BtMf-AlphaCoV/HeN2013

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Minunacovirus; Miniopterus bat coronavirus HKU8

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1WHE0|A0A0U1WHE0_9ALPC Envelope small membrane protein OS=BtMf-AlphaCoV/HeN2013 OX=1503283 GN=E PE=3 SV=1
MM1 pKa = 7.64LLLLFITAVSAANNNCVGNGDD22 pKa = 4.5LDD24 pKa = 4.11SMAKK28 pKa = 10.14LKK30 pKa = 10.94LGLPPNITNAYY41 pKa = 7.98VSGYY45 pKa = 10.68LPLPGNWVCKK55 pKa = 10.28AVGNVVNAHH64 pKa = 4.83VHH66 pKa = 5.74RR67 pKa = 11.84NAHH70 pKa = 5.32GVFMSYY76 pKa = 10.96YY77 pKa = 10.75SDD79 pKa = 3.68AQDD82 pKa = 3.29VAIGVGTSKK91 pKa = 11.22SNNSWGMYY99 pKa = 8.98FFQKK103 pKa = 10.01NSEE106 pKa = 3.69YY107 pKa = 10.25RR108 pKa = 11.84AIVRR112 pKa = 11.84ICRR115 pKa = 11.84WSAYY119 pKa = 9.3KK120 pKa = 10.61LPNYY124 pKa = 9.32GPNPADD130 pKa = 3.57SRR132 pKa = 11.84QEE134 pKa = 3.95CLFNKK139 pKa = 8.87QVPLTFLHH147 pKa = 6.07QGNEE151 pKa = 3.99IVGATWSGDD160 pKa = 3.18KK161 pKa = 10.78VVLYY165 pKa = 9.96TRR167 pKa = 11.84TGLSTFYY174 pKa = 11.26VPGAAEE180 pKa = 3.86WDD182 pKa = 4.01VVSIRR187 pKa = 11.84CKK189 pKa = 9.95SPKK192 pKa = 9.52VCAHH196 pKa = 4.66QVVVNASTVVVSTGANGLITNYY218 pKa = 10.05SICKK222 pKa = 8.65NCSGFPDD229 pKa = 3.43HH230 pKa = 7.06VFAAGQDD237 pKa = 3.81GTIPPSFGFEE247 pKa = 3.19NWFYY251 pKa = 10.23LTNSSSPVSGRR262 pKa = 11.84FSSSQPLQLFCLWPIPALLGNTLPVYY288 pKa = 10.95FNMSKK293 pKa = 10.99NNANCNGFKK302 pKa = 10.56ADD304 pKa = 3.76GVVDD308 pKa = 3.76SLRR311 pKa = 11.84FALNFSDD318 pKa = 3.24EE319 pKa = 4.13RR320 pKa = 11.84AFARR324 pKa = 11.84EE325 pKa = 4.09GVISLEE331 pKa = 4.52TVGNTYY337 pKa = 11.14NFTCTNSSTPTDD349 pKa = 3.13GSTFVLPFGVTDD361 pKa = 3.3QAYY364 pKa = 8.11YY365 pKa = 11.27CFMTFYY371 pKa = 11.26LNRR374 pKa = 11.84TADD377 pKa = 3.22LRR379 pKa = 11.84TTVFAGMLPPVVRR392 pKa = 11.84EE393 pKa = 3.72FVVMRR398 pKa = 11.84NGDD401 pKa = 3.5FYY403 pKa = 11.84LNGFRR408 pKa = 11.84VFSVGQVVSARR419 pKa = 11.84FNISSNDD426 pKa = 3.22SRR428 pKa = 11.84DD429 pKa = 3.15FWTVAFANNAPVLVDD444 pKa = 3.49INSTNIQRR452 pKa = 11.84LLYY455 pKa = 10.49CNSPLNIIKK464 pKa = 9.81CQQLRR469 pKa = 11.84FNLDD473 pKa = 2.99NGFYY477 pKa = 10.41SYY479 pKa = 11.56APEE482 pKa = 4.08TANNLPRR489 pKa = 11.84SFVRR493 pKa = 11.84LPKK496 pKa = 10.78YY497 pKa = 7.14MTHH500 pKa = 5.98SVINVSVAVSYY511 pKa = 11.2DD512 pKa = 3.45VVNKK516 pKa = 9.7TNIGLHH522 pKa = 5.15AWRR525 pKa = 11.84VSFADD530 pKa = 3.2RR531 pKa = 11.84WLDD534 pKa = 3.38YY535 pKa = 11.55DD536 pKa = 3.8KK537 pKa = 11.52DD538 pKa = 3.65NRR540 pKa = 11.84TAVCADD546 pKa = 3.27SASFTMRR553 pKa = 11.84LIKK556 pKa = 10.66GSFSYY561 pKa = 11.42GDD563 pKa = 3.5VYY565 pKa = 11.51VDD567 pKa = 3.26IWSGEE572 pKa = 4.31CPFSFAALNNYY583 pKa = 10.0LSFGSICFSLVPNGGCAMSIVTRR606 pKa = 11.84GSYY609 pKa = 10.82GEE611 pKa = 4.03PQKK614 pKa = 10.78FAALYY619 pKa = 9.61VSFSEE624 pKa = 4.16GDD626 pKa = 3.54NVIGVPKK633 pKa = 10.54APLPGVLDD641 pKa = 3.75MSEE644 pKa = 3.7VHH646 pKa = 6.36YY647 pKa = 10.24NVCTSYY653 pKa = 10.6TIYY656 pKa = 10.56GHH658 pKa = 5.65TGRR661 pKa = 11.84GVISRR666 pKa = 11.84GPVDD670 pKa = 3.98YY671 pKa = 11.36VSGLYY676 pKa = 8.11YY677 pKa = 10.34TSPAGDD683 pKa = 3.43LLAYY687 pKa = 9.79KK688 pKa = 10.47NSTTGEE694 pKa = 3.91VFMIQPCQFASQVAVVSDD712 pKa = 4.83AIVGVASSSATSSIPFNDD730 pKa = 3.37TVDD733 pKa = 3.11AGRR736 pKa = 11.84FYY738 pKa = 11.45YY739 pKa = 10.49HH740 pKa = 6.89FNNVTEE746 pKa = 4.37VNGTKK751 pKa = 10.51CEE753 pKa = 4.15VPTLTYY759 pKa = 10.66GGLGICADD767 pKa = 3.9GKK769 pKa = 10.41IVNATRR775 pKa = 11.84RR776 pKa = 11.84YY777 pKa = 8.93AAPEE781 pKa = 4.58PISPVVTGNISVPMNFSFSVQVEE804 pKa = 4.35YY805 pKa = 10.52IQIMLKK811 pKa = 10.27PVTVDD816 pKa = 2.84CSVYY820 pKa = 10.32VCNGNPRR827 pKa = 11.84CLQLLSQYY835 pKa = 11.5ASACKK840 pKa = 9.77TIEE843 pKa = 3.88QALQLSARR851 pKa = 11.84LEE853 pKa = 4.32SVEE856 pKa = 4.18VNSMVAISDD865 pKa = 3.53TALNLGVISNFDD877 pKa = 3.29NTFNLSNVLPASVGGKK893 pKa = 8.93SVIEE897 pKa = 4.3DD898 pKa = 3.72LLFDD902 pKa = 3.94KK903 pKa = 11.1VVTSGLGTVDD913 pKa = 3.3TDD915 pKa = 3.77YY916 pKa = 11.21KK917 pKa = 11.24ACAEE921 pKa = 4.05KK922 pKa = 9.97MANTIAEE929 pKa = 4.49AGCVQYY935 pKa = 11.5YY936 pKa = 9.78NGIMVLPGVVDD947 pKa = 3.64QSLLGQYY954 pKa = 7.73TAALTGAMVMGGITAAAAIPFSIAVQSRR982 pKa = 11.84LNYY985 pKa = 10.06LALQTDD991 pKa = 4.07VLQRR995 pKa = 11.84NQQILAQSFNSAMGNITNAFEE1016 pKa = 4.4NVGNAIEE1023 pKa = 4.12QTSQGLQTVAQALDD1037 pKa = 3.47KK1038 pKa = 11.0VQNAVNEE1045 pKa = 4.12QGNALSHH1052 pKa = 5.84LTKK1055 pKa = 10.55QLASNFQAISASIEE1069 pKa = 3.88DD1070 pKa = 4.01LYY1072 pKa = 11.8VRR1074 pKa = 11.84LGQVEE1079 pKa = 4.59ADD1081 pKa = 3.54QQVDD1085 pKa = 3.49RR1086 pKa = 11.84LITGRR1091 pKa = 11.84LAALNAFVSQQLTKK1105 pKa = 9.57YY1106 pKa = 8.06TDD1108 pKa = 3.16VRR1110 pKa = 11.84ASRR1113 pKa = 11.84QLAQEE1118 pKa = 4.75KK1119 pKa = 10.45INEE1122 pKa = 4.44CVKK1125 pKa = 10.63SQSFRR1130 pKa = 11.84YY1131 pKa = 8.66GFCGNGTHH1139 pKa = 5.87VFSVANAAPDD1149 pKa = 4.35GIMFFHH1155 pKa = 7.06SVLLPTAYY1163 pKa = 10.48AEE1165 pKa = 4.25VNAYY1169 pKa = 10.26AGLCVDD1175 pKa = 3.93DD1176 pKa = 4.38KK1177 pKa = 11.57GYY1179 pKa = 11.11VLRR1182 pKa = 11.84DD1183 pKa = 3.4PGNVLFQKK1191 pKa = 10.22PGNDD1195 pKa = 3.33TYY1197 pKa = 11.46LITPRR1202 pKa = 11.84RR1203 pKa = 11.84MFEE1206 pKa = 3.55PRR1208 pKa = 11.84IPQMSDD1214 pKa = 2.85FVQISGCNVSYY1225 pKa = 11.58VNVSSEE1231 pKa = 4.15NLPDD1235 pKa = 3.62IVPDD1239 pKa = 4.34FMDD1242 pKa = 3.64VNATIQDD1249 pKa = 3.82ILDD1252 pKa = 3.66KK1253 pKa = 10.5LQNYY1257 pKa = 7.67SKK1259 pKa = 10.9PDD1261 pKa = 3.42FDD1263 pKa = 6.28LDD1265 pKa = 3.78IFNVTYY1271 pKa = 10.93LNLSAEE1277 pKa = 4.48INDD1280 pKa = 3.59LTNRR1284 pKa = 11.84SQQLQAITDD1293 pKa = 3.75EE1294 pKa = 4.24LRR1296 pKa = 11.84ATIANINGTLVDD1308 pKa = 4.67LEE1310 pKa = 4.09WLNRR1314 pKa = 11.84VEE1316 pKa = 5.97TYY1318 pKa = 10.4IKK1320 pKa = 9.3WPWYY1324 pKa = 7.96VWLAIAVALIVLTGLMLWCCLSTGCCGCCSCMASTLDD1361 pKa = 3.63FRR1363 pKa = 11.84GSRR1366 pKa = 11.84LQQYY1370 pKa = 7.6EE1371 pKa = 4.15VEE1373 pKa = 4.46KK1374 pKa = 11.13VHH1376 pKa = 6.56IQQ1378 pKa = 3.09

Molecular weight: 150.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1WHD9|A0A0U1WHD9_9ALPC Spike glycoprotein OS=BtMf-AlphaCoV/HeN2013 OX=1503283 PE=4 SV=1
MM1 pKa = 7.58FLRR4 pKa = 11.84TIRR7 pKa = 11.84TSLSSSNRR15 pKa = 11.84LMLIWTLLARR25 pKa = 11.84TNQRR29 pKa = 11.84SSANLRR35 pKa = 11.84KK36 pKa = 9.47RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.19RR41 pKa = 11.84HH42 pKa = 5.65PLNSTLQHH50 pKa = 6.16LCSHH54 pKa = 7.28LLLCCQTLCLKK65 pKa = 10.95LSLRR69 pKa = 11.84WLTRR73 pKa = 11.84LLMLSLSLLHH83 pKa = 6.53EE84 pKa = 5.16LSTKK88 pKa = 10.7LIMLAFVVFLAAIVSSLSIPVRR110 pKa = 11.84HH111 pKa = 6.55NYY113 pKa = 10.18LDD115 pKa = 3.99PNGPNGFRR123 pKa = 11.84DD124 pKa = 4.09GLLLQCRR131 pKa = 11.84HH132 pKa = 5.38SLVWFDD138 pKa = 4.35FSEE141 pKa = 4.15LAIPLACMRR150 pKa = 11.84YY151 pKa = 8.0MQLLQPDD158 pKa = 4.79LGTLFKK164 pKa = 11.01PEE166 pKa = 3.64VRR168 pKa = 11.84VTHH171 pKa = 6.67FDD173 pKa = 3.28AAVQRR178 pKa = 11.84HH179 pKa = 6.05RR180 pKa = 11.84IVVDD184 pKa = 3.28KK185 pKa = 11.15LEE187 pKa = 4.32RR188 pKa = 11.84AADD191 pKa = 3.99ADD193 pKa = 4.14WLHH196 pKa = 5.71VAYY199 pKa = 9.36TRR201 pKa = 11.84LSGFHH206 pKa = 6.45EE207 pKa = 4.72EE208 pKa = 4.3IIRR211 pKa = 11.84LGGDD215 pKa = 3.05SFAARR220 pKa = 11.84DD221 pKa = 3.75ALEE224 pKa = 4.41KK225 pKa = 10.26KK226 pKa = 9.31YY227 pKa = 10.72HH228 pKa = 6.62PSTTGSTKK236 pKa = 9.92IRR238 pKa = 11.84GG239 pKa = 3.51

Molecular weight: 27.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

9476

74

6889

1353.7

149.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.303 ± 0.362

3.345 ± 0.592

5.424 ± 0.6

3.862 ± 0.418

5.804 ± 0.359

6.859 ± 0.423

1.794 ± 0.269

4.485 ± 0.613

5.245 ± 0.979

8.643 ± 1.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.072

5.498 ± 0.598

3.957 ± 0.686

2.955 ± 0.735

3.81 ± 0.623

7.007 ± 0.722

6.11 ± 0.301

10.31 ± 0.81

1.203 ± 0.222

4.401 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski