Myceligenerans xiligouense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Promicromonosporaceae; Myceligenerans

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3975 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N4ZCG6|A0A3N4ZCG6_9MICO Type II secretion system (T2SS) protein F OS=Myceligenerans xiligouense OX=253184 GN=EDD34_3843 PE=4 SV=1
MM1 pKa = 6.54QTRR4 pKa = 11.84KK5 pKa = 9.15QRR7 pKa = 11.84GRR9 pKa = 11.84PMAAAATALALAGVVLSPVTAQAEE33 pKa = 4.41DD34 pKa = 3.78GTEE37 pKa = 3.99LLTNPTFDD45 pKa = 3.69GTLDD49 pKa = 3.53PWWASEE55 pKa = 3.93GVEE58 pKa = 4.31FTEE61 pKa = 5.62APDD64 pKa = 3.61GTLCGVVPGGGNQWDD79 pKa = 4.29VIVGQGGVPVTAGDD93 pKa = 3.7TFTISVTAAASPEE106 pKa = 3.5ASARR110 pKa = 11.84VMVPDD115 pKa = 5.73PEE117 pKa = 4.73TEE119 pKa = 4.01WPPLYY124 pKa = 10.7YY125 pKa = 10.66ADD127 pKa = 3.2ITLRR131 pKa = 11.84ADD133 pKa = 3.29GQTFTEE139 pKa = 4.67AFDD142 pKa = 3.45IAADD146 pKa = 3.8AEE148 pKa = 4.84SEE150 pKa = 4.36VQLQLGGNAEE160 pKa = 4.15DD161 pKa = 5.85FEE163 pKa = 5.45LCLDD167 pKa = 4.0EE168 pKa = 5.89VSLLTGGEE176 pKa = 4.1VEE178 pKa = 4.41EE179 pKa = 4.86YY180 pKa = 10.48EE181 pKa = 4.57PEE183 pKa = 3.78TGPRR187 pKa = 11.84VRR189 pKa = 11.84VNQVGYY195 pKa = 10.72LPDD198 pKa = 3.93GPKK201 pKa = 10.39NATLVTDD208 pKa = 3.93ATSPVAWEE216 pKa = 4.49LRR218 pKa = 11.84DD219 pKa = 3.68SAGTAVASGEE229 pKa = 4.29TTPEE233 pKa = 3.89GTDD236 pKa = 3.23PSAGLNVHH244 pKa = 5.89TVDD247 pKa = 4.73FSGVDD252 pKa = 3.22ATGDD256 pKa = 3.91GFVLAADD263 pKa = 4.44GEE265 pKa = 4.31EE266 pKa = 4.44SYY268 pKa = 10.88PFEE271 pKa = 5.51ISATIYY277 pKa = 10.49DD278 pKa = 3.86QLRR281 pKa = 11.84ADD283 pKa = 3.87SLGIYY288 pKa = 7.12YY289 pKa = 7.43TQRR292 pKa = 11.84SGQEE296 pKa = 3.69IVPVEE301 pKa = 3.96VDD303 pKa = 3.53GEE305 pKa = 4.29QLKK308 pKa = 9.15TEE310 pKa = 4.2YY311 pKa = 10.55ARR313 pKa = 11.84PAGHH317 pKa = 6.2VSEE320 pKa = 5.73AGGDD324 pKa = 3.7DD325 pKa = 3.91VNQGDD330 pKa = 4.31VNVPCLPPEE339 pKa = 4.25GAVDD343 pKa = 3.54NSGNAQLGGDD353 pKa = 3.71DD354 pKa = 4.5HH355 pKa = 8.67YY356 pKa = 12.01GEE358 pKa = 5.23NGWACPEE365 pKa = 4.65GYY367 pKa = 10.61AIDD370 pKa = 5.23ASGGWYY376 pKa = 9.95DD377 pKa = 4.57AGDD380 pKa = 3.49HH381 pKa = 6.07GKK383 pKa = 10.29YY384 pKa = 9.76VVNSGISVYY393 pKa = 10.52QLLSTYY399 pKa = 9.53EE400 pKa = 3.75RR401 pKa = 11.84SLYY404 pKa = 10.83ADD406 pKa = 3.69TVSDD410 pKa = 3.99GALGDD415 pKa = 3.8STLVIPEE422 pKa = 4.3RR423 pKa = 11.84DD424 pKa = 3.3NGVPDD429 pKa = 3.43VLDD432 pKa = 3.87EE433 pKa = 4.34VRR435 pKa = 11.84HH436 pKa = 5.41NLDD439 pKa = 2.76WMLSMQVDD447 pKa = 4.32DD448 pKa = 4.59GVEE451 pKa = 3.76MTIDD455 pKa = 3.86GEE457 pKa = 4.34QYY459 pKa = 10.61DD460 pKa = 4.13AGGLAHH466 pKa = 7.08HH467 pKa = 7.05KK468 pKa = 10.21LHH470 pKa = 7.26DD471 pKa = 4.07IAWTGMNLLPHH482 pKa = 7.32EE483 pKa = 5.21DD484 pKa = 3.31PMPRR488 pKa = 11.84YY489 pKa = 8.6VHH491 pKa = 6.65RR492 pKa = 11.84PSTAATLNLAAAAAQGARR510 pKa = 11.84LYY512 pKa = 10.97ADD514 pKa = 4.3HH515 pKa = 7.26DD516 pKa = 3.54QAYY519 pKa = 10.59ADD521 pKa = 4.46EE522 pKa = 5.02LLDD525 pKa = 3.75AARR528 pKa = 11.84RR529 pKa = 11.84AWDD532 pKa = 3.5AASAHH537 pKa = 6.98PDD539 pKa = 3.32IIAPDD544 pKa = 4.07TNSLDD549 pKa = 3.93PNPGGGPYY557 pKa = 9.93NDD559 pKa = 3.9SSVGDD564 pKa = 3.69EE565 pKa = 5.01FYY567 pKa = 10.44WAAAQLFLTTGEE579 pKa = 4.38TEE581 pKa = 3.96FADD584 pKa = 3.94AVQSSPYY591 pKa = 9.81HH592 pKa = 5.95VGGAEE597 pKa = 3.87GDD599 pKa = 2.85IWRR602 pKa = 11.84ATGFDD607 pKa = 3.2WGFTAAAARR616 pKa = 11.84LDD618 pKa = 3.95LATVPNALPGRR629 pKa = 11.84DD630 pKa = 3.21AVVDD634 pKa = 4.14SVIEE638 pKa = 4.18GADD641 pKa = 3.14RR642 pKa = 11.84HH643 pKa = 5.97VATQQDD649 pKa = 4.19EE650 pKa = 4.85PFGHH654 pKa = 7.28AYY656 pKa = 10.45DD657 pKa = 4.78PGDD660 pKa = 3.73YY661 pKa = 10.59DD662 pKa = 3.02WGSNHH667 pKa = 5.91QVINNAVIIATAYY680 pKa = 10.67DD681 pKa = 3.75LTGEE685 pKa = 4.03AQYY688 pKa = 11.29RR689 pKa = 11.84DD690 pKa = 3.52AALEE694 pKa = 4.02TFDD697 pKa = 4.72YY698 pKa = 11.2VLGRR702 pKa = 11.84NALNNSYY709 pKa = 10.96VKK711 pKa = 10.79GYY713 pKa = 7.92GTQFSQNMHH722 pKa = 5.83NRR724 pKa = 11.84WMASADD730 pKa = 4.05RR731 pKa = 11.84LPPHH735 pKa = 7.4PDD737 pKa = 2.59GMLAAGPNSAIQDD750 pKa = 4.15PIAQEE755 pKa = 4.1KK756 pKa = 10.07LQGCAPQFCYY766 pKa = 9.79IDD768 pKa = 4.97EE769 pKa = 4.31VDD771 pKa = 3.25SWSTNEE777 pKa = 4.19MTINWNAPLSWFASWADD794 pKa = 3.59DD795 pKa = 3.58VANAGGDD802 pKa = 3.95VIADD806 pKa = 3.46ACTAEE811 pKa = 4.46LTVHH815 pKa = 6.47SDD817 pKa = 2.69WGSGFHH823 pKa = 5.62ATVRR827 pKa = 11.84VTAGDD832 pKa = 3.82APLSGWTTSFDD843 pKa = 3.55LGQATLANGWNAEE856 pKa = 3.93FSTSGSQVTASDD868 pKa = 3.82AGWNGTMAEE877 pKa = 4.26GGSTEE882 pKa = 4.02FGFVGSGAPPSSIPVACSAGG902 pKa = 3.39

Molecular weight: 95.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N4Z434|A0A3N4Z434_9MICO Uncharacterized protein OS=Myceligenerans xiligouense OX=253184 GN=EDD34_0524 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84TILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.45GRR40 pKa = 11.84AKK42 pKa = 10.69LSAA45 pKa = 3.92

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3975

0

3975

1408027

29

5751

354.2

37.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.769 ± 0.065

0.586 ± 0.009

6.649 ± 0.032

5.781 ± 0.038

2.643 ± 0.021

9.701 ± 0.036

2.147 ± 0.018

3.242 ± 0.026

1.649 ± 0.027

9.726 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.727 ± 0.016

1.757 ± 0.021

5.98 ± 0.034

2.545 ± 0.018

7.798 ± 0.047

5.133 ± 0.029

6.409 ± 0.032

9.233 ± 0.039

1.591 ± 0.019

1.932 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski