Lye Green virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140HEQ6|A0A140HEQ6_9RHAB VP2 OS=Lye Green virus OX=1807808 PE=4 SV=1
MM1 pKa = 7.18YY2 pKa = 8.91PNHH5 pKa = 6.65HH6 pKa = 6.48NLRR9 pKa = 11.84GSGQHH14 pKa = 7.32DD15 pKa = 4.47DD16 pKa = 3.5IQFCEE21 pKa = 4.56GNAEE25 pKa = 4.01VLNYY29 pKa = 10.03LKK31 pKa = 10.81NVTPDD36 pKa = 3.34MDD38 pKa = 4.76NDD40 pKa = 4.13TPPLDD45 pKa = 3.4WTEE48 pKa = 3.88EE49 pKa = 4.35TFPSMAMGSKK59 pKa = 9.84IKK61 pKa = 9.47TRR63 pKa = 11.84PSILDD68 pKa = 3.29QVNAASIVLNWYY80 pKa = 9.55VSGIQRR86 pKa = 11.84RR87 pKa = 11.84ILVPIEE93 pKa = 3.84TVIVGMTTSGDD104 pKa = 3.34ANYY107 pKa = 10.33VFGDD111 pKa = 4.07EE112 pKa = 5.05IFDD115 pKa = 3.52ATTGIEE121 pKa = 4.68LEE123 pKa = 4.13PQPAVEE129 pKa = 4.44MLTPSEE135 pKa = 4.16LEE137 pKa = 3.59RR138 pKa = 11.84WRR140 pKa = 11.84AAAIGAGPEE149 pKa = 4.17LPPMNPMGGIPIAQGDD165 pKa = 3.9ASKK168 pKa = 10.55LASAVPDD175 pKa = 3.35TTLWYY180 pKa = 10.77KK181 pKa = 11.02KK182 pKa = 8.05MWEE185 pKa = 4.11LLHH188 pKa = 5.96VVVVADD194 pKa = 3.55SAEE197 pKa = 4.32TAEE200 pKa = 4.14QTAGRR205 pKa = 11.84RR206 pKa = 11.84YY207 pKa = 8.21ALNYY211 pKa = 9.25IGYY214 pKa = 8.69ICLLVIRR221 pKa = 11.84GVAKK225 pKa = 10.58NHH227 pKa = 5.61ATVIAALEE235 pKa = 4.17KK236 pKa = 10.5NLPDD240 pKa = 3.35RR241 pKa = 11.84VTAFWPYY248 pKa = 10.73DD249 pKa = 3.24HH250 pKa = 7.19TMPKK254 pKa = 10.29PPMGHH259 pKa = 7.27DD260 pKa = 2.9ISIRR264 pKa = 11.84DD265 pKa = 3.43IEE267 pKa = 4.31NALEE271 pKa = 4.3RR272 pKa = 11.84GSSHH276 pKa = 5.57TRR278 pKa = 11.84RR279 pKa = 11.84LLVNLIQSSLTTEE292 pKa = 3.74PTKK295 pKa = 10.71RR296 pKa = 11.84GILMSGCMKK305 pKa = 10.53SLQDD309 pKa = 3.45SGLGALHH316 pKa = 6.45WVFEE320 pKa = 4.22ASRR323 pKa = 11.84AIEE326 pKa = 4.07VEE328 pKa = 3.77PRR330 pKa = 11.84ALMEE334 pKa = 4.22AMFYY338 pKa = 11.16GPFIGSTMVVGRR350 pKa = 11.84FLSEE354 pKa = 3.98AKK356 pKa = 10.33NSSRR360 pKa = 11.84SWKK363 pKa = 7.89WARR366 pKa = 11.84VLNDD370 pKa = 3.94GYY372 pKa = 10.14LTALSVKK379 pKa = 10.06NNKK382 pKa = 9.53IYY384 pKa = 10.92VATLVALVAEE394 pKa = 5.3LKK396 pKa = 10.02PDD398 pKa = 3.52HH399 pKa = 7.05AIWNIPSLALSNWMKK414 pKa = 10.6HH415 pKa = 5.05RR416 pKa = 11.84ALEE419 pKa = 4.21HH420 pKa = 5.86AQSISAEE427 pKa = 4.3LYY429 pKa = 10.29DD430 pKa = 4.34AAHH433 pKa = 6.32FQAGSTSGKK442 pKa = 10.02NVIARR447 pKa = 11.84IRR449 pKa = 11.84LAKK452 pKa = 10.5ANARR456 pKa = 11.84LTTIPEE462 pKa = 4.38ANPLPNVQAQMMDD475 pKa = 3.48DD476 pKa = 3.65QEE478 pKa = 5.06LGEE481 pKa = 4.49VII483 pKa = 4.57

Molecular weight: 53.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140HEQ8|A0A140HEQ8_9RHAB Putative glycoprotein OS=Lye Green virus OX=1807808 PE=4 SV=1
MM1 pKa = 7.4NLLPGASIHH10 pKa = 6.02QRR12 pKa = 11.84DD13 pKa = 3.9KK14 pKa = 10.75ISRR17 pKa = 11.84FRR19 pKa = 11.84KK20 pKa = 9.44NVLHH24 pKa = 6.79LEE26 pKa = 4.16KK27 pKa = 10.72VVNKK31 pKa = 9.75VFTSYY36 pKa = 10.72TITFVKK42 pKa = 8.78PTSNMSSRR50 pKa = 11.84LIPRR54 pKa = 11.84IPVTKK59 pKa = 10.46SPLKK63 pKa = 9.63TAIQVEE69 pKa = 4.23LRR71 pKa = 11.84LWGAQGLDD79 pKa = 3.07NTLLKK84 pKa = 10.14KK85 pKa = 10.36ICVCVIWEE93 pKa = 4.31SYY95 pKa = 10.18KK96 pKa = 11.15GVVDD100 pKa = 5.86DD101 pKa = 4.85PEE103 pKa = 4.44LWSHH107 pKa = 7.05ASQALYY113 pKa = 10.8QRR115 pKa = 11.84MKK117 pKa = 10.47RR118 pKa = 11.84KK119 pKa = 10.26CEE121 pKa = 3.55WAPIMSQSSAQFLVSTTWFSLHH143 pKa = 6.47PRR145 pKa = 11.84NLYY148 pKa = 10.43EE149 pKa = 4.5GFSQKK154 pKa = 8.89RR155 pKa = 11.84TFSRR159 pKa = 11.84LFHH162 pKa = 6.01FHH164 pKa = 6.88SPTGGILSVDD174 pKa = 3.38YY175 pKa = 11.25VITLRR180 pKa = 11.84HH181 pKa = 5.25NTWDD185 pKa = 3.3LKK187 pKa = 10.94EE188 pKa = 4.05YY189 pKa = 11.1GNMVLMGYY197 pKa = 10.2LCLEE201 pKa = 4.02QTNYY205 pKa = 9.87VPNPKK210 pKa = 10.2GVISLAIEE218 pKa = 4.16DD219 pKa = 4.84LKK221 pKa = 11.23PLINFDD227 pKa = 3.83PYY229 pKa = 11.62QMVRR233 pKa = 11.84LLGLSPMIEE242 pKa = 4.4DD243 pKa = 6.03PIPAPSKK250 pKa = 9.58TRR252 pKa = 11.84SILPAKK258 pKa = 9.99FFKK261 pKa = 10.75KK262 pKa = 10.49

Molecular weight: 30.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

4163

262

2328

832.6

86.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.02 ± 1.042

1.417 ± 0.21

4.228 ± 0.32

4.66 ± 0.271

3.579 ± 0.393

4.588 ± 0.414

2.618 ± 0.137

6.101 ± 0.378

4.3 ± 0.751

10.545 ± 0.427

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.321

4.396 ± 0.261

5.213 ± 0.498

3.843 ± 0.364

5.237 ± 0.064

7.278 ± 0.848

5.525 ± 0.599

4.66 ± 0.578

1.513 ± 0.254

3.603 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski