Ruminococcaceae bacterium KH2T8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2593 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0PRT3|A0A1I0PRT3_9FIRM Uncharacterized protein OS=Ruminococcaceae bacterium KH2T8 OX=1855400 GN=SAMN05216413_1370 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.3KK3 pKa = 9.77IVSTVLCLSMVLAFASCSDD22 pKa = 3.43ASDD25 pKa = 3.55KK26 pKa = 10.91KK27 pKa = 10.66RR28 pKa = 11.84SSRR31 pKa = 11.84DD32 pKa = 2.82GDD34 pKa = 3.52ILTEE38 pKa = 4.3AEE40 pKa = 4.04DD41 pKa = 3.66EE42 pKa = 4.21KK43 pKa = 11.14RR44 pKa = 11.84DD45 pKa = 3.74RR46 pKa = 11.84DD47 pKa = 3.58EE48 pKa = 6.13SEE50 pKa = 4.1DD51 pKa = 4.1DD52 pKa = 3.66EE53 pKa = 5.43VEE55 pKa = 4.16EE56 pKa = 4.43TTATSQEE63 pKa = 4.08TTAATATSSAAVGGRR78 pKa = 11.84VTFSTVDD85 pKa = 3.25RR86 pKa = 11.84DD87 pKa = 3.69GNTFDD92 pKa = 5.45DD93 pKa = 4.97SVFADD98 pKa = 3.66HH99 pKa = 7.1QLTLINFWEE108 pKa = 5.01PWCGPCVEE116 pKa = 5.58EE117 pKa = 6.0IPDD120 pKa = 3.6LQTIYY125 pKa = 10.99EE126 pKa = 4.24NYY128 pKa = 9.91SDD130 pKa = 4.63QGLLVIGVYY139 pKa = 9.83SEE141 pKa = 4.94EE142 pKa = 4.1YY143 pKa = 9.84MEE145 pKa = 4.74EE146 pKa = 4.11DD147 pKa = 2.91VDD149 pKa = 5.21AVLSDD154 pKa = 3.65GGVTYY159 pKa = 9.79PILRR163 pKa = 11.84YY164 pKa = 8.28TSEE167 pKa = 3.7FDD169 pKa = 3.33QYY171 pKa = 11.4QSGYY175 pKa = 10.08VPTTILVDD183 pKa = 3.85SNGNIIDD190 pKa = 3.63TGEE193 pKa = 4.19SYY195 pKa = 11.0EE196 pKa = 4.51GLDD199 pKa = 3.13STLIVGSRR207 pKa = 11.84SYY209 pKa = 10.61TEE211 pKa = 3.68WEE213 pKa = 4.38AIVNQYY219 pKa = 10.88LGAA222 pKa = 5.2

Molecular weight: 24.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0QI20|A0A1I0QI20_9FIRM Uncharacterized protein OS=Ruminococcaceae bacterium KH2T8 OX=1855400 GN=SAMN05216413_1753 PE=4 SV=1
MM1 pKa = 7.75AEE3 pKa = 3.62NRR5 pKa = 11.84APKK8 pKa = 10.08AGRR11 pKa = 11.84EE12 pKa = 3.86FSGNMRR18 pKa = 11.84QRR20 pKa = 11.84RR21 pKa = 11.84SRR23 pKa = 11.84RR24 pKa = 11.84KK25 pKa = 8.89SCTFCAEE32 pKa = 3.89KK33 pKa = 11.16VEE35 pKa = 5.09VIDD38 pKa = 6.04FMDD41 pKa = 3.87ATRR44 pKa = 11.84LRR46 pKa = 11.84KK47 pKa = 9.96YY48 pKa = 9.61ISEE51 pKa = 4.13RR52 pKa = 11.84GKK54 pKa = 10.12ILPKK58 pKa = 10.67RR59 pKa = 11.84MTGTCAKK66 pKa = 9.56HH67 pKa = 5.37QRR69 pKa = 11.84EE70 pKa = 4.47LTTAIKK76 pKa = 10.32RR77 pKa = 11.84ARR79 pKa = 11.84QIALLPYY86 pKa = 9.77IAEE89 pKa = 4.14

Molecular weight: 10.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2593

0

2593

889011

29

3243

342.9

38.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.556 ± 0.051

1.535 ± 0.019

6.939 ± 0.046

7.073 ± 0.047

4.371 ± 0.036

7.008 ± 0.042

1.6 ± 0.019

7.703 ± 0.044

5.915 ± 0.064

8.395 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.917 ± 0.023

4.233 ± 0.037

3.503 ± 0.027

2.293 ± 0.021

4.327 ± 0.036

6.587 ± 0.044

5.812 ± 0.066

6.985 ± 0.034

0.882 ± 0.016

4.365 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski