD71 pKa = 3.75RR72 pKa = 11.84CEE74 pKa = 4.56DD75 pKa = 4.21LNDD78 pKa = 4.01HH79 pKa = 7.16LYY81 pKa = 9.36DD82 pKa = 4.15TYY84 pKa = 11.5QEE86 pKa = 4.99LLTQRR91 pKa = 11.84TDD93 pKa = 4.09FPCVLAKK100 pKa = 10.5EE101 pKa = 4.35II102 pKa = 4.21

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A089MF20|A0A089MF20_9BACL Alpha-mannosidase OS=Paenibacillus graminis OX=189425 GN=PGRAT_22340 PE=3 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5576

0

5576

1797145

26

3072

322.3

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.625 ± 0.038

0.785 ± 0.008

4.928 ± 0.027

6.695 ± 0.036

4.111 ± 0.025

7.757 ± 0.033

2.046 ± 0.014

6.55 ± 0.035

5.168 ± 0.028

10.449 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.018

3.758 ± 0.024

4.129 ± 0.022

3.8 ± 0.021

5.011 ± 0.03

6.429 ± 0.025

5.316 ± 0.03

6.906 ± 0.027

1.215 ± 0.014

3.554 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski