Modestobacter sp. DSM 44400

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Modestobacter; unclassified Modestobacter

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5140 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3DL19|A0A1H3DL19_9ACTN Uncharacterized protein OS=Modestobacter sp. DSM 44400 OX=1550230 GN=SAMN05661080_00770 PE=4 SV=1
MM1 pKa = 7.82PSPSRR6 pKa = 11.84RR7 pKa = 11.84LLAAVVVVSIAALPVLAGCGVTFVVGSASPATGQVGTVQSQDD49 pKa = 3.31FPVIGATEE57 pKa = 4.07DD58 pKa = 4.48EE59 pKa = 4.25IDD61 pKa = 3.53QLARR65 pKa = 11.84NALVDD70 pKa = 6.0LNVFWQNQFPDD81 pKa = 3.34TFGQEE86 pKa = 4.06FTPLRR91 pKa = 11.84GGYY94 pKa = 10.25YY95 pKa = 10.35SVDD98 pKa = 3.64PANIDD103 pKa = 3.2PTQYY107 pKa = 10.3PGGQIGCGEE116 pKa = 4.2TPDD119 pKa = 4.31AVEE122 pKa = 5.72DD123 pKa = 3.69NAFYY127 pKa = 10.75CPPSNEE133 pKa = 4.22YY134 pKa = 10.38PNGDD138 pKa = 3.94AIQYY142 pKa = 10.28DD143 pKa = 3.93RR144 pKa = 11.84AFLQDD149 pKa = 3.64LANEE153 pKa = 4.26YY154 pKa = 11.16GRR156 pKa = 11.84FLPALVMAHH165 pKa = 6.3EE166 pKa = 5.34FGHH169 pKa = 7.21AIQGRR174 pKa = 11.84VGYY177 pKa = 8.71PLQASIAIEE186 pKa = 4.03TQADD190 pKa = 3.98CFAGAWTAWVADD202 pKa = 3.86GNAPHH207 pKa = 5.84TTIRR211 pKa = 11.84TPEE214 pKa = 4.36LDD216 pKa = 3.62DD217 pKa = 3.56VLRR220 pKa = 11.84GYY222 pKa = 11.18LLLRR226 pKa = 11.84DD227 pKa = 4.03PVGTDD232 pKa = 3.31LNEE235 pKa = 4.38SEE237 pKa = 4.52AHH239 pKa = 6.15GSLFDD244 pKa = 3.77RR245 pKa = 11.84VSAFQQGFDD254 pKa = 4.79DD255 pKa = 5.89GPTACRR261 pKa = 11.84DD262 pKa = 3.22EE263 pKa = 4.9FGADD267 pKa = 3.14RR268 pKa = 11.84IYY270 pKa = 10.04TQRR273 pKa = 11.84EE274 pKa = 3.83FDD276 pKa = 3.84PADD279 pKa = 3.5AATQGNASYY288 pKa = 10.3PVTQQLVTEE297 pKa = 4.34SLSTFWSRR305 pKa = 11.84TFTARR310 pKa = 11.84GEE312 pKa = 4.22TFAAPDD318 pKa = 3.55VVPFSGTAPDD328 pKa = 4.45CAGQGNEE335 pKa = 3.82QADD338 pKa = 4.13LVFCANKK345 pKa = 9.2DD346 pKa = 3.52TVGYY350 pKa = 10.87DD351 pKa = 3.07EE352 pKa = 5.02TDD354 pKa = 3.1LVRR357 pKa = 11.84PVYY360 pKa = 10.55EE361 pKa = 4.04EE362 pKa = 4.33LGDD365 pKa = 3.95YY366 pKa = 10.99AVLTAVAIPYY376 pKa = 10.18SLAARR381 pKa = 11.84DD382 pKa = 3.82QLGLSTDD389 pKa = 3.71DD390 pKa = 3.14QDD392 pKa = 3.89AARR395 pKa = 11.84SAVCLTGAFTRR406 pKa = 11.84SVLSGEE412 pKa = 4.4VNGITISPGDD422 pKa = 3.81ADD424 pKa = 3.82EE425 pKa = 4.98SVQFLLGYY433 pKa = 10.29SDD435 pKa = 5.43DD436 pKa = 4.43PDD438 pKa = 3.59VLGDD442 pKa = 3.5VGLSGFQLVDD452 pKa = 3.04VFRR455 pKa = 11.84SGVVEE460 pKa = 4.36GEE462 pKa = 4.31SSCGIGG468 pKa = 3.84

Molecular weight: 49.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3LTL8|A0A1H3LTL8_9ACTN Endoglucanase OS=Modestobacter sp. DSM 44400 OX=1550230 GN=SAMN05661080_04249 PE=3 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84RR3 pKa = 11.84TPAGAVPAVRR13 pKa = 11.84PVPVPGRR20 pKa = 11.84RR21 pKa = 11.84PPSRR25 pKa = 11.84SRR27 pKa = 11.84PPRR30 pKa = 11.84SPRR33 pKa = 11.84LRR35 pKa = 11.84SRR37 pKa = 11.84RR38 pKa = 11.84PRR40 pKa = 11.84SPRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84SPRR48 pKa = 11.84RR49 pKa = 11.84PRR51 pKa = 11.84LRR53 pKa = 11.84RR54 pKa = 11.84LRR56 pKa = 11.84RR57 pKa = 11.84WLPRR61 pKa = 11.84PIVQPHH67 pKa = 6.04RR68 pKa = 11.84ARR70 pKa = 11.84ARR72 pKa = 11.84GRR74 pKa = 11.84VRR76 pKa = 11.84PPRR79 pKa = 11.84HH80 pKa = 6.06RR81 pKa = 11.84PPPSRR86 pKa = 11.84SRR88 pKa = 11.84LPSSRR93 pKa = 11.84LCSSRR98 pKa = 11.84RR99 pKa = 11.84PARR102 pKa = 11.84LSSSPRR108 pKa = 11.84PRR110 pKa = 11.84LSRR113 pKa = 11.84VRR115 pKa = 11.84VRR117 pKa = 11.84SGRR120 pKa = 11.84VRR122 pKa = 11.84PGAPPPVLRR131 pKa = 11.84VRR133 pKa = 11.84LVPVRR138 pKa = 11.84VPAPGRR144 pKa = 11.84RR145 pKa = 11.84RR146 pKa = 11.84VRR148 pKa = 11.84ATTPSAVPRR157 pKa = 11.84VRR159 pKa = 11.84PRR161 pKa = 11.84RR162 pKa = 11.84AARR165 pKa = 11.84LLPVRR170 pKa = 11.84ALPVRR175 pKa = 11.84ALPVRR180 pKa = 11.84ALPVRR185 pKa = 11.84ALPVRR190 pKa = 11.84VRR192 pKa = 11.84LARR195 pKa = 11.84AVPVPARR202 pKa = 11.84VPVDD206 pKa = 3.45LVRR209 pKa = 11.84RR210 pKa = 11.84PGPVDD215 pKa = 3.44LVRR218 pKa = 11.84RR219 pKa = 11.84PGPVDD224 pKa = 3.81LVPRR228 pKa = 11.84PAPVDD233 pKa = 3.59LVLPPVRR240 pKa = 11.84VGPGPTRR247 pKa = 11.84GG248 pKa = 3.31

Molecular weight: 27.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5140

0

5140

1452836

39

2006

282.7

29.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.334 ± 0.053

0.745 ± 0.011

6.144 ± 0.032

5.159 ± 0.029

2.533 ± 0.018

9.65 ± 0.034

2.078 ± 0.017

2.858 ± 0.025

1.439 ± 0.025

10.629 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.666 ± 0.012

1.516 ± 0.017

6.179 ± 0.032

2.856 ± 0.02

8.077 ± 0.036

5.156 ± 0.029

6.128 ± 0.026

9.603 ± 0.039

1.471 ± 0.014

1.779 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski