European catfish virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus; Epizootic haematopoietic necrosis virus

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 135 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2BFW2|I2BFW2_9VIRU Uncharacterized protein OS=European catfish virus OX=84739 GN=129L PE=4 SV=1
MM1 pKa = 7.55TGPPEE6 pKa = 4.37GVLHH10 pKa = 5.18VLRR13 pKa = 11.84RR14 pKa = 11.84FAYY17 pKa = 10.0RR18 pKa = 11.84FVKK21 pKa = 10.21EE22 pKa = 4.07EE23 pKa = 4.02KK24 pKa = 10.01FGVWMMIDD32 pKa = 3.69VEE34 pKa = 4.61DD35 pKa = 5.29KK36 pKa = 11.66SMTTLGSPSNDD47 pKa = 2.86FPGLVVSQLTADD59 pKa = 3.99DD60 pKa = 4.05GDD62 pKa = 4.72RR63 pKa = 11.84IHH65 pKa = 7.53GDD67 pKa = 3.29ALYY70 pKa = 10.99SDD72 pKa = 4.69YY73 pKa = 11.32VYY75 pKa = 10.69HH76 pKa = 7.91DD77 pKa = 3.36SMYY80 pKa = 10.72TDD82 pKa = 3.89LEE84 pKa = 4.25EE85 pKa = 4.62EE86 pKa = 3.88IVYY89 pKa = 7.25YY90 pKa = 10.78VCDD93 pKa = 4.76SIPEE97 pKa = 4.05HH98 pKa = 6.57SS99 pKa = 3.74

Molecular weight: 11.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165Y8A4|A0A165Y8A4_9VIRU Uncharacterized protein OS=European catfish virus OX=84739 PE=4 SV=1
MM1 pKa = 7.11YY2 pKa = 10.24SVRR5 pKa = 11.84NSGCSVGCSPRR16 pKa = 11.84QGASPIMFGPSLGAMLSAPVVRR38 pKa = 11.84ASSPVRR44 pKa = 11.84KK45 pKa = 9.68RR46 pKa = 11.84KK47 pKa = 10.2SLVKK51 pKa = 10.35RR52 pKa = 11.84KK53 pKa = 10.2SLVNRR58 pKa = 11.84KK59 pKa = 9.52SPVKK63 pKa = 10.36RR64 pKa = 11.84SPLKK68 pKa = 10.23KK69 pKa = 9.52RR70 pKa = 11.84SPVRR74 pKa = 11.84KK75 pKa = 9.74SPVKK79 pKa = 10.16RR80 pKa = 11.84KK81 pKa = 10.4SPVKK85 pKa = 10.23RR86 pKa = 11.84KK87 pKa = 10.33SPVKK91 pKa = 10.02KK92 pKa = 10.07RR93 pKa = 11.84SPVKK97 pKa = 10.14RR98 pKa = 11.84KK99 pKa = 10.37SPVKK103 pKa = 10.34RR104 pKa = 11.84SPLKK108 pKa = 10.26RR109 pKa = 11.84KK110 pKa = 10.23SPVKK114 pKa = 10.13RR115 pKa = 11.84KK116 pKa = 10.4SPVKK120 pKa = 10.23RR121 pKa = 11.84KK122 pKa = 10.33SPVKK126 pKa = 10.02KK127 pKa = 10.07RR128 pKa = 11.84SPVKK132 pKa = 10.14RR133 pKa = 11.84KK134 pKa = 10.37SPVKK138 pKa = 10.34RR139 pKa = 11.84SPLKK143 pKa = 10.58RR144 pKa = 11.84KK145 pKa = 10.06SPVNKK150 pKa = 9.77RR151 pKa = 11.84SPVKK155 pKa = 10.34RR156 pKa = 11.84KK157 pKa = 10.18SPLKK161 pKa = 10.38RR162 pKa = 11.84KK163 pKa = 10.03SPLKK167 pKa = 10.12KK168 pKa = 9.84RR169 pKa = 11.84SPVKK173 pKa = 10.16RR174 pKa = 11.84KK175 pKa = 10.37SPVKK179 pKa = 10.34RR180 pKa = 11.84SPLKK184 pKa = 10.58RR185 pKa = 11.84KK186 pKa = 10.06SPVNKK191 pKa = 9.77RR192 pKa = 11.84SPVKK196 pKa = 10.34RR197 pKa = 11.84KK198 pKa = 10.18SPLKK202 pKa = 10.38RR203 pKa = 11.84KK204 pKa = 10.03SPLKK208 pKa = 10.12KK209 pKa = 9.84RR210 pKa = 11.84SPVKK214 pKa = 10.16RR215 pKa = 11.84KK216 pKa = 10.34SPVKK220 pKa = 9.97RR221 pKa = 11.84KK222 pKa = 10.1SPAKK226 pKa = 9.61MRR228 pKa = 11.84QQVCFMTTKK237 pKa = 10.75

Molecular weight: 26.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

135

0

135

34463

40

1298

255.3

28.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.621 ± 0.289

2.04 ± 0.134

5.954 ± 0.184

5.571 ± 0.205

3.47 ± 0.187

7.347 ± 0.267

2.011 ± 0.111

3.317 ± 0.133

5.577 ± 0.266

8.215 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.98 ± 0.126

2.466 ± 0.119

6.163 ± 0.27

2.777 ± 0.213

7.643 ± 0.275

7.547 ± 0.265

5.565 ± 0.162

8.165 ± 0.242

1.419 ± 0.106

3.151 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski