Melandrium yellow fleck virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Bromovirus

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C8KH77|C8KH77_9BROM Movement protein OS=Melandrium yellow fleck virus OX=545259 GN=3a PE=4 SV=1
MM1 pKa = 7.45AFEE4 pKa = 4.71IEE6 pKa = 4.35YY7 pKa = 9.88EE8 pKa = 4.39VPSFQWMLDD17 pKa = 3.17TSRR20 pKa = 11.84LDD22 pKa = 3.53NYY24 pKa = 10.83SLNNGEE30 pKa = 4.52PSNIIPVDD38 pKa = 3.71DD39 pKa = 4.85SIEE42 pKa = 4.03IVADD46 pKa = 3.91GTLSSYY52 pKa = 10.96LHH54 pKa = 6.13AVKK57 pKa = 9.92PLEE60 pKa = 4.22IGGSYY65 pKa = 10.0EE66 pKa = 3.95PPFEE70 pKa = 3.94QSRR73 pKa = 11.84WAQCCEE79 pKa = 3.44NAINVSKK86 pKa = 10.48MKK88 pKa = 10.73GVILKK93 pKa = 8.24PLPEE97 pKa = 4.15VARR100 pKa = 11.84ILYY103 pKa = 10.17LDD105 pKa = 4.18LDD107 pKa = 4.12EE108 pKa = 6.02PIVDD112 pKa = 3.86EE113 pKa = 5.59SEE115 pKa = 3.97VDD117 pKa = 3.09DD118 pKa = 4.35WRR120 pKa = 11.84PIDD123 pKa = 3.74TSDD126 pKa = 3.85GYY128 pKa = 11.32DD129 pKa = 3.76PEE131 pKa = 4.46TSDD134 pKa = 4.45WDD136 pKa = 3.51PTYY139 pKa = 11.29LDD141 pKa = 4.07RR142 pKa = 11.84EE143 pKa = 4.35ADD145 pKa = 3.35AKK147 pKa = 11.11AYY149 pKa = 10.21GHH151 pKa = 7.39LLPSYY156 pKa = 6.88QQKK159 pKa = 10.36EE160 pKa = 4.02IAVSDD165 pKa = 4.09VEE167 pKa = 4.48TDD169 pKa = 3.43RR170 pKa = 11.84QNQDD174 pKa = 2.7VKK176 pKa = 11.37NVLLNPDD183 pKa = 4.34PLPARR188 pKa = 11.84EE189 pKa = 4.03FTLGDD194 pKa = 2.71RR195 pKa = 11.84FMPIEE200 pKa = 4.2EE201 pKa = 4.33EE202 pKa = 4.16FGGTGADD209 pKa = 3.26YY210 pKa = 11.18DD211 pKa = 3.98VTLNLINPVEE221 pKa = 4.27SRR223 pKa = 11.84VARR226 pKa = 11.84VVDD229 pKa = 3.5TLGGLEE235 pKa = 4.18SGVTLTSPLFLEE247 pKa = 4.32RR248 pKa = 11.84VSLNRR253 pKa = 11.84VDD255 pKa = 5.05AVGHH259 pKa = 6.08HH260 pKa = 6.44MLPTHH265 pKa = 7.31AYY267 pKa = 10.21FDD269 pKa = 3.68DD270 pKa = 4.06TYY272 pKa = 11.36YY273 pKa = 10.79QALEE277 pKa = 4.13EE278 pKa = 4.21NADD281 pKa = 3.57YY282 pKa = 11.36SSDD285 pKa = 3.67FNKK288 pKa = 10.64LKK290 pKa = 10.45IKK292 pKa = 10.64QSDD295 pKa = 3.67VDD297 pKa = 3.6WYY299 pKa = 11.05RR300 pKa = 11.84DD301 pKa = 3.19PDD303 pKa = 3.73KK304 pKa = 11.26YY305 pKa = 11.27YY306 pKa = 10.84EE307 pKa = 4.43PNLTIGSFQKK317 pKa = 10.71RR318 pKa = 11.84IGTQKK323 pKa = 9.58TVLTALKK330 pKa = 9.92KK331 pKa = 10.5RR332 pKa = 11.84NADD335 pKa = 3.46VPEE338 pKa = 4.18MSDD341 pKa = 3.53AIDD344 pKa = 3.48VKK346 pKa = 10.68RR347 pKa = 11.84VARR350 pKa = 11.84EE351 pKa = 3.74VADD354 pKa = 3.98KK355 pKa = 10.91FISSYY360 pKa = 11.46LNVDD364 pKa = 3.6GKK366 pKa = 10.92DD367 pKa = 3.51CFINGLNVMAKK378 pKa = 10.47GLDD381 pKa = 3.9YY382 pKa = 10.6YY383 pKa = 11.2KK384 pKa = 10.33KK385 pKa = 9.38WKK387 pKa = 9.68DD388 pKa = 3.19HH389 pKa = 6.82RR390 pKa = 11.84EE391 pKa = 3.95LSGVTIATGEE401 pKa = 3.85NLQRR405 pKa = 11.84YY406 pKa = 6.22QHH408 pKa = 6.22MIKK411 pKa = 9.56TDD413 pKa = 3.31IKK415 pKa = 10.77PVVTDD420 pKa = 3.53TLHH423 pKa = 7.11LDD425 pKa = 3.35RR426 pKa = 11.84AIAATITFHH435 pKa = 6.66GKK437 pKa = 10.12GVTSCFSPFFTACFEE452 pKa = 4.22NFSMALKK459 pKa = 10.51SRR461 pKa = 11.84FVVPIGKK468 pKa = 9.37ISSLEE473 pKa = 3.86IPNVSLNNKK482 pKa = 8.32WFLEE486 pKa = 4.07ADD488 pKa = 3.42LSKK491 pKa = 10.63FDD493 pKa = 3.7KK494 pKa = 11.18SQGEE498 pKa = 3.98LHH500 pKa = 7.06LEE502 pKa = 4.06FQRR505 pKa = 11.84EE506 pKa = 3.85ILHH509 pKa = 6.78AIGFPVHH516 pKa = 6.79LSNWWADD523 pKa = 4.13FHH525 pKa = 7.55RR526 pKa = 11.84EE527 pKa = 4.21SYY529 pKa = 11.39LNDD532 pKa = 3.54PNAKK536 pKa = 9.64VSLPVSFQRR545 pKa = 11.84RR546 pKa = 11.84TGDD549 pKa = 2.78AFTYY553 pKa = 10.13FGNTIVTMAMMAYY566 pKa = 8.3TFDD569 pKa = 3.62MNLPEE574 pKa = 4.27LAIFSGDD581 pKa = 3.91DD582 pKa = 3.31SLLVLNDD589 pKa = 3.85KK590 pKa = 10.79PSIDD594 pKa = 3.27TDD596 pKa = 3.19IFQRR600 pKa = 11.84LFNMEE605 pKa = 4.22VKK607 pKa = 10.26IMEE610 pKa = 4.38PSVPYY615 pKa = 10.34VCSKK619 pKa = 10.75FLLEE623 pKa = 4.32TEE625 pKa = 4.41LGSVVSVPDD634 pKa = 4.06PLRR637 pKa = 11.84EE638 pKa = 3.71IQRR641 pKa = 11.84MSKK644 pKa = 10.36RR645 pKa = 11.84KK646 pKa = 8.98ILKK649 pKa = 10.14DD650 pKa = 3.49PEE652 pKa = 3.78VLRR655 pKa = 11.84AHH657 pKa = 6.16FTSFKK662 pKa = 10.89DD663 pKa = 3.25RR664 pKa = 11.84MRR666 pKa = 11.84FLDD669 pKa = 3.56VLDD672 pKa = 4.82DD673 pKa = 4.07RR674 pKa = 11.84MISILCRR681 pKa = 11.84YY682 pKa = 9.56VSLKK686 pKa = 9.64YY687 pKa = 10.43LKK689 pKa = 10.18PGLEE693 pKa = 3.72QDD695 pKa = 3.3VRR697 pKa = 11.84EE698 pKa = 4.21ALACFSYY705 pKa = 10.75YY706 pKa = 10.25SEE708 pKa = 4.31SFFRR712 pKa = 11.84FAEE715 pKa = 5.05LYY717 pKa = 9.82AQDD720 pKa = 3.87GKK722 pKa = 10.95FVYY725 pKa = 9.54QVKK728 pKa = 10.62DD729 pKa = 3.92PISHH733 pKa = 6.4HH734 pKa = 6.7RR735 pKa = 11.84GDD737 pKa = 4.53EE738 pKa = 3.99ILGSRR743 pKa = 11.84EE744 pKa = 3.38KK745 pKa = 11.16DD746 pKa = 2.6GDD748 pKa = 4.06YY749 pKa = 10.72FHH751 pKa = 7.06NWHH754 pKa = 6.49NPVFPRR760 pKa = 11.84ILDD763 pKa = 3.37KK764 pKa = 10.96AVRR767 pKa = 11.84VFGKK771 pKa = 10.68YY772 pKa = 8.11STDD775 pKa = 3.4YY776 pKa = 11.26SSEE779 pKa = 4.06NYY781 pKa = 9.16KK782 pKa = 10.31KK783 pKa = 10.79KK784 pKa = 10.84SMKK787 pKa = 10.5CEE789 pKa = 3.6EE790 pKa = 4.52DD791 pKa = 3.22KK792 pKa = 11.31LFRR795 pKa = 11.84KK796 pKa = 9.73SLSLAYY802 pKa = 9.87DD803 pKa = 3.24QRR805 pKa = 11.84DD806 pKa = 3.43LLKK809 pKa = 11.06NKK811 pKa = 9.74LKK813 pKa = 10.36HH814 pKa = 7.08DD815 pKa = 4.08RR816 pKa = 11.84LLDD819 pKa = 3.62EE820 pKa = 4.64TASSSFSKK828 pKa = 10.95

Molecular weight: 94.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8KH78|C8KH78_9BROM Coat protein OS=Melandrium yellow fleck virus OX=545259 GN=CP PE=4 SV=1
MM1 pKa = 7.12STPGTVKK8 pKa = 8.18MTRR11 pKa = 11.84AQRR14 pKa = 11.84RR15 pKa = 11.84AAARR19 pKa = 11.84RR20 pKa = 11.84AAKK23 pKa = 9.28PQINKK28 pKa = 9.85RR29 pKa = 11.84IQPVIVEE36 pKa = 4.61PPTSGLGRR44 pKa = 11.84AIKK47 pKa = 10.49ALGGHH52 pKa = 7.13TITKK56 pKa = 9.53WEE58 pKa = 4.33TSCPEE63 pKa = 3.64IKK65 pKa = 10.31AKK67 pKa = 10.64SSIQITIPLPSEE79 pKa = 4.05LSSEE83 pKa = 4.13RR84 pKa = 11.84NKK86 pKa = 9.91VLKK89 pKa = 10.31VGRR92 pKa = 11.84VLLWLGLLPSINGQVKK108 pKa = 10.43ACVTQTNPVPGAAFQSALAIADD130 pKa = 3.47SSKK133 pKa = 10.73EE134 pKa = 4.18VVSAMYY140 pKa = 10.85VDD142 pKa = 3.94IFKK145 pKa = 11.19GITIEE150 pKa = 5.52DD151 pKa = 4.12FVNDD155 pKa = 3.7LAIYY159 pKa = 9.59VYY161 pKa = 10.74SAVDD165 pKa = 3.36VAAKK169 pKa = 10.3DD170 pKa = 3.64IIVHH174 pKa = 6.09LEE176 pKa = 3.96VEE178 pKa = 4.46HH179 pKa = 5.9VRR181 pKa = 11.84PVLDD185 pKa = 5.11DD186 pKa = 3.74YY187 pKa = 9.14FTPLHH192 pKa = 6.2

Molecular weight: 20.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2263

192

948

565.8

63.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.275 ± 0.702

2.121 ± 0.694

7.114 ± 1.188

6.142 ± 0.552

4.242 ± 0.692

5.259 ± 0.425

2.342 ± 0.352

5.259 ± 0.691

6.894 ± 0.125

8.484 ± 0.545

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.121

4.596 ± 0.507

4.11 ± 0.885

2.872 ± 0.165

5.435 ± 0.566

8.175 ± 1.148

5.038 ± 0.4

8.263 ± 0.685

1.326 ± 0.221

3.756 ± 0.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski