Aminicella lysinilytica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Aminicella

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2119 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6QC96|A0A4R6QC96_9FIRM tRNA modification GTPase MnmE OS=Aminicella lysinilytica OX=433323 GN=mnmE PE=3 SV=1
MM1 pKa = 8.24DD2 pKa = 4.21FTFISQMFIPIVLAACLVIGYY23 pKa = 8.76IVKK26 pKa = 10.33HH27 pKa = 5.38VLPTNNKK34 pKa = 8.5WIPLILAVSGALLGCWSNANIEE56 pKa = 4.34LTSVVYY62 pKa = 10.12GAVTGLASTGMYY74 pKa = 9.93EE75 pKa = 4.95AFTQFIEE82 pKa = 4.7SKK84 pKa = 10.53NSYY87 pKa = 10.69NSGDD91 pKa = 3.75DD92 pKa = 3.47SKK94 pKa = 11.72GFPSEE99 pKa = 3.74TAAADD104 pKa = 3.49INNATSAAAVEE115 pKa = 4.1NEE117 pKa = 3.9EE118 pKa = 5.52AIDD121 pKa = 4.23AEE123 pKa = 5.2AGDD126 pKa = 4.09SDD128 pKa = 4.62VNN130 pKa = 3.65

Molecular weight: 13.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6QAY0|A0A4R6QAY0_9FIRM D-ornithine 4 5-aminomutase E subunit OS=Aminicella lysinilytica OX=433323 GN=EV211_1053 PE=4 SV=1
MM1 pKa = 7.32LRR3 pKa = 11.84KK4 pKa = 9.55NVLRR8 pKa = 11.84YY9 pKa = 9.9QKK11 pKa = 10.75DD12 pKa = 2.74FDD14 pKa = 4.91AIYY17 pKa = 10.58RR18 pKa = 11.84KK19 pKa = 9.97GKK21 pKa = 9.83SVGDD25 pKa = 3.34RR26 pKa = 11.84FVVVFCKK33 pKa = 10.65KK34 pKa = 10.34NGLSYY39 pKa = 10.87GRR41 pKa = 11.84RR42 pKa = 11.84AFLASKK48 pKa = 10.44KK49 pKa = 10.25VGNSVQRR56 pKa = 11.84NRR58 pKa = 11.84ARR60 pKa = 11.84RR61 pKa = 11.84LMKK64 pKa = 9.76EE65 pKa = 4.15AYY67 pKa = 8.61RR68 pKa = 11.84LSSIEE73 pKa = 4.73LPDD76 pKa = 4.51GYY78 pKa = 10.84DD79 pKa = 3.29FVIIARR85 pKa = 11.84NTIGDD90 pKa = 4.24ANCQMVQRR98 pKa = 11.84SLRR101 pKa = 11.84SALKK105 pKa = 9.25RR106 pKa = 11.84TGVLSKK112 pKa = 11.14

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2119

0

2119

673308

29

2423

317.7

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.842 ± 0.056

1.492 ± 0.024

6.398 ± 0.043

6.74 ± 0.058

3.887 ± 0.034

7.519 ± 0.058

1.63 ± 0.022

7.591 ± 0.05

7.206 ± 0.054

8.215 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.288 ± 0.026

4.422 ± 0.036

3.203 ± 0.026

2.581 ± 0.028

4.331 ± 0.039

6.098 ± 0.056

5.695 ± 0.058

7.059 ± 0.033

0.791 ± 0.017

4.01 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski