Tomato bright yellow mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 9.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9SAJ1|R9SAJ1_9GEMI Replication enhancer OS=Tomato bright yellow mosaic virus OX=1340746 PE=3 SV=1
MM1 pKa = 8.18DD2 pKa = 4.39SRR4 pKa = 11.84TGEE7 pKa = 4.74HH8 pKa = 5.44ITAPQAQNGVHH19 pKa = 6.02IWEE22 pKa = 4.94IPNPLYY28 pKa = 10.65FKK30 pKa = 10.51IIDD33 pKa = 3.98VEE35 pKa = 4.34DD36 pKa = 3.97PPYY39 pKa = 7.59TTTRR43 pKa = 11.84IYY45 pKa = 10.71HH46 pKa = 4.85LQIRR50 pKa = 11.84FNHH53 pKa = 5.21NTRR56 pKa = 11.84KK57 pKa = 10.19ALGLHH62 pKa = 5.74KK63 pKa = 10.43AYY65 pKa = 10.94LNFHH69 pKa = 6.61IWTTSITASGTRR81 pKa = 11.84YY82 pKa = 9.96LNRR85 pKa = 11.84FRR87 pKa = 11.84LLVLAYY93 pKa = 9.88LDD95 pKa = 4.17NIGVISINNVIRR107 pKa = 11.84AVRR110 pKa = 11.84FATDD114 pKa = 2.26KK115 pKa = 11.1HH116 pKa = 6.53YY117 pKa = 11.01IHH119 pKa = 6.69NVLEE123 pKa = 3.83NHH125 pKa = 6.36EE126 pKa = 4.67IKK128 pKa = 10.75FKK130 pKa = 11.08LYY132 pKa = 10.76

Molecular weight: 15.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9SA01|R9SA01_9GEMI Capsid protein OS=Tomato bright yellow mosaic virus OX=1340746 PE=3 SV=1
MM1 pKa = 7.92PKK3 pKa = 9.84TISSLILSALYY14 pKa = 9.85RR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 10.2KK18 pKa = 10.01HH19 pKa = 5.55FPNYY23 pKa = 10.39LPLQYY28 pKa = 10.97QSINYY33 pKa = 9.69SFVLPGNYY41 pKa = 8.81TKK43 pKa = 10.16MGNLICMSSSNSKK56 pKa = 10.7GNSNVPTRR64 pKa = 11.84DD65 pKa = 3.17CSTLHH70 pKa = 6.0LQPGQHH76 pKa = 6.21ISILTYY82 pKa = 9.51RR83 pKa = 11.84QLRR86 pKa = 11.84AAQMLKK92 pKa = 8.69HH93 pKa = 5.38TWKK96 pKa = 8.82KK97 pKa = 9.17TEE99 pKa = 3.42ISLIMEE105 pKa = 4.9CSKK108 pKa = 11.67SMADD112 pKa = 3.3QLEE115 pKa = 4.63EE116 pKa = 4.21VSSLPTTRR124 pKa = 11.84MPRR127 pKa = 11.84SSTQDD132 pKa = 3.21QSWRR136 pKa = 11.84PSIYY140 pKa = 10.35LGRR143 pKa = 11.84NNPKK147 pKa = 9.53ISSFNIITSAQILNGSSLRR166 pKa = 11.84LQNHH170 pKa = 5.7GFLRR174 pKa = 11.84FNSPHH179 pKa = 6.63SLTFHH184 pKa = 6.63RR185 pKa = 11.84RR186 pKa = 11.84CNSGQMNISEE196 pKa = 4.42EE197 pKa = 4.14VPLRR201 pKa = 11.84GRR203 pKa = 11.84RR204 pKa = 11.84DD205 pKa = 3.45LSVLSLRR212 pKa = 11.84VIPEE216 pKa = 3.6RR217 pKa = 11.84EE218 pKa = 3.89RR219 pKa = 11.84RR220 pKa = 11.84CGHH223 pKa = 5.5VLWGSTIILVVISTLIQNAIQMRR246 pKa = 11.84PSTTLLTTWTPNILKK261 pKa = 10.61LNTGKK266 pKa = 10.43NSLGPKK272 pKa = 8.33KK273 pKa = 9.22TGNPIANMVVQFKK286 pKa = 10.53LKK288 pKa = 10.1EE289 pKa = 4.04GSPQSCSAIQEE300 pKa = 3.81RR301 pKa = 11.84DD302 pKa = 2.97PAINVSSTKK311 pKa = 10.51RR312 pKa = 11.84KK313 pKa = 7.07TQLSKK318 pKa = 10.51IGRR321 pKa = 11.84STT323 pKa = 3.06

Molecular weight: 36.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

858

132

323

214.5

24.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.196 ± 0.624

1.282 ± 0.445

2.797 ± 0.543

3.497 ± 0.196

3.263 ± 0.461

5.594 ± 0.547

3.846 ± 0.778

7.459 ± 0.968

5.944 ± 0.348

8.392 ± 1.604

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.479

6.177 ± 1.13

5.128 ± 0.432

4.312 ± 0.766

8.392 ± 0.609

9.207 ± 1.979

7.11 ± 0.662

4.895 ± 1.272

1.632 ± 0.174

4.079 ± 0.89
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski