Rhizobium phage RL2RES

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; unclassified Ackermannviridae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9J1M5|A0A6B9J1M5_9CAUD Putative tail sheath stabilizer and completion protein OS=Rhizobium phage RL2RES OX=103371 GN=RL2RES_236 PE=4 SV=1
MM1 pKa = 7.38TWQQGMVNFLTIIFGIIAGALFNNLGEE28 pKa = 4.7APDD31 pKa = 3.47LTMGQRR37 pKa = 11.84YY38 pKa = 8.55FYY40 pKa = 10.2FAGFIVSMIIVLYY53 pKa = 10.5SLYY56 pKa = 10.37KK57 pKa = 10.58ASNEE61 pKa = 4.01MFGDD65 pKa = 4.69DD66 pKa = 5.89DD67 pKa = 5.55DD68 pKa = 4.54LTPAA72 pKa = 4.5

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9J1N3|A0A6B9J1N3_9CAUD Uncharacterized protein OS=Rhizobium phage RL2RES OX=103371 GN=RL2RES_207 PE=4 SV=1
MM1 pKa = 6.54STYY4 pKa = 10.53RR5 pKa = 11.84FCGCTKK11 pKa = 9.46CKK13 pKa = 9.99KK14 pKa = 8.6MRR16 pKa = 11.84RR17 pKa = 11.84KK18 pKa = 10.32GLVLGTRR25 pKa = 11.84NMGFWLTRR33 pKa = 11.84FGRR36 pKa = 11.84LEE38 pKa = 4.12YY39 pKa = 10.93YY40 pKa = 10.43LGPVSDD46 pKa = 4.34YY47 pKa = 10.76VRR49 pKa = 11.84HH50 pKa = 5.81PRR52 pKa = 11.84FFGRR56 pKa = 11.84RR57 pKa = 11.84PDD59 pKa = 3.35PKK61 pKa = 10.38FNKK64 pKa = 9.97KK65 pKa = 9.71EE66 pKa = 3.84IQEE69 pKa = 4.09

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

261

0

261

49062

37

1708

188.0

21.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.439 ± 0.163

0.85 ± 0.058

6.948 ± 0.13

6.973 ± 0.236

5.073 ± 0.136

6.196 ± 0.222

1.849 ± 0.095

6.669 ± 0.123

6.901 ± 0.206

7.678 ± 0.146

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.11

5.246 ± 0.109

3.441 ± 0.094

3.147 ± 0.097

5.132 ± 0.139

6.612 ± 0.183

6.155 ± 0.235

7.028 ± 0.173

1.276 ± 0.065

3.879 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski