Bacillus phage Eldridge

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Eldridgevirus; Bacillus virus Eldridge

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 231 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Y0DBF5|A0A0Y0DBF5_9CAUD Tail spike protein OS=Bacillus phage Eldridge OX=1776293 GN=Eldridge_087 PE=4 SV=1
MM1 pKa = 6.37TTYY4 pKa = 10.19IANVNVEE11 pKa = 4.34KK12 pKa = 10.38MVEE15 pKa = 3.72QDD17 pKa = 3.44NYY19 pKa = 10.24EE20 pKa = 4.12TGCYY24 pKa = 9.68GGSRR28 pKa = 11.84YY29 pKa = 10.15VFDD32 pKa = 6.03DD33 pKa = 3.39NFTVEE38 pKa = 4.68FNSTQDD44 pKa = 3.68LLEE47 pKa = 4.22KK48 pKa = 10.49LGEE51 pKa = 4.17YY52 pKa = 10.22LSSAFDD58 pKa = 3.4VDD60 pKa = 4.53SEE62 pKa = 4.76DD63 pKa = 4.29FVKK66 pKa = 10.58HH67 pKa = 4.86AHH69 pKa = 6.57NEE71 pKa = 3.87IEE73 pKa = 4.22NNRR76 pKa = 11.84FDD78 pKa = 4.09YY79 pKa = 10.96DD80 pKa = 3.35QSEE83 pKa = 4.45DD84 pKa = 3.98GEE86 pKa = 4.49GDD88 pKa = 3.85KK89 pKa = 10.67IDD91 pKa = 4.24ITPADD96 pKa = 3.87PDD98 pKa = 4.17GYY100 pKa = 9.61LARR103 pKa = 11.84YY104 pKa = 6.22TFYY107 pKa = 10.56IEE109 pKa = 4.64DD110 pKa = 3.46VRR112 pKa = 11.84EE113 pKa = 4.19VKK115 pKa = 9.21TVNYY119 pKa = 8.24TFSLL123 pKa = 3.87

Molecular weight: 14.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Y0AS84|A0A0Y0AS84_9CAUD Ribonucleoside-diphosphate reductase OS=Bacillus phage Eldridge OX=1776293 GN=Eldridge_0108 PE=3 SV=1
MM1 pKa = 7.69ARR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 8.34SQRR8 pKa = 11.84PKK10 pKa = 10.84LFQNKK15 pKa = 7.27QAPKK19 pKa = 9.97RR20 pKa = 11.84AMDD23 pKa = 3.73NVGKK27 pKa = 10.68AFVQKK32 pKa = 9.53TLDD35 pKa = 3.26AGMQAAQSQKK45 pKa = 9.14PQKK48 pKa = 10.17DD49 pKa = 3.47VQIKK53 pKa = 6.66VTRR56 pKa = 11.84KK57 pKa = 8.53PKK59 pKa = 9.61YY60 pKa = 10.45LEE62 pKa = 4.0VTEE65 pKa = 4.38KK66 pKa = 10.6RR67 pKa = 11.84LNKK70 pKa = 9.79MGVVDD75 pKa = 5.35LKK77 pKa = 11.1PFFAHH82 pKa = 6.39SPSRR86 pKa = 11.84KK87 pKa = 7.93MKK89 pKa = 10.59EE90 pKa = 3.59GGGWYY95 pKa = 9.34IRR97 pKa = 11.84IPIKK101 pKa = 10.61VKK103 pKa = 10.41KK104 pKa = 9.85KK105 pKa = 9.65DD106 pKa = 3.3MSRR109 pKa = 11.84RR110 pKa = 11.84MYY112 pKa = 10.76DD113 pKa = 3.15QLRR116 pKa = 11.84MINISPDD123 pKa = 3.08NQRR126 pKa = 11.84TVISDD131 pKa = 3.31YY132 pKa = 11.17LYY134 pKa = 11.02DD135 pKa = 3.87RR136 pKa = 11.84RR137 pKa = 11.84QASDD141 pKa = 3.53ASLLNYY147 pKa = 9.71TPVSYY152 pKa = 10.98NITKK156 pKa = 10.44QKK158 pKa = 8.18TGKK161 pKa = 9.98RR162 pKa = 11.84KK163 pKa = 7.96HH164 pKa = 5.91TYY166 pKa = 7.64VAYY169 pKa = 8.84RR170 pKa = 11.84TVSDD174 pKa = 4.28KK175 pKa = 11.52SPTSSWIVNRR185 pKa = 11.84DD186 pKa = 3.16KK187 pKa = 11.74VNTDD191 pKa = 3.24DD192 pKa = 3.77TSKK195 pKa = 9.93TFIRR199 pKa = 11.84NVNRR203 pKa = 11.84LMKK206 pKa = 10.07WKK208 pKa = 10.52AKK210 pKa = 10.47NGWEE214 pKa = 3.96

Molecular weight: 25.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

231

0

231

49026

22

1464

212.2

23.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.621 ± 0.151

0.824 ± 0.072

6.325 ± 0.116

7.3 ± 0.28

4.082 ± 0.109

6.788 ± 0.279

1.807 ± 0.096

6.458 ± 0.151

7.319 ± 0.225

8.081 ± 0.181

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.656 ± 0.1

5.566 ± 0.183

3.239 ± 0.146

3.69 ± 0.132

4.239 ± 0.125

6.117 ± 0.187

6.562 ± 0.246

6.919 ± 0.157

1.008 ± 0.052

4.4 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski