Rhodobacter sp. CACIA14H1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobacter; unclassified Rhodobacter

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3570 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V7EJG3|V7EJG3_9RHOB Uncharacterized protein OS=Rhodobacter sp. CACIA14H1 OX=1408890 GN=Q27BPR15_13750 PE=4 SV=1
MM1 pKa = 7.77LFLLLAAFGLPANALNLSGASVEE24 pKa = 5.16LIYY27 pKa = 10.86SDD29 pKa = 3.87FGSYY33 pKa = 10.32GSAPVGSGVEE43 pKa = 3.87FDD45 pKa = 4.15EE46 pKa = 5.49GFGALTYY53 pKa = 10.88DD54 pKa = 3.71VDD56 pKa = 4.0PANNTVTLHH65 pKa = 6.74LSNSNPWGDD74 pKa = 3.45TTATLRR80 pKa = 11.84FSGGSVTDD88 pKa = 3.96FTSVAVTSNTTGQTYY103 pKa = 7.37TASVSGKK110 pKa = 10.02DD111 pKa = 3.56LVITLPVNSGAGDD124 pKa = 3.58VMFTLSDD131 pKa = 3.66TPLDD135 pKa = 3.91TVQPTVTGLTASSGRR150 pKa = 11.84YY151 pKa = 9.59GIGQTVQVSVSFSEE165 pKa = 4.38VVYY168 pKa = 9.36VTGTPQLTLEE178 pKa = 4.32TGATDD183 pKa = 3.89RR184 pKa = 11.84VASYY188 pKa = 11.11LSGSGTSTLVFSYY201 pKa = 9.36TVQAGDD207 pKa = 3.25TAADD211 pKa = 3.8LDD213 pKa = 4.25YY214 pKa = 11.61VAINSLALNGGTIRR228 pKa = 11.84DD229 pKa = 3.65AASNNAVLVLPSPGAANSLGANANIIVDD257 pKa = 3.96GVVPTVSSVTSPTSNGTYY275 pKa = 8.7KK276 pKa = 10.53TGDD279 pKa = 3.7TISVVVNFSEE289 pKa = 4.57TVIVTGTPQLTLEE302 pKa = 4.44TGLTDD307 pKa = 4.15RR308 pKa = 11.84SASYY312 pKa = 11.11SSGSGTSALTFTYY325 pKa = 9.54TVQSGDD331 pKa = 3.11TTADD335 pKa = 3.34LDD337 pKa = 4.26YY338 pKa = 11.45VATNSLALNGGSIRR352 pKa = 11.84DD353 pKa = 3.57EE354 pKa = 4.51AGNNATLTLASPGAANSLGANRR376 pKa = 11.84NIVIDD381 pKa = 5.08AISPTVTSVTSTTANDD397 pKa = 3.74TYY399 pKa = 11.18KK400 pKa = 10.41IGDD403 pKa = 3.91VISISVNFSEE413 pKa = 4.7TVTVTGTPQLTLEE426 pKa = 4.32TGATDD431 pKa = 3.52RR432 pKa = 11.84VVNYY436 pKa = 10.43ASGSGTGTLVFSYY449 pKa = 9.2TVQAGDD455 pKa = 3.22QSADD459 pKa = 3.24LDD461 pKa = 4.35YY462 pKa = 11.58ASTAALALNGGTIRR476 pKa = 11.84DD477 pKa = 3.74AAGNAATLTLASPGSANSLGANKK500 pKa = 10.27NIVIDD505 pKa = 4.11GVRR508 pKa = 11.84PAVTSIAPAGSTFGGASSVTFSVTFSEE535 pKa = 4.97TVTGVTPDD543 pKa = 3.99DD544 pKa = 3.79FALTVTGTAAGTIVSVSHH562 pKa = 6.69SGTTSDD568 pKa = 3.54VTVGSISGDD577 pKa = 3.02GTLRR581 pKa = 11.84LDD583 pKa = 3.99LRR585 pKa = 11.84GSTDD589 pKa = 2.41ICC591 pKa = 4.89

Molecular weight: 59.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V7EI79|V7EI79_9RHOB DNA polymerase III subunit epsilon (Fragment) OS=Rhodobacter sp. CACIA14H1 OX=1408890 GN=Q27BPR15_14575 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3570

0

3570

1060311

29

3616

297.0

32.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.528 ± 0.065

0.871 ± 0.016

5.805 ± 0.052

5.464 ± 0.037

3.596 ± 0.028

9.212 ± 0.071

2.042 ± 0.022

4.68 ± 0.031

2.77 ± 0.037

10.292 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.735 ± 0.023

2.25 ± 0.024

5.379 ± 0.034

2.886 ± 0.022

7.228 ± 0.042

4.602 ± 0.024

5.485 ± 0.038

7.666 ± 0.041

1.473 ± 0.023

2.035 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski