Rhodobacteraceae bacterium PARR1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3887 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257EZZ9|A0A257EZZ9_9RHOB Urea carboxylase OS=Rhodobacteraceae bacterium PARR1 OX=2015578 GN=CFE34_04985 PE=4 SV=1
MM1 pKa = 7.32LRR3 pKa = 11.84CAVPVILLACAPLPALANDD22 pKa = 3.85ADD24 pKa = 4.06AMRR27 pKa = 11.84QAISFCFDD35 pKa = 3.17TEE37 pKa = 4.33GDD39 pKa = 3.82VEE41 pKa = 4.41ALTEE45 pKa = 4.15RR46 pKa = 11.84VKK48 pKa = 11.19SDD50 pKa = 2.06GWTAEE55 pKa = 3.97RR56 pKa = 11.84DD57 pKa = 3.57EE58 pKa = 4.67EE59 pKa = 4.13MGLVNFYY66 pKa = 10.49TEE68 pKa = 4.27ASADD72 pKa = 3.6TFGFFATDD80 pKa = 3.28GSFCHH85 pKa = 6.53AEE87 pKa = 4.0SMTLSSEE94 pKa = 4.01DD95 pKa = 3.09TAMILQEE102 pKa = 4.06VVAEE106 pKa = 3.95YY107 pKa = 10.37EE108 pKa = 3.96YY109 pKa = 11.48DD110 pKa = 3.63FDD112 pKa = 6.25KK113 pKa = 11.56DD114 pKa = 4.87DD115 pKa = 4.27MGCTMLSFAGFGTATITSGGQDD137 pKa = 3.48PVCGAEE143 pKa = 4.02NNSAVRR149 pKa = 11.84FNFEE153 pKa = 3.48

Molecular weight: 16.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257ES54|A0A257ES54_9RHOB Amidohydro-rel domain-containing protein OS=Rhodobacteraceae bacterium PARR1 OX=2015578 GN=CFE34_19360 PE=4 SV=1
TT1 pKa = 6.87VVTATNRR8 pKa = 11.84AGTTSVAMTNRR19 pKa = 11.84AAMTNRR25 pKa = 11.84VAKK28 pKa = 8.88TSRR31 pKa = 11.84VAKK34 pKa = 8.48TSRR37 pKa = 11.84VAKK40 pKa = 9.62TVGTTSPAAKK50 pKa = 9.64IAVPKK55 pKa = 10.33IAVLTRR61 pKa = 11.84GMNARR66 pKa = 11.84PQLLRR71 pKa = 11.84RR72 pKa = 11.84SPP74 pKa = 3.34

Molecular weight: 7.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3887

0

3887

1192774

28

4507

306.9

33.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.446 ± 0.058

0.827 ± 0.012

6.045 ± 0.038

5.015 ± 0.034

3.583 ± 0.026

8.988 ± 0.05

2.008 ± 0.022

4.83 ± 0.026

2.852 ± 0.031

10.334 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.737 ± 0.024

2.381 ± 0.024

5.349 ± 0.032

3.174 ± 0.022

6.836 ± 0.036

4.842 ± 0.025

5.712 ± 0.036

7.606 ± 0.031

1.426 ± 0.018

2.007 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski