Lake Sarah-associated circular virus-3

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQJ5|A0A140AQJ5_9VIRU Uncharacterized protein OS=Lake Sarah-associated circular virus-3 OX=1685757 PE=4 SV=1
MM1 pKa = 7.47NLLLCLCNVTQFNSMSSDD19 pKa = 3.29TVKK22 pKa = 10.92CGVATLDD29 pKa = 3.7PFRR32 pKa = 11.84PAAKK36 pKa = 9.79GACLPTGSCPATYY49 pKa = 9.81RR50 pKa = 11.84ASAVIRR56 pKa = 11.84VDD58 pKa = 4.0GVLGPQGQGIIMVNPSVASDD78 pKa = 4.41AACLWYY84 pKa = 11.1SNGSTFTQSPVVIGNSAYY102 pKa = 11.11VNGTGLTGPVNGLPTLITPGLTQATATSLPFQSDD136 pKa = 2.84AMFGFNASTALPLPASGPPQVRR158 pKa = 11.84ARR160 pKa = 11.84IVSCGVKK167 pKa = 8.76ITFSGTTLNDD177 pKa = 3.2GGVAYY182 pKa = 10.48CLVDD186 pKa = 3.78PQHH189 pKa = 6.65EE190 pKa = 4.21NLIEE194 pKa = 4.11LGINNYY200 pKa = 10.19LSLFTSCKK208 pKa = 8.77IQRR211 pKa = 11.84LSLRR215 pKa = 11.84DD216 pKa = 3.82TIALNLAPVTRR227 pKa = 11.84NQQDD231 pKa = 3.2LSSAYY236 pKa = 10.49DD237 pKa = 3.73EE238 pKa = 4.9VPLGSIFGGSLNGGYY253 pKa = 9.64IYY255 pKa = 10.92SPFSMPNPTSNFGYY269 pKa = 10.18GYY271 pKa = 9.43PNVGQSTQSCVPCDD285 pKa = 3.51AFNCAKK291 pKa = 10.42AASTLLYY298 pKa = 9.72PLSRR302 pKa = 11.84RR303 pKa = 11.84NQRR306 pKa = 11.84VIPWTVNNSTTTGLWSGSVAGGGLITWTSIAPDD339 pKa = 3.47VGASGMIMSGTVSGLGTIDD358 pKa = 3.38VEE360 pKa = 4.4GVIWYY365 pKa = 9.53RR366 pKa = 11.84YY367 pKa = 10.01DD368 pKa = 3.29NNSWYY373 pKa = 10.43FSLTSGVPTITPFNFTNWAITGYY396 pKa = 8.6WCEE399 pKa = 3.86PSVIGAVIIQAGTAMAGQTFHH420 pKa = 7.84IEE422 pKa = 4.15YY423 pKa = 10.05VVHH426 pKa = 6.73CEE428 pKa = 3.77YY429 pKa = 11.14SGVGVQGRR437 pKa = 11.84TEE439 pKa = 4.27NIIPDD444 pKa = 3.97PEE446 pKa = 4.89GLAGVHH452 pKa = 6.51AVLDD456 pKa = 4.04LCRR459 pKa = 11.84EE460 pKa = 4.06SAGQHH465 pKa = 4.2EE466 pKa = 4.68RR467 pKa = 11.84ANIKK471 pKa = 10.69DD472 pKa = 3.87FVSAASSKK480 pKa = 10.16LVKK483 pKa = 10.49SGALAVKK490 pKa = 9.75LGEE493 pKa = 4.24VASVLGKK500 pKa = 10.46RR501 pKa = 11.84FNN503 pKa = 3.58

Molecular weight: 52.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G9I2|A0A126G9I2_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-3 OX=1685757 PE=3 SV=1
MM1 pKa = 7.67AKK3 pKa = 10.08YY4 pKa = 9.26FKK6 pKa = 9.87PSKK9 pKa = 10.2KK10 pKa = 9.93FPTRR14 pKa = 11.84EE15 pKa = 3.3HH16 pKa = 5.56YY17 pKa = 10.39RR18 pKa = 11.84NYY20 pKa = 10.11RR21 pKa = 11.84EE22 pKa = 3.92AMGIRR27 pKa = 11.84GKK29 pKa = 10.58KK30 pKa = 8.23SSVYY34 pKa = 10.21KK35 pKa = 10.46RR36 pKa = 11.84LRR38 pKa = 11.84GNKK41 pKa = 7.58LTRR44 pKa = 11.84STRR47 pKa = 11.84YY48 pKa = 8.59KK49 pKa = 9.81QKK51 pKa = 10.47KK52 pKa = 9.25FGFKK56 pKa = 9.74RR57 pKa = 11.84SSYY60 pKa = 11.37AKK62 pKa = 10.15GLKK65 pKa = 10.25LPLSTTCPKK74 pKa = 11.1NMLFLSNAFIEE85 pKa = 4.35YY86 pKa = 10.06FKK88 pKa = 10.93ISLKK92 pKa = 9.9MLNDD96 pKa = 3.57EE97 pKa = 4.69PFKK100 pKa = 11.62DD101 pKa = 3.57MLALVSRR108 pKa = 11.84IPTYY112 pKa = 10.36RR113 pKa = 11.84ALDD116 pKa = 3.62AFATLITCTPFSKK129 pKa = 9.74YY130 pKa = 7.97TAVGDD135 pKa = 4.25AVQEE139 pKa = 4.04MCRR142 pKa = 11.84QFLVLFKK149 pKa = 10.22TAIAHH154 pKa = 6.53AEE156 pKa = 4.12SEE158 pKa = 4.3LLVPGAHH165 pKa = 4.55QKK167 pKa = 8.88MRR169 pKa = 11.84EE170 pKa = 4.01VEE172 pKa = 3.95HH173 pKa = 6.98ALRR176 pKa = 11.84EE177 pKa = 4.31HH178 pKa = 5.69TKK180 pKa = 9.74EE181 pKa = 4.09ANVEE185 pKa = 3.95LAEE188 pKa = 5.1HH189 pKa = 6.71ILHH192 pKa = 6.95EE193 pKa = 4.67RR194 pKa = 11.84MQQANEE200 pKa = 3.76QMEE203 pKa = 4.55KK204 pKa = 10.45FGAHH208 pKa = 6.25MGDD211 pKa = 3.1KK212 pKa = 10.27VGKK215 pKa = 9.31LARR218 pKa = 11.84ARR220 pKa = 11.84NEE222 pKa = 3.53VMEE225 pKa = 4.21RR226 pKa = 11.84AKK228 pKa = 11.09VEE230 pKa = 4.28MVDD233 pKa = 3.84GTQSAAAQSGDD244 pKa = 3.59MYY246 pKa = 10.73HH247 pKa = 7.0KK248 pKa = 9.89SHH250 pKa = 6.55HH251 pKa = 6.69HH252 pKa = 5.29GHH254 pKa = 6.49HH255 pKa = 6.37KK256 pKa = 10.53GINLKK261 pKa = 10.37SVSRR265 pKa = 11.84ALNHH269 pKa = 6.51AEE271 pKa = 3.85KK272 pKa = 10.6SVMSGAEE279 pKa = 3.85SVMRR283 pKa = 11.84TVTQTVASDD292 pKa = 3.9PEE294 pKa = 4.42SVAMFMM300 pKa = 5.4

Molecular weight: 34.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1202

300

503

400.7

44.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.987 ± 0.898

2.163 ± 0.445

4.576 ± 1.21

4.576 ± 1.169

5.075 ± 1.022

7.404 ± 1.801

3.661 ± 1.492

5.408 ± 0.841

4.576 ± 1.627

7.82 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.919

4.077 ± 1.079

5.74 ± 0.779

3.078 ± 0.367

4.992 ± 0.962

7.737 ± 1.199

7.571 ± 0.63

7.072 ± 0.683

1.082 ± 0.323

3.078 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski