HCBI8.215 virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus bovas1; Bovine associated gemykibivirus 1

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077XLY1|A0A077XLY1_9VIRU Replication associated protein OS=HCBI8.215 virus OX=1516079 GN=Rep PE=3 SV=1
MM1 pKa = 6.81STFRR5 pKa = 11.84FYY7 pKa = 11.55ARR9 pKa = 11.84YY10 pKa = 9.84ALLTYY15 pKa = 8.76AQCGGLDD22 pKa = 3.42PFTIVSHH29 pKa = 6.94IGDD32 pKa = 4.26LGGEE36 pKa = 4.27CIIGRR41 pKa = 11.84EE42 pKa = 4.08AHH44 pKa = 6.42SDD46 pKa = 3.43GGTHH50 pKa = 6.34LHH52 pKa = 6.56AFVDD56 pKa = 4.72FGRR59 pKa = 11.84RR60 pKa = 11.84FQSRR64 pKa = 11.84RR65 pKa = 11.84AAVFDD70 pKa = 3.38VGGFHH75 pKa = 7.23PNISITKK82 pKa = 9.17RR83 pKa = 11.84DD84 pKa = 3.72PQVHH88 pKa = 6.21YY89 pKa = 10.59DD90 pKa = 3.67YY91 pKa = 10.85AIKK94 pKa = 10.96DD95 pKa = 3.58GDD97 pKa = 4.01VVAGGLEE104 pKa = 4.07RR105 pKa = 11.84PAADD109 pKa = 3.1ASAGVEE115 pKa = 4.2NKK117 pKa = 9.24HH118 pKa = 5.67HH119 pKa = 6.42QIVLAPTRR127 pKa = 11.84AEE129 pKa = 3.7FFEE132 pKa = 4.3AVRR135 pKa = 11.84DD136 pKa = 3.68LDD138 pKa = 3.68PRR140 pKa = 11.84LLLTSFPSLEE150 pKa = 4.79KK151 pKa = 10.83YY152 pKa = 10.75ADD154 pKa = 2.59WHH156 pKa = 6.06YY157 pKa = 11.17RR158 pKa = 11.84VDD160 pKa = 4.05PDD162 pKa = 4.5PYY164 pKa = 10.56RR165 pKa = 11.84HH166 pKa = 6.22DD167 pKa = 3.79PEE169 pKa = 5.17YY170 pKa = 11.05EE171 pKa = 3.74FDD173 pKa = 3.34TSQFPEE179 pKa = 4.12LNQWAEE185 pKa = 4.86DD186 pKa = 4.09YY187 pKa = 10.71IGWDD191 pKa = 3.05ATVRR195 pKa = 11.84GG196 pKa = 4.08

Molecular weight: 22.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077XLY1|A0A077XLY1_9VIRU Replication associated protein OS=HCBI8.215 virus OX=1516079 GN=Rep PE=3 SV=1
MM1 pKa = 7.76AFRR4 pKa = 11.84NRR6 pKa = 11.84RR7 pKa = 11.84FQAGRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84FTPRR19 pKa = 11.84RR20 pKa = 11.84STFRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84SFRR29 pKa = 11.84NGRR32 pKa = 11.84SRR34 pKa = 11.84TTRR37 pKa = 11.84RR38 pKa = 11.84YY39 pKa = 9.42SRR41 pKa = 11.84PRR43 pKa = 11.84TNVRR47 pKa = 11.84RR48 pKa = 11.84VRR50 pKa = 11.84NLASRR55 pKa = 11.84KK56 pKa = 9.58CRR58 pKa = 11.84DD59 pKa = 3.3NMICTPIDD67 pKa = 3.5DD68 pKa = 4.45TGTPQTPGPVSMDD81 pKa = 3.02ASSVYY86 pKa = 10.76GFIFSPTARR95 pKa = 11.84TAGTQLEE102 pKa = 4.34SGEE105 pKa = 4.35RR106 pKa = 11.84VNAPSSAQRR115 pKa = 11.84RR116 pKa = 11.84KK117 pKa = 9.22SKK119 pKa = 10.67CYY121 pKa = 8.68VRR123 pKa = 11.84GYY125 pKa = 10.86GEE127 pKa = 4.14TVEE130 pKa = 4.56IQTDD134 pKa = 3.94DD135 pKa = 3.29ASPWVWRR142 pKa = 11.84RR143 pKa = 11.84IVFSTIGLASQFDD156 pKa = 3.87PGLLYY161 pKa = 11.27NNDD164 pKa = 3.46EE165 pKa = 4.1TRR167 pKa = 11.84GWGRR171 pKa = 11.84SLFNIRR177 pKa = 11.84SGTQQASILEE187 pKa = 4.34GVVFRR192 pKa = 11.84YY193 pKa = 9.89LFQGTEE199 pKa = 4.11GVDD202 pKa = 3.09WANVMTATTAPRR214 pKa = 11.84HH215 pKa = 4.66VRR217 pKa = 11.84VYY219 pKa = 9.64SDD221 pKa = 3.29RR222 pKa = 11.84RR223 pKa = 11.84RR224 pKa = 11.84VIQSPNSEE232 pKa = 3.9GGTIRR237 pKa = 11.84LSKK240 pKa = 10.56HH241 pKa = 5.14YY242 pKa = 10.64DD243 pKa = 3.66GINRR247 pKa = 11.84SIIYY251 pKa = 10.32DD252 pKa = 3.65DD253 pKa = 4.14VEE255 pKa = 4.71IGAGAKK261 pKa = 7.94ATNAIASGTPYY272 pKa = 11.37GNMGDD277 pKa = 4.75LFVLDD282 pKa = 4.95FFSSASDD289 pKa = 4.03DD290 pKa = 3.95EE291 pKa = 4.57TSGASFQPHH300 pKa = 5.72GKK302 pKa = 9.87FYY304 pKa = 9.48WHH306 pKa = 7.28EE307 pKa = 4.29GQSAA311 pKa = 3.13

Molecular weight: 35.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

611

104

311

203.7

23.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.692 ± 0.924

1.146 ± 0.206

7.201 ± 1.186

4.583 ± 0.643

6.056 ± 0.363

8.511 ± 0.476

2.455 ± 1.168

4.91 ± 0.242

3.601 ± 2.109

5.237 ± 1.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.309 ± 0.359

3.273 ± 0.834

4.746 ± 0.406

3.437 ± 0.259

9.493 ± 2.709

7.692 ± 1.514

6.219 ± 1.119

5.728 ± 0.268

2.128 ± 0.714

4.583 ± 0.696
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski