Microbacterium phage Nucci

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Elerivirus; unclassified Elerivirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514U0J1|A0A514U0J1_9CAUD Thymidylate synthase OS=Microbacterium phage Nucci OX=2593340 GN=48 PE=3 SV=1
MM1 pKa = 7.89GYY3 pKa = 10.25LCDD6 pKa = 3.85HH7 pKa = 7.34CDD9 pKa = 3.01VSLQVDD15 pKa = 3.63PNGYY19 pKa = 9.66FVDD22 pKa = 4.46DD23 pKa = 4.93SGSSDD28 pKa = 3.62CPKK31 pKa = 10.93DD32 pKa = 3.67EE33 pKa = 5.78GGHH36 pKa = 4.3TWEE39 pKa = 4.99GRR41 pKa = 11.84AGLL44 pKa = 3.97

Molecular weight: 4.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514U0G5|A0A514U0G5_9CAUD Thymidylate kinase OS=Microbacterium phage Nucci OX=2593340 GN=44 PE=4 SV=1
MM1 pKa = 8.0DD2 pKa = 6.06PDD4 pKa = 3.73KK5 pKa = 11.44VLATVSPALRR15 pKa = 11.84ASLEE19 pKa = 3.99RR20 pKa = 11.84QLIQINRR27 pKa = 11.84NYY29 pKa = 9.22MAALARR35 pKa = 11.84LYY37 pKa = 10.4QVDD40 pKa = 3.41IRR42 pKa = 11.84TVLIWHH48 pKa = 5.95ATGRR52 pKa = 11.84VV53 pKa = 3.21

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12789

35

808

203.0

22.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.048 ± 0.431

0.672 ± 0.096

6.662 ± 0.306

5.958 ± 0.42

3.049 ± 0.181

7.976 ± 0.357

1.798 ± 0.169

4.926 ± 0.384

4.676 ± 0.355

8.351 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.141

3.37 ± 0.218

4.871 ± 0.218

3.972 ± 0.2

5.88 ± 0.365

5.692 ± 0.288

6.85 ± 0.337

7.694 ± 0.276

2.096 ± 0.248

2.854 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski