Megasphaera cerevisiae DSM 20462

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Megasphaera; Megasphaera cerevisiae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J6WVS0|A0A0J6WVS0_9FIRM L-xylulokinase OS=Megasphaera cerevisiae DSM 20462 OX=1122219 GN=AB840_11015 PE=4 SV=1
MM1 pKa = 7.42NPSTFQNLTGSDD13 pKa = 3.42GMFTFNFFCEE23 pKa = 4.26SLLGALHH30 pKa = 6.26TLAHH34 pKa = 5.61VMEE37 pKa = 5.75DD38 pKa = 3.47NQLDD42 pKa = 3.96MPAEE46 pKa = 4.39ASQIPDD52 pKa = 3.24MLAEE56 pKa = 4.46MGNSLSDD63 pKa = 4.03DD64 pKa = 3.92YY65 pKa = 11.85CDD67 pKa = 4.44GKK69 pKa = 10.74IDD71 pKa = 4.01LSRR74 pKa = 11.84FKK76 pKa = 11.28DD77 pKa = 3.57EE78 pKa = 5.72LLDD81 pKa = 3.54FHH83 pKa = 6.5KK84 pKa = 9.65TAFAIDD90 pKa = 3.85DD91 pKa = 3.63QMTSVIADD99 pKa = 3.52GDD101 pKa = 3.89DD102 pKa = 3.31TLQYY106 pKa = 10.89YY107 pKa = 8.58YY108 pKa = 10.3FVYY111 pKa = 9.37MQGISLFLPNMLDD124 pKa = 4.48AIGHH128 pKa = 6.37DD129 pKa = 4.0LPEE132 pKa = 5.61DD133 pKa = 3.65VDD135 pKa = 4.12PADD138 pKa = 4.02FMNEE142 pKa = 3.65ILSDD146 pKa = 4.19FAALTEE152 pKa = 4.37TQQQ155 pKa = 3.45

Molecular weight: 17.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J6X194|A0A0J6X194_9FIRM 6 7-dimethyl-8-ribityllumazine synthase OS=Megasphaera cerevisiae DSM 20462 OX=1122219 GN=ribH PE=3 SV=1
MM1 pKa = 7.81ICPKK5 pKa = 10.39CGTEE9 pKa = 3.96LRR11 pKa = 11.84DD12 pKa = 3.74GVRR15 pKa = 11.84MCPICGTQQVLPPQPPKK32 pKa = 9.77YY33 pKa = 8.53TRR35 pKa = 11.84SNQRR39 pKa = 11.84IFSKK43 pKa = 9.71TRR45 pKa = 11.84IISVIIVALFLIIGLWRR62 pKa = 11.84VIMRR66 pKa = 4.64

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2843

0

2843

863096

31

2682

303.6

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.812 ± 0.056

1.463 ± 0.018

5.503 ± 0.035

5.927 ± 0.046

3.943 ± 0.033

7.511 ± 0.048

2.295 ± 0.023

7.47 ± 0.047

5.764 ± 0.045

8.924 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.118 ± 0.024

3.869 ± 0.043

3.795 ± 0.027

3.767 ± 0.032

4.771 ± 0.043

5.561 ± 0.031

5.706 ± 0.044

7.081 ± 0.04

1.002 ± 0.019

3.718 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski